product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ERK1/ERK2 Monoclonal Antibody (ERK-7D8)
catalog :
13-6200
quantity :
200µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ERK-7D8
reactivity :
human, mouse, rat, dog, common platanna
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 35
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 5a
Zhang C, Mao H, Cao Y. Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner. Sci Rep. 2017;7:3769 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 3a
Yan X, Zhu Z, Xu S, Yang L, Liao X, Zheng M, et al. MicroRNA-140-5p inhibits hepatocellular carcinoma by directly targeting the unique isomerase Pin1 to block multiple cancer-driving pathways. Sci Rep. 2017;7:45915 pubmed publisher
  • western blot; human; fig 5e
Grugan K, Dorn K, Jarantow S, Bushey B, Pardinas J, Laquerre S, et al. Fc-mediated activity of EGFR x c-Met bispecific antibody JNJ-61186372 enhanced killing of lung cancer cells. MAbs. 2017;9:114-126 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 2a
Aguilera O, Muñoz Sagastibelza M, Torrejón B, Borrero Palacios A, del Puerto Nevado L, Martínez Useros J, et al. Vitamin C uncouples the Warburg metabolic switch in KRAS mutant colon cancer. Oncotarget. 2016;7:47954-47965 pubmed publisher
  • western blot; human; fig 1
Salas E, Roy S, Marsh T, Rubin B, Debnath J. Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell death. Oncogene. 2016;35:2913-22 pubmed publisher
  • western blot; mouse; 1:500; fig 5b
Murrow L, Malhotra R, Debnath J. ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function. Nat Cell Biol. 2015;17:300-10 pubmed publisher
  • western blot; human
Bernusso V, Machado Neto J, Pericole F, Vieira K, Duarte A, Traina F, et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta. 2015;1853:388-95 pubmed publisher
  • western blot; mouse
Janardhan S, Marks R, Gajewski T. Primary murine CD4+ T cells fail to acquire the ability to produce effector cytokines when active Ras is present during Th1/Th2 differentiation. PLoS ONE. 2014;9:e112831 pubmed publisher
  • western blot; human
Li L, Wu P, Lee J, Li P, Hsieh W, Ho C, et al. Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells. PLoS ONE. 2014;9:e104203 pubmed publisher
  • western blot; rat; 1:2500; tbl 1
Iio W, Matsukawa N, Tsukahara T, Toyoda A. The effects of oral taurine administration on behavior and hippocampal signal transduction in rats. Amino Acids. 2012;43:2037-46 pubmed publisher
  • western blot; human
Machado Neto J, Favaro P, Lazarini M, Costa F, Olalla Saad S, Traina F. Knockdown of insulin receptor substrate 1 reduces proliferation and downregulates Akt/mTOR and MAPK pathways in K562 cells. Biochim Biophys Acta. 2011;1813:1404-11 pubmed publisher
  • western blot; human; fig 1
Urbanet R, Pilon C, Giorgino F, Vettor R, Fallo F. Insulin signaling in adipose tissue of patients with primary aldosteronism. J Endocrinol Invest. 2011;34:86-9 pubmed
  • western blot; mouse; fig 2a
Zha Y, Marks R, Ho A, Peterson A, Janardhan S, Brown I, et al. T cell anergy is reversed by active Ras and is regulated by diacylglycerol kinase-alpha. Nat Immunol. 2006;7:1166-73 pubmed
  • western blot; rat; fig 6
Zhu M, Feng J, Lucchinetti E, Fischer G, Xu L, Pedrazzini T, et al. Ischemic postconditioning protects remodeled myocardium via the PI3K-PKB/Akt reperfusion injury salvage kinase pathway. Cardiovasc Res. 2006;72:152-62 pubmed
  • western blot; rat; fig 7
Lo I, Shih J, Jiang M. Reactive oxygen species and ERK 1/2 mediate monocyte chemotactic protein-1-stimulated smooth muscle cell migration. J Biomed Sci. 2005;12:377-88 pubmed
  • western blot; rat; 1:5000; fig 2
Tsai M, Jiang M. Extracellular signal-regulated kinase1/2 in contraction of vascular smooth muscle. Life Sci. 2005;76:877-88 pubmed
  • western blot; rat; fig 5
Usui S, Sugimoto N, Takuwa N, Sakagami S, Takata S, Kaneko S, et al. Blood lipid mediator sphingosine 1-phosphate potently stimulates platelet-derived growth factor-A and -B chain expression through S1P1-Gi-Ras-MAPK-dependent induction of Kruppel-like factor 5. J Biol Chem. 2004;279:12300-11 pubmed
  • western blot; human; fig 2
Hernandez M, Barrero M, Crespo M, Nieto M. Lysophosphatidic acid inhibits Ca2+ signaling in response to epidermal growth factor receptor stimulation in human astrocytoma cells by a mechanism involving phospholipase C(gamma) and a G(alphai) protein. J Neurochem. 2000;75:1575-82 pubmed
  • western blot; rat
Chan E, Stang S, Bottorff D, Stone J. Hypothermic stress leads to activation of Ras-Erk signaling. J Clin Invest. 1999;103:1337-44 pubmed
  • western blot; mouse; fig 1, 2
Korneyev A. Stress-induced tau phosphorylation in mouse strains with different brain Erk 1 + 2 immunoreactivity. Neurochem Res. 1998;23:1539-43 pubmed
  • immunoprecipitation; mouse
  • western blot; mouse
Webb C, Van Aelst L, Wigler M, Vande Woude G. Signaling pathways in Ras-mediated tumorigenicity and metastasis. Proc Natl Acad Sci U S A. 1998;95:8773-8 pubmed
  • western blot; common platanna; 1:1000; fig 1
Duesbery N, Webb C, Leppla S, Gordon V, Klimpel K, Copeland T, et al. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science. 1998;280:734-7 pubmed
  • western blot; human; fig 3
Hidari K, Weyrich A, Zimmerman G, McEver R. Engagement of P-selectin glycoprotein ligand-1 enhances tyrosine phosphorylation and activates mitogen-activated protein kinases in human neutrophils. J Biol Chem. 1997;272:28750-6 pubmed
Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher
Mòdol L, Mancuso R, Alé A, Francos Quijorna I, Navarro X. Differential effects on KCC2 expression and spasticity of ALS and traumatic injuries to motoneurons. Front Cell Neurosci. 2014;8:7 pubmed publisher
Montenegro L, Leal A, Coutinho D, Valassi H, Nishi M, Arnhold I, et al. Post-receptor IGF1 insensitivity restricted to the MAPK pathway in a Silver-Russell syndrome patient with hypomethylation at the imprinting control region on chromosome 11. Eur J Endocrinol. 2012;166:543-50 pubmed publisher
Janardhan S, Praveen K, Marks R, Gajewski T. Evidence implicating the Ras pathway in multiple CD28 costimulatory functions in CD4+ T cells. PLoS ONE. 2011;6:e24931 pubmed publisher
Driessens G, Zheng Y, Locke F, Cannon J, Gounari F, Gajewski T. Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-?1 phosphorylation. J Immunol. 2011;186:784-90 pubmed publisher
Yin G, Zheng Q, Yan C, Berk B. GIT1 is a scaffold for ERK1/2 activation in focal adhesions. J Biol Chem. 2005;280:27705-12 pubmed
Michlig S, Mercier A, Doucet A, Schild L, Horisberger J, Rossier B, et al. ERK1/2 controls Na,K-ATPase activity and transepithelial sodium transport in the principal cell of the cortical collecting duct of the mouse kidney. J Biol Chem. 2004;279:51002-12 pubmed
Natalicchio A, Laviola L, De Tullio C, Renna L, Montrone C, Perrini S, et al. Role of the p66Shc isoform in insulin-like growth factor I receptor signaling through MEK/Erk and regulation of actin cytoskeleton in rat myoblasts. J Biol Chem. 2004;279:43900-9 pubmed
Martinez Quiles N, Ho H, Kirschner M, Ramesh N, Geha R. Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP. Mol Cell Biol. 2004;24:5269-80 pubmed
Derkinderen P, Valjent E, Toutant M, Corvol J, Enslen H, Ledent C, et al. Regulation of extracellular signal-regulated kinase by cannabinoids in hippocampus. J Neurosci. 2003;23:2371-82 pubmed
Carraway C, Carvajal M, Carraway K. Association of the Ras to mitogen-activated protein kinase signal transduction pathway with microfilaments. Evidence for a p185(neu)-containing cell surface signal transduction particle linking the mitogenic pathway to a membrane-microfilament associ. J Biol Chem. 1999;274:25659-67 pubmed
Bird T, Sleath P, DeRoos P, Dower S, Virca G. Interleukin-1 represents a new modality for the activation of extracellular signal-regulated kinases/microtubule-associated protein-2 kinases. J Biol Chem. 1991;266:22661-70 pubmed
product information
Product Type :
Antibody
Product Name :
ERK1/ERK2 Monoclonal Antibody (ERK-7D8)
Catalog # :
13-6200
Quantity :
200µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Canine, Human, Mouse, Rat
Applications :
ELISA: 0.1-1 mg/mL, Gel Shift: Assay Dependent, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 2-5 µg, Western Blot: 1 µg/mL
Species :
Canine, Human, Mouse, Rat
Clone :
ERK-7D8
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on specific threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identified and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family are the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identified including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggest that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins including: RSK, c-Fos, c-Jun, c-Myc, c-Raf, MAP2, and MEK. MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by many factors. Erk2 is also known as microtubule-associated protein-2 kinase MAP kinase 2, p42-MAPK, or EGF receptor T669 (ERT1). Mek2 activates Erk2 by phosphorylation of neighboring threonine-183 and tyrosine-185 residues. Erk2, a serine/threonine kinase, phosphorylates microtubule associated protein-2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters, and hormones at the cell surface to the transcriptional events in the nucleus.
Immunogen :
Synthetic peptide (aa 324-345) corresponding to a segment near the C-terminus of rat ERK1
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1 mg/mL, Gel Shift: Assay Dependent, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: 2-5 µg, Western Blot: 1 µg/mL
Aliases :
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, MAPK, Mitogen activated protein kinase, ERK1, p42, Mitogen activated protein kinase 1, Mitogen activated protein kinase 2, p42 MAP Kinase, p44 MAP Kinase
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA