PLK1 Antibody Cocktail | Invitrogen 33-1700 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PLK1 Antibody Cocktail

catalog :

33-1700

quantity :

100µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PL6/PL2

reactivity :

human, mouse, rat, common platanna

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, other

more info or order :

Invitrogen product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 4d	Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed publisher
western blot; human; 1:500; fig s1	Engel K, Rudelius M, Slawska J, Jacobs L, Ahangarian Abhari B, Altmann B, et al. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. EMBO Mol Med. 2016;8:851-62 pubmed publisher
immunoprecipitation; human; fig 4 western blot; human; fig 4	Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
other; human; loading ...; fig st1	Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
western blot; human; 1:500; fig 2	Kim J, Lee K, Rhee K. PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit. Nat Commun. 2015;6:10076 pubmed publisher
western blot; mouse; fig 1	Valianou M, Cox A, Pichette B, Hartley S, Paladhi U, Astrinidis A. Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy. Cell Cycle. 2015;14:399-407 pubmed publisher
western blot; human	van der Lelij P, Stocsits R, Ladurner R, Petzold G, Kreidl E, Koch B, et al. SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs. EMBO J. 2014;33:2643-58 pubmed publisher
immunoprecipitation; human; fig 2 western blot; human; fig 2	Ando K, Ozaki T, Hirota T, Nakagawara A. NFBD1/MDC1 is phosphorylated by PLK1 and controls G2/M transition through the regulation of a TOPOII?-mediated decatenation checkpoint. PLoS ONE. 2013;8:e82744 pubmed publisher
western blot; human; 1:500; fig 5	Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
western blot; human; fig 7	Ovejero S, Ayala P, Bueno A, Sacristán M. Human Cdc14A regulates Wee1 stability by counteracting CDK-mediated phosphorylation. Mol Biol Cell. 2012;23:4515-25 pubmed publisher
western blot; human; 1:300	Kubara P, Kernéis Golsteyn S, Studény A, Lanser B, Meijer L, Golsteyn R. Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents. Biochem J. 2012;446:373-81 pubmed publisher
immunocytochemistry; human; fig 1 western blot; human; fig 2	Hwang H, Ji J, Jang Y. Phosphorylation of Ran-binding protein-1 by Polo-like kinase-1 is required for interaction with Ran and early mitotic progression. J Biol Chem. 2011;286:33012-20 pubmed publisher
western blot; human; fig 1	Lee J, Kim J, Margolis R, Fotedar R. Substrate degradation by the anaphase promoting complex occurs during mitotic slippage. Cell Cycle. 2010;9:1792-801 pubmed
western blot; human; fig 1	Lee J, Kim J, Barbier V, Fotedar A, Fotedar R. DNA damage triggers p21WAF1-dependent Emi1 down-regulation that maintains G2 arrest. Mol Biol Cell. 2009;20:1891-902 pubmed publisher
immunoprecipitation; human; fig 2b	Wu Z, Yang X, Weber G, Liu X. Plk1 phosphorylation of TRF1 is essential for its binding to telomeres. J Biol Chem. 2008;283:25503-13 pubmed publisher
immunoprecipitation; human western blot; human	Soond S, Barry S, Melino G, Knight R, Latchman D, Stephanou A. p73-mediated transcriptional activity is negatively regulated by polo-like kinase 1. Cell Cycle. 2008;7:1214-23 pubmed
western blot; human; fig 2	Flatmark K, Nome R, Folkvord S, Bratland A, Rasmussen H, Ellefsen M, et al. Radiosensitization of colorectal carcinoma cell lines by histone deacetylase inhibition. Radiat Oncol. 2006;1:25 pubmed
western blot; human; fig 1B	Lyng H, Landsverk K, Kristiansen E, DeAngelis P, Ree A, Myklebost O, et al. Response of malignant B lymphocytes to ionizing radiation: gene expression and genotype. Int J Cancer. 2005;115:935-42 pubmed
immunocytochemistry; human; 5 ug/ml; fig 6 western blot; human; fig 2B	Hansen D, Loktev A, Ban K, Jackson P. Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1. Mol Biol Cell. 2004;15:5623-34 pubmed
western blot; human; fig 6	Crawford D, Piwnica Worms H. The G(2) DNA damage checkpoint delays expression of genes encoding mitotic regulators. J Biol Chem. 2001;276:37166-77 pubmed
	Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
	Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, et al. CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation. J Biol Chem. 2012;287:12975-84 pubmed publisher
	Hansen D, Tung J, Jackson P. CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. Proc Natl Acad Sci U S A. 2006;103:608-13 pubmed
	Kho P, Wang Z, Zhuang L, Li Y, Chew J, Ng H, et al. p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis. J Biol Chem. 2004;279:21183-92 pubmed
	Kraft C, Herzog F, Gieffers C, Mechtler K, Hagting A, Pines J, et al. Mitotic regulation of the human anaphase-promoting complex by phosphorylation. EMBO J. 2003;22:6598-609 pubmed

product information

Product Type :

Antibody

Product Name :

PLK1 Antibody Cocktail

Catalog # :

33-1700

Quantity :

100µg

Price :

US 309.00

Clonality :

Cocktail

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat, Xenopus laevis

Applications :

ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1-5 µg/mL, Immunofluorescence: 1-5 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 5 µg, Western Blot: 1-2 µg/mL

Species :

Human, Mouse, Non-human primate, Rat, Xenopus laevis

Clone :

PL6/PL2

Isotype :

IgG1, kappa/IgG2b

Storage :

-20°C

Description :

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Immunogen :

A His-tagged Plk fusion protein (C-term Plk1) containing amino acids 402-603 of the human Plk1 protein(

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 1-5 µg/mL, Immunofluorescence: 1-5 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 5 µg, Western Blot: 1-2 µg/mL

Aliases :

Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13, PLK1, PLK

more info or order :

Invitrogen product webpage