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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
alpha Tubulin Monoclonal Antibody (B-5-1-2)
catalog :
32-2500
quantity :
100µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B-5-1-2
reactivity :
human, mouse, rat, dog, baker's yeast, thale cress, rabbit
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 46
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 1a
Schauwecker S, Kim J, Licht J, Clevenger C. Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells. J Biol Chem. 2017;292:2237-2254 pubmed publisher
  • western blot; thale cress; loading ...; fig 1h
Mir R, Aranda L, Biaocchi T, Luo A, Sylvester A, Rasmussen C. A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1. Plant Physiol. 2017;173:863-871 pubmed publisher
  • western blot; human; 1:20,000; loading ...; fig 3b
Blanco F, Preet R, Aguado A, Vishwakarma V, Stevens L, Vyas A, et al. Impact of HuR inhibition by the small molecule MS-444 on colorectal cancer cell tumorigenesis. Oncotarget. 2016;7:74043-74058 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3a
Harrington K, Clevenger C. Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration. J Biol Chem. 2016;291:21388-21406 pubmed
  • western blot; human; 1:1000; loading ...; fig 1c
Medler T, Craig J, Fiorillo A, Feeney Y, Harrell J, Clevenger C. HDAC6 Deacetylates HMGN2 to Regulate Stat5a Activity and Breast Cancer Growth. Mol Cancer Res. 2016;14:994-1008 pubmed
  • western blot; rat; fig 1
Fortes M, Marzuca Nassr G, Vitzel K, da Justa Pinheiro C, Newsholme P, Curi R. Housekeeping proteins: How useful are they in skeletal muscle diabetes studies and muscle hypertrophy models?. Anal Biochem. 2016;504:38-40 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
  • western blot; human; 1:1000; fig 1
Guerrera D, Shah J, Vasileva E, Sluysmans S, Méan I, Jond L, et al. PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins. J Biol Chem. 2016;291:11016-29 pubmed publisher
  • western blot; human; fig 3
Sakakini N, Turchi L, Bergon A, Holota H, Rekima S, Lopez F, et al. A Positive Feed-forward Loop Associating EGR1 and PDGFA Promotes Proliferation and Self-renewal in Glioblastoma Stem Cells. J Biol Chem. 2016;291:10684-99 pubmed publisher
  • western blot; mouse; fig s4
Graindorge A, Frénal K, Jacot D, Salamun J, Marq J, Soldati Favre D. The Conoid Associated Motor MyoH Is Indispensable for Toxoplasma gondii Entry and Exit from Host Cells. PLoS Pathog. 2016;12:e1005388 pubmed publisher
  • western blot; mouse; 1:2000; fig 1c
Ye S, Zhang D, Cheng F, Wilson D, Mackay J, He K, et al. Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal. J Cell Sci. 2016;129:269-76 pubmed publisher
  • western blot; human; 1:10,000; fig 4c
Blanco F, Jimbo M, Wulfkuhle J, Gallagher I, Deng J, Enyenihi L, et al. The mRNA-binding protein HuR promotes hypoxia-induced chemoresistance through posttranscriptional regulation of the proto-oncogene PIM1 in pancreatic cancer cells. Oncogene. 2016;35:2529-41 pubmed publisher
  • western blot; mouse; 1:2000
Qiu D, Ye S, Ruiz B, Zhou X, Liu D, Zhang Q, et al. Klf2 and Tfcp2l1, Two Wnt/β-Catenin Targets, Act Synergistically to Induce and Maintain Naive Pluripotency. Stem Cell Reports. 2015;5:314-22 pubmed publisher
  • western blot; human; 1:10,000; fig 1
Jimbo M, Blanco F, Huang Y, Telonis A, Screnci B, Cosma G, et al. Targeting the mRNA-binding protein HuR impairs malignant characteristics of pancreatic ductal adenocarcinoma cells. Oncotarget. 2015;6:27312-31 pubmed publisher
  • immunocytochemistry; baker's yeast; 1:200
Silva R, Dautel M, Di Genova B, Amberg D, Castilho B, Sattlegger E. The Gcn2 Regulator Yih1 Interacts with the Cyclin Dependent Kinase Cdc28 and Promotes Cell Cycle Progression through G2/M in Budding Yeast. PLoS ONE. 2015;10:e0131070 pubmed publisher
  • western blot; rat
Akkuratov E, Lopacheva O, Kruusmägi M, Lopachev A, Shah Z, Boldyrev A, et al. Functional Interaction Between Na/K-ATPase and NMDA Receptor in Cerebellar Neurons. Mol Neurobiol. 2015;52:1726-1734 pubmed publisher
  • western blot; human
Pereira L, Pinto R, Silva D, Moreira A, Beitzinger C, Oliveira P, et al. Intracellular trafficking of AIP56, an NF-κB-cleaving toxin from Photobacterium damselae subsp. piscicida. Infect Immun. 2014;82:5270-85 pubmed publisher
  • western blot; human; 1:500
Bastos L, de Marcondes P, de Freitas Junior J, Leve F, Mencalha A, de Souza W, et al. Progeny from irradiated colorectal cancer cells acquire an EMT-like phenotype and activate Wnt/?-catenin pathway. J Cell Biochem. 2014;115:2175-87 pubmed publisher
  • immunocytochemistry; dog; 1:150; fig 1c
  • western blot; dog; 1:1000; fig 1b
Paschoud S, Jond L, Guerrera D, Citi S. PLEKHA7 modulates epithelial tight junction barrier function. Tissue Barriers. 2014;2:e28755 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Yamano K, Fogel A, Wang C, van der Bliek A, Youle R. Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy. elife. 2014;3:e01612 pubmed publisher
  • western blot; human; 2 ug/ml; fig 3
Song X, Chen H, Wang X, Deng X, Xi Y, He Q, et al. H. pylori-encoded CagA disrupts tight junctions and induces invasiveness of AGS gastric carcinoma cells via Cdx2-dependent targeting of Claudin-2. Cell Immunol. 2013;286:22-30 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:2000; fig 4
Leonard A, Manavis J, Blumbergs P, Vink R. Changes in substance P and NK1 receptor immunohistochemistry following human spinal cord injury. Spinal Cord. 2014;52:17-23 pubmed publisher
  • western blot; human; fig 1
Yamano K, Youle R. PINK1 is degraded through the N-end rule pathway. Autophagy. 2013;9:1758-69 pubmed publisher
  • western blot; human; 1:1000; fig 1
de Souza W, Fortunato Miranda N, Robbs B, de Araujo W, de Freitas Junior J, Bastos L, et al. Claudin-3 overexpression increases the malignant potential of colorectal cancer cells: roles of ERK1/2 and PI3K-Akt as modulators of EGFR signaling. PLoS ONE. 2013;8:e74994 pubmed publisher
  • western blot; human; fig 1
Toth J, Yang L, Dahl R, Petroski M. A gatekeeper residue for NEDD8-activating enzyme inhibition by MLN4924. Cell Rep. 2012;1:309-16 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig s1
  • immunohistochemistry - paraffin section; human; 1:200; fig s1
Gerbe F, van Es J, Makrini L, Brulin B, Mellitzer G, Robine S, et al. Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. J Cell Biol. 2011;192:767-80 pubmed publisher
  • immunocytochemistry; dog; 1:100; fig 4
Paschoud S, Yu D, Pulimeno P, Jond L, Turner J, Citi S. Cingulin and paracingulin show similar dynamic behaviour, but are recruited independently to junctions. Mol Membr Biol. 2011;28:123-35 pubmed publisher
  • immunocytochemistry; human; 2.5 mg/ml; fig 1
Soenen S, Himmelreich U, Nuytten N, De Cuyper M. Cytotoxic effects of iron oxide nanoparticles and implications for safety in cell labelling. Biomaterials. 2011;32:195-205 pubmed publisher
  • western blot; human; fig 3
Warters R, Cassidy P, Sunseri J, Parsawar K, Zhuplatov S, Kramer G, et al. The nuclear matrix shell proteome of human epidermis. J Dermatol Sci. 2010;58:113-22 pubmed publisher
  • western blot; human; fig 2
Fang F, Zheng J, Galbaugh T, Fiorillo A, Hjort E, Zeng X, et al. Cyclophilin B as a co-regulator of prolactin-induced gene expression and function in breast cancer cells. J Mol Endocrinol. 2010;44:319-29 pubmed publisher
  • immunocytochemistry; human
Frescas D, Guardavaccaro D, Kuchay S, Kato H, Poleshko A, Basrur V, et al. KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state. Cell Cycle. 2008;7:3539-47 pubmed
  • western blot; human; fig s4
Guardavaccaro D, Frescas D, Dorrello N, Peschiaroli A, Multani A, Cardozo T, et al. Control of chromosome stability by the beta-TrCP-REST-Mad2 axis. Nature. 2008;452:365-9 pubmed publisher
  • western blot; rabbit; fig 1
Akoyev V, Takemoto D. ZO-1 is required for protein kinase C gamma-driven disassembly of connexin 43. Cell Signal. 2007;19:958-67 pubmed
  • western blot; mouse; 1:1000; fig 3
Hough S, Clements I, Welch P, Wiederholt K. Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog. Stem Cells. 2006;24:1467-75 pubmed
  • immunocytochemistry; human; 1:200; fig 1
Akileswaran L, Taraska J, Sayer J, Gettemy J, Coghlan V. A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase. J Biol Chem. 2001;276:17448-54 pubmed
  • immunocytochemistry; human; 1:100
Ashton A, Yokota R, John G, Zhao S, Suadicani S, Spray D, et al. Inhibition of endothelial cell migration, intercellular communication, and vascular tube formation by thromboxane A(2). J Biol Chem. 1999;274:35562-70 pubmed
Chen X, Wang R, Liu X, Wu Y, Zhou T, Yang Y, et al. A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3? and GSK3? in Regulating Embryonic Stem Cell Fate. Dev Cell. 2017;43:563-576.e4 pubmed publisher
Zarei M, Lal S, Parker S, Nevler A, Vaziri Gohar A, Dukleska K, et al. Posttranscriptional Upregulation of IDH1 by HuR Establishes a Powerful Survival Phenotype in Pancreatic Cancer Cells. Cancer Res. 2017;77:4460-4471 pubmed publisher
Ye S, Zhang T, Tong C, Zhou X, He K, Ban Q, et al. Depletion of Tcf3 and Lef1 maintains mouse embryonic stem cell self-renewal. Biol Open. 2017;6:511-517 pubmed publisher
Fu Y, Winter P, Rojas R, Wang V, McAuliffe M, Patterson G. Axial superresolution via multiangle TIRF microscopy with sequential imaging and photobleaching. Proc Natl Acad Sci U S A. 2016;113:4368-73 pubmed publisher
Jin S, Youle R. The accumulation of misfolded proteins in the mitochondrial matrix is sensed by PINK1 to induce PARK2/Parkin-mediated mitophagy of polarized mitochondria. Autophagy. 2013;9:1750-7 pubmed publisher
Ye S, Li P, Tong C, Ying Q. Embryonic stem cell self-renewal pathways converge on the transcription factor Tfcp2l1. EMBO J. 2013;32:2548-60 pubmed publisher
Peng S, Gan G, Rao V, Adelman R, Rizzolo L. Effects of proinflammatory cytokines on the claudin-19 rich tight junctions of human retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2012;53:5016-28 pubmed publisher
Sanchez Adams J, Athanasiou K. Biomechanics of meniscus cells: regional variation and comparison to articular chondrocytes and ligament cells. Biomech Model Mechanobiol. 2012;11:1047-56 pubmed publisher
Fang F, Flegler A, Du P, Lin S, Clevenger C. Expression of cyclophilin B is associated with malignant progression and regulation of genes implicated in the pathogenesis of breast cancer. Am J Pathol. 2009;174:297-308 pubmed publisher
Guo Y, Martinez Williams C, Rannels D. Gap junction-microtubule associations in rat alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1213-21 pubmed
Korfali N, Ruchaud S, Loegering D, Bernard D, Dingwall C, Kaufmann S, et al. Caspase-7 gene disruption reveals an involvement of the enzyme during the early stages of apoptosis. J Biol Chem. 2004;279:1030-9 pubmed
product information
Product Type :
Antibody
Product Name :
alpha Tubulin Monoclonal Antibody (B-5-1-2)
Catalog # :
32-2500
Quantity :
100µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Canine, Human, Mouse, Non-human primate, Rat
Applications :
Immunocytochemistry: 5-10 µg/ml, Immunofluorescence: 5-10 ug/ml, Western Blot: 1 µg/ml
Species :
Canine, Human, Mouse, Non-human primate, Rat
Clone :
B-5-1-2
Isotype :
IgG1
Storage :
-20°C
Description :
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species.
Immunogen :
Sarkosyl-resistant filament from sea urchin sperm axonemes.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5-10 µg/ml, Immunofluorescence: 5-10 ug/ml, Western Blot: 1 µg/ml
Aliases :
FLJ30169, H2 ALPHA, Testis specific alpha tubulin, TUBA1, Tubulin alpha
PubMed References :
10585431, 11279182, 14583630, 14604851, 16456133, 17210245, 18354482, 19001877, 19056847, 20237142, 20363599, 20863560, 21166484, 21383077, 22231673, 22761260, 22832224, 23942238, 24069372, 24121706, 24149988, 24216617, 24287273, 24569479, 24843844, 25103643, 25287919, 25381029, 26176233, 26314962, 26321140, 26387536, 26598557, 26760042, 27002148, 27044745, 27060530, 27489110, 27677075, 27881728, 28035005, 28288968
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA