MSH2 Monoclonal Antibody (FE11) | Invitrogen 33-7900 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MSH2 Monoclonal Antibody (FE11)

catalog :

33-7900

quantity :

50µg

price :

US 275.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

FE11

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; tbl 2	Massalou D, Benizri E, Chevallier A, Duranton-Tanneur V, Pedeutour F, Benchimol D, et al. Peritoneal carcinomatosis of colorectal cancer: novel clinical and molecular outcomes. Am J Surg. 2016;: pubmed publisher
immunohistochemistry; human; 1:100; loading ...	Boulagnon C, Dudez O, Beaudoux O, Dalstein V, Kianmanesh R, Bouche O, et al. BRAFV600E Gene Mutation in Colonic Adenocarcinomas. Immunohistochemical Detection Using Tissue Microarray and Clinicopathologic Characteristics: An 86 Case Series. Appl Immunohistochem Mol Morphol. 2016;24:88-96 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; fig s1	Koussounadis A, Langdon S, Um I, Kay C, Francis K, Harrison D, et al. Dynamic modulation of phosphoprotein expression in ovarian cancer xenograft models. BMC Cancer. 2016;16:205 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200	Kim K, Wen X, Yang H, Kim W, Kang G. Prognostic Implication of M2 Macrophages Are Determined by the Proportional Balance of Tumor Associated Macrophages and Tumor Infiltrating Lymphocytes in Microsatellite-Unstable Gastric Carcinoma. PLoS ONE. 2015;10:e0144192 pubmed publisher
immunohistochemistry - paraffin section; human	Shin S, Yu E, Lee Y, Song Y, Seong M, Park S. Characteristics of hereditary nonpolyposis colorectal cancer patients with double primary cancers in endometrium and colorectum. Obstet Gynecol Sci. 2015;58:112-6 pubmed publisher
immunohistochemistry - paraffin section; human	Gatalica Z, Lilleberg S, Monzon F, Koul M, Bridge J, Knezetic J, et al. Renal medullary carcinomas: histopathologic phenotype associated with diverse genotypes. Hum Pathol. 2011;42:1979-88 pubmed publisher
immunohistochemistry - paraffin section; human; fig 3	Couronné L, Ruminy P, Waultier-Rascalou A, Rainville V, Cornic M, Picquenot J, et al. Mutation mismatch repair gene deletions in diffuse large B-cell lymphoma. Leuk Lymphoma. 2013;54:1079-86 pubmed publisher
immunohistochemistry - paraffin section; human; 210 mg/l	Yoon S, Ku J, Shin Y, Kim K, Choi J, Jang E, et al. Hereditary nonpolyposis colorectal cancer in endometrial cancer patients. Int J Cancer. 2008;122:1077-81 pubmed
immunohistochemistry - paraffin section; human; 1:250; fig 1	Li M, Liu L, Wang Z, Wang L, Liu Z, Xu G, et al. Overexpression of hMSH2 and hMLH1 protein in certain gastric cancers and their surrounding mucosae. Oncol Rep. 2008;19:401-6 pubmed
immunohistochemistry - paraffin section; human	Ricciardiello L, Ceccarelli C, Angiolini G, Pariali M, Chieco P, Paterini P, et al. High thymidylate synthase expression in colorectal cancer with microsatellite instability: implications for chemotherapeutic strategies. Clin Cancer Res. 2005;11:4234-40 pubmed
immunohistochemistry - paraffin section; human; 1:400; fig 4	Buttin B, Powell M, Mutch D, Rader J, Herzog T, Gibb R, et al. Increased risk for hereditary nonpolyposis colorectal cancer-associated synchronous and metachronous malignancies in patients with microsatellite instability-positive endometrial carcinoma lacking MLH1 promoter methylation. Clin Cancer Res. 2004;10:481-90 pubmed
immunohistochemistry - paraffin section; human	Chen Z, Ritter J, Wang H. Differential expression of alpha-methylacyl coenzyme A racemase in adenocarcinomas of the small and large intestines. Am J Surg Pathol. 2005;29:890-6 pubmed
immunohistochemistry - paraffin section; human; 1:200	Lee J, Cragun D, Thompson Z, Coppola D, Nicosia S, Akbari M, et al. Association between IHC and MSI testing to identify mismatch repair-deficient patients with ovarian cancer. Genet Test Mol Biomarkers. 2014;18:229-35 pubmed publisher
immunohistochemistry; human; 1:250; tbl 1	Li M, Zhang Q, Liu L, Lu W, Wei H, Li R, et al. Expression of the mismatch repair gene hMLH1 is enhanced in non-small cell lung cancer with EGFR mutations. PLoS ONE. 2013;8:e78500 pubmed publisher
immunohistochemistry - paraffin section; human; 1:400; fig 4	Martin S, McCabe N, Mullarkey M, Cummins R, Burgess D, Nakabeppu Y, et al. DNA polymerases as potential therapeutic targets for cancers deficient in the DNA mismatch repair proteins MSH2 or MLH1. Cancer Cell. 2010;17:235-48 pubmed publisher
immunohistochemistry - paraffin section; human; 1:400	Boman B, Walters R, Fields J, Kovatich A, Zhang T, Isenberg G, et al. Colonic crypt changes during adenoma development in familial adenomatous polyposis: immunohistochemical evidence for expansion of the crypt base cell population. Am J Pathol. 2004;165:1489-98 pubmed
immunohistochemistry - paraffin section; human; 1:140	Mylona E, Zarogiannos A, Nomikos A, Giannopoulou I, Nikolaou I, Zervas A, et al. Prognostic value of microsatellite instability determined by immunohistochemical staining of hMSH2 and hMSH6 in urothelial carcinoma of the bladder. APMIS. 2008;116:59-65 pubmed publisher
western blot; human; fig 7	Campos-Vega R, García-Gasca T, Guevara-González R, Ramos-Gomez M, Oomah B, Loarca-Pina G. Human gut flora-fermented nondigestible fraction from cooked bean ( Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells. J Agric Food Chem. 2012;60:12443-50 pubmed publisher
immunohistochemistry - paraffin section; human; fig 3	Helal T, Fadel M, El-Thobbani A, El-Sarhi A. Immunoexpression of p53 and hMSH2 in oral squamous cell carcinoma and oral dysplastic lesions in Yemen: relationship to oral risk habits and prognostic factors. Oral Oncol. 2012;48:120-4 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200	Permuth-Wey J, Boulware D, Valkov N, Livingston S, Nicosia S, Lee J, et al. Sampling strategies for tissue microarrays to evaluate biomarkers in ovarian cancer. Cancer Epidemiol Biomarkers Prev. 2009;18:28-34 pubmed publisher
immunohistochemistry - paraffin section; human; fig 1	Bendardaf R, Lamlum H, Ristamaki R, Syrjanen K, Pyrhonen S. Oncoprotein Bcl-2 and microsatellite instability are associated with disease-free survival and treatment response in colorectal cancer. Oncol Rep. 2008;20:999-1004 pubmed
immunohistochemistry; human; 1:100	Kerber R, Neklason D, Samowitz W, Burt R. Frequency of familial colon cancer and hereditary nonpolyposis colorectal cancer (Lynch syndrome) in a large population database. Fam Cancer. 2005;4:239-44 pubmed
immunohistochemistry - paraffin section; human	Cossio S, Koehler-Santos P, Pessini S, Mónego H, Edelweiss M, Meurer L, et al. Clinical and histomolecular endometrial tumor characterization of patients at-risk for Lynch syndrome in South of Brazil. Fam Cancer. 2010;9:131-9 pubmed publisher
immunohistochemistry - paraffin section; human; 1:150	Arabi H, Guan H, Kumar S, Cote M, Bandyopadhyay S, Bryant C, et al. Impact of microsatellite instability (MSI) on survival in high grade endometrial carcinoma. Gynecol Oncol. 2009;113:153-8 pubmed publisher
immunohistochemistry; human; 1:100; fig 1	Dadone B, Ambrosetti D, Carpentier X, Duranton-Tanneur V, Burel-Vandenbos F, Amiel J, et al. A renal metanephric adenoma showing both a 2p16e24 deletion and BRAF V600E mutation: a synergistic role for a tumor suppressor gene on chromosome 2p and BRAF activation?. Cancer Genet. 2013;206:347-52 pubmed
immunohistochemistry - paraffin section; human	Edelmann J, Richter K, Hanel C, Hering S, Horn L. X chromosomal and autosomal loss of heterozygosity and microsatellite instability in human cervical carcinoma. Int J Gynecol Cancer. 2006;16:1248-53 pubmed
immunohistochemistry - paraffin section; human; fig 2	Boulagnon C, Jazeron J, Diaz-Cives A, Ehrhard F, Bouche O, Diebold M. Filiform polyposis: A benign entity? Case report and literature review. Pathol Res Pract. 2014;210:189-93 pubmed publisher
immunohistochemistry - paraffin section; human; 1:400; fig 1	Taylor N, Zighelboim I, Huettner P, Powell M, Gibb R, Rader J, et al. DNA mismatch repair and TP53 defects are early events in uterine carcinosarcoma tumorigenesis. Mod Pathol. 2006;19:1333-8 pubmed
immunohistochemistry - paraffin section; human; 1:200; fig 2	Tobón-Arroyave S, Flórez-Moreno G, Jaramillo-Cárdenas J, Arango-Uribe J, Isaza-Guzmán D, Rendón-Henao J. Expression of hMLH1 and hMSH2 proteins in pleomorphic adenoma of minor salivary glands: relationship with clinical and histologic findings. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:227-36 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Mei M, Deng D, Liu T, Sang X, Lu X, Xiang H, et al. Clinical implications of microsatellite instability and MLH1 gene inactivation in sporadic insulinomas. J Clin Endocrinol Metab. 2009;94:3448-57 pubmed publisher
immunohistochemistry; human; 1:20	Aust D, Haase M, Dobryden L, Markwarth A, Löhrs U, Wittekind C, et al. Mutations of the BRAF gene in ulcerative colitis-related colorectal carcinoma. Int J Cancer. 2005;115:673-7 pubmed
immunohistochemistry - paraffin section; human; 1:200; tbl 2	Coppola D, Nicosia S, Doty A, Sellers T, Lee J, Fulp J, et al. Uncertainty in the utility of immunohistochemistry in mismatch repair protein expression in epithelial ovarian cancer. Anticancer Res. 2012;32:4963-9 pubmed
immunohistochemistry - paraffin section; human; 1:400	Charara M, Edmonston T, Burkholder S, Walters R, Anne P, Mitchell E, et al. Microsatellite status and cell cycle associated markers in rectal cancer patients undergoing a combined regimen of 5-FU and CPT-11 chemotherapy and radiotherapy. Anticancer Res. 2004;24:3161-7 pubmed
immunohistochemistry - paraffin section; human; 1:100	Bendardaf R, Lamlum H, Ristamaki R, Korkeila E, Syrjanen K, Pyrhonen S. Thymidylate synthase and microsatellite instability in colorectal cancer: implications for disease free survival, treatment response and survival with metastases. Acta Oncol. 2008;47:1046-53 pubmed
	Kenyon J, Fu P, Lingas K, Thomas E, Saurastri A, Santos Guasch G, et al. Humans accumulate microsatellite instability with acquired loss of MLH1 protein in hematopoietic stem and progenitor cells as a function of age. Blood. 2012;120:3229-36 pubmed publisher
	Al-Temaimi R, Jacob S, Al-Ali W, Thomas D, Al-Mulla F. Reduced FHIT expression is associated with mismatch repair deficient and high CpG island methylator phenotype colorectal cancer. J Histochem Cytochem. 2013;61:627-38 pubmed publisher

product information

Product Type :

Antibody

Product Name :

MSH2 Monoclonal Antibody (FE11)

Catalog # :

33-7900

Quantity :

50µg

Price :

US 275.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 2 µg/ml, Immunofluorescence: 2 µg/ml, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: 2 µg/ml

Species :

Human, Mouse

Clone :

FE11

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world.

Immunogen :

C-terminal fragment of the human MSH2 protein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/ml, Immunofluorescence: 2 µg/ml, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: 2 µg/ml

Aliases :

COCA1, FCC1, HNPCC, HNPCC1, LCFS2, DNA mismatch repair protein Msh2, hMSH2

PubMed References :

14760069, 15509520, 15510606, 15704157, 15930362, 15958853, 16136384, 16803513, 16810312, 17943475, 17973265, 18202787, 18254781, 18949393, 19124477, 19275958, 19464205, 19567531, 19821155, 20227038, 21733559, 21937259, 22740444, 23066952, 23155266, 23194196, 23797051, 24205245, 24315830, 24432405, 24592941, 25710585, 25798424, 26714314, 26964739, 27816197

more info or order :

Invitrogen product webpage