PLK1 Monoclonal Antibody (35-206) | Invitrogen 37-7000 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PLK1 Monoclonal Antibody (35-206)

catalog :

37-7000

quantity :

100µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

35-206

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; loading ...; fig 1a	Yamanishi E, Hasegawa K, Fujita K, Ichinose S, Yagishita S, Murata M, et al. A novel form of necrosis, TRIAD, occurs in human Huntington's disease. Acta Neuropathol Commun. 2017;5:19 pubmed publisher
western blot; human; fig 1	Jayashree B, Srimany A, Jayaraman S, Bhutra A, Janakiraman N, Chitipothu S, et al. Monitoring of changes in lipid profiles during PLK1 knockdown in cancer cells using DESI MS. Anal Bioanal Chem. 2016;408:5623-32 pubmed publisher
western blot; rat; 1:500; fig 4	Mao Y, Tamura T, Yuki Y, Abe D, Tamada Y, Imoto S, et al. The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional repression through impaired RNA splicing. Cell Death Dis. 2016;7:e2207 pubmed publisher
flow cytometry; human; fig 2	Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
western blot; human; fig 5	Saini P, Li Y, Dobbelstein M. Wee1 is required to sustain ATR/Chk1 signaling upon replicative stress. Oncotarget. 2015;6:13072-87 pubmed
western blot; human; 1:100; fig 7	Fan Y, Zhao H, Liu J, Tan T, Ding T, Li R, et al. Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes. Sci Rep. 2015;5:8192 pubmed publisher
western blot; human western blot; mouse	Vassilopoulos A, Tominaga Y, Kim H, Lahusen T, Li B, Yu H, et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2015;34:3023-35 pubmed publisher
western blot; human; 1:2500	Akiyama M, Izumi H, Wang K, Yamaguchi T, Kuma A, Kitamura N, et al. Hypersensitivity to aurora kinase inhibitors in cells resistant against platinum- containing anticancer agents. Anticancer Agents Med Chem. 2014;14:1042-50 pubmed
western blot; human; fig s2	Nakamura T, Saito H, Takekawa M. SAPK pathways and p53 cooperatively regulate PLK4 activity and centrosome integrity under stress. Nat Commun. 2013;4:1775 pubmed publisher
western blot; human; 1:200; fig 5	Landsverk K, Patzke S, Rein I, Stokke C, Lyng H, De Angelis P, et al. Three independent mechanisms for arrest in G2 after ionizing radiation. Cell Cycle. 2011;10:819-29 pubmed
immunoprecipitation; human immunocytochemistry; human western blot; human	Fabbro M, Zhou B, Takahashi M, Sarcevic B, Lal P, Graham M, et al. Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis. Dev Cell. 2005;9:477-88 pubmed
	Clift D, Schuh M. A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes. Nat Commun. 2015;6:7217 pubmed publisher
	Zhang B, Huang B, Guan H, Zhang S, Xu Q, He X, et al. Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde. Toxicol Appl Pharmacol. 2011;252:307-17 pubmed publisher
	Wang Z, Udeshi N, Slawson C, Compton P, Sakabe K, Cheung W, et al. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal. 2010;3:ra2 pubmed publisher

product information

Product Type :

Antibody

Product Name :

PLK1 Monoclonal Antibody (35-206)

Catalog # :

37-7000

Quantity :

100µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay Dependent, Immunocytochemistry: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat

Clone :

35-206

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Immunogen :

Full-length human Plk1 (polo-like kinase 1)

Format :

Liquid

Applications w/Dilutions :

Aliases :

Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13, PLK1, PLK

more info or order :

Invitrogen product webpage