Tau Monoclonal Antibody (T46) | Invitrogen 13-6400 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Tau Monoclonal Antibody (T46)

catalog :

13-6400

quantity :

100µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

T46

reactivity :

human, mouse, rat, chicken, cow, rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:1000; loading ...; fig 1b	Hwang A, Trzeciakiewicz H, Friedmann D, Yuan C, Marmorstein R, Lee V, et al. Conserved Lysine Acetylation within the Microtubule-Binding Domain Regulates MAP2/Tau Family Members. PLoS ONE. 2016;11:e0168913 pubmed publisher
immunoprecipitation; human western blot; human; 1:1000; fig 1b	Trzeciakiewicz H, Tseng J, Wander C, Madden V, Tripathy A, Yuan C, et al. A Dual Pathogenic Mechanism Links Tau Acetylation to Sporadic Tauopathy. Sci Rep. 2017;7:44102 pubmed publisher
immunocytochemistry; rat; 1:100; loading ...; fig 4b	Atasoy İ, Dursun E, Gezen Ak D, Metin Armağan D, Ozturk M, Yilmazer S. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. J Chem Neuroanat. 2017;80:19-26 pubmed publisher
immunocytochemistry; human; 1:200; fig 2c western blot; human; 1:1000; fig 2e	LÃ³pez de Maturana R, Lang V, Zubiarrain A, Sousa A, VÃ¡zquez N, Gorostidi A, et al. Mutations in LRRK2 impair NF-ÎºB pathway in iPSC-derived neurons. J Neuroinflammation. 2016;13:295 pubmed
western blot; mouse; 1:1000; fig 7	Peng Y, Kim M, Hullinger R, O Riordan K, Burger C, Pehar M, et al. Improved proteostasis in the secretory pathway rescues Alzheimer's disease in the mouse. Brain. 2016;139:937-52 pubmed publisher
immunohistochemistry; human; 1:500; fig 6 western blot; human; fig 2	Taniguchi Watanabe S, Arai T, Kametani F, Nonaka T, Masuda Suzukake M, Tarutani A, et al. Biochemical classification of tauopathies by immunoblot, protein sequence and mass spectrometric analyses of sarkosyl-insoluble and trypsin-resistant tau. Acta Neuropathol. 2016;131:267-280 pubmed publisher
western blot; human; 1:1000	Takeuchi R, Toyoshima Y, Tada M, Tanaka H, Shimizu H, Shiga A, et al. Globular Glial Mixed Four Repeat Tau and TDP-43 Proteinopathy with Motor Neuron Disease and Frontotemporal Dementia. Brain Pathol. 2016;26:82-94 pubmed publisher
western blot; human	Corbel C, Zhang B, Le Parc A, Baratte B, Colas P, Couturier C, et al. Tamoxifen inhibits CDK5 kinase activity by interacting with p35/p25 and modulates the pattern of tau phosphorylation. Chem Biol. 2015;22:472-482 pubmed publisher
western blot; human; fig 1	Melis V, Zabke C, Stamer K, Magbagbeolu M, Schwab K, Marschall P, et al. Different pathways of molecular pathophysiology underlie cognitive and motor tauopathy phenotypes in transgenic models for Alzheimer's disease and frontotemporal lobar degeneration. Cell Mol Life Sci. 2015;72:2199-222 pubmed publisher
	Zhou Y, Shi J, Chu D, Hu W, Guan Z, Gong C, et al. Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease. Front Aging Neurosci. 2018;10:27 pubmed publisher
	Wang B, Tanaka K, Ji B, Ono M, Fang Y, Ninomiya Y, et al. Total body 100-mGy X-irradiation does not induce Alzheimer's disease-like pathogenesis or memory impairment in mice. J Radiat Res. 2014;55:84-96 pubmed publisher
	Guo J, Arai T, Miklossy J, McGeer P. Abeta and tau form soluble complexes that may promote self aggregation of both into the insoluble forms observed in Alzheimer's disease. Proc Natl Acad Sci U S A. 2006;103:1953-8 pubmed
	Mendes S, Henkemeyer M, Liebl D. Multiple Eph receptors and B-class ephrins regulate midline crossing of corpus callosum fibers in the developing mouse forebrain. J Neurosci. 2006;26:882-92 pubmed
	Yu W, Ko M, Yanagisawa K, Michikawa M. Neurodegeneration in heterozygous Niemann-Pick type C1 (NPC1) mouse: implication of heterozygous NPC1 mutations being a risk for tauopathy. J Biol Chem. 2005;280:27296-302 pubmed
	Arai T, Guo J, McGeer P. Proteolysis of non-phosphorylated and phosphorylated tau by thrombin. J Biol Chem. 2005;280:5145-53 pubmed
	Zamora Leon S, Bresnick A, Backer J, Shafit Zagardo B. Fyn phosphorylates human MAP-2c on tyrosine 67. J Biol Chem. 2005;280:1962-70 pubmed
	Rissman R, Poon W, Blurton Jones M, Oddo S, Torp R, Vitek M, et al. Caspase-cleavage of tau is an early event in Alzheimer disease tangle pathology. J Clin Invest. 2004;114:121-30 pubmed
	Clark L, Poorkaj P, Wszolek Z, Geschwind D, Nasreddine Z, Miller B, et al. Pathogenic implications of mutations in the tau gene in pallido-ponto-nigral degeneration and related neurodegenerative disorders linked to chromosome 17. Proc Natl Acad Sci U S A. 1998;95:13103-7 pubmed
	Reed L, Schmidt M, Wszolek Z, Balin B, Soontornniyomkij V, Lee V, et al. The neuropathology of a chromosome 17-linked autosomal dominant parkinsonism and dementia ("pallido-ponto-nigral degeneration"). J Neuropathol Exp Neurol. 1998;57:588-601 pubmed
	Lieberman A, Trojanowski J, Lee V, Balin B, Ding X, Greenberg J, et al. Cognitive, neuroimaging, and pathological studies in a patient with Pick's disease. Ann Neurol. 1998;43:259-65 pubmed

product information

Product Type :

Antibody

Product Name :

Tau Monoclonal Antibody (T46)

Catalog # :

13-6400

Quantity :

100µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Bovine, Chicken, Human, Mouse, Rabbit, Rat, Xenopus laevis

Applications :

ELISA: 0.1-0.5 µg/mL, Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunohistochemistry: 5-25 µg/mL, Immunomicroscopy: Assay Dependent, Immunoprecipitation: 2-5 µg, Western Blot: 0.5-1.0 µg/mL

Species :

Bovine, Chicken, Human, Mouse, Rabbit, Rat, Xenopus laevis

Clone :

T46

Isotype :

IgG1

Storage :

-20°C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclindependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

Bovine Tau.

Format :

Liquid

Applications w/Dilutions :

Aliases :

MAPT, Microtubule-associated protein tau, MTBT1, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

more info or order :

Invitrogen product webpage