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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
STAT5 alpha Monoclonal Antibody (ST5a-2H2)
catalog :
13-3600
quantity :
100µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ST5a-2H2
reactivity :
African green monkey, human, mouse, rat, cow
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
more info or order :
Invitrogen product webpage
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - free floating section; rat; 1:100
Moravcová S, Červená K, Pačesová D, Bendová Z. Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide. J Neurosci Res. 2016;94:99-108 pubmed publisher
  • EMSA; cow; fig 6
Nguyen M, Boutinaud M, Pétridou B, Gabory A, Pannetier M, Chat S, et al. DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking. PLoS ONE. 2014;9:e111556 pubmed publisher
  • western blot; human; fig 5
Elsarraj H, Hong Y, Valdez K, Carletti M, Salah S, Raimo M, et al. A novel role of microRNA146b in promoting mammary alveolar progenitor cell maintenance. J Cell Sci. 2013;126:2446-58 pubmed publisher
  • western blot; mouse; fig 5
Hoff P, Rakow A, Gaber T, Hahne M, Sentürk U, Strehl C, et al. Preoperative irradiation for the prevention of heterotopic ossification induces local inflammation in humans. Bone. 2013;55:93-101 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:3000; fig 3
  • western blot; mouse; 1:500; fig 4
Yip S, Eguchi R, Grattan D, Bunn S. Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a. J Neuroendocrinol. 2012;24:1484-91 pubmed publisher
  • western blot; human; fig 2
Barros P, Lam E, Jordan P, Matos P. Rac1 signalling modulates a STAT5/BCL-6 transcriptional switch on cell-cycle-associated target gene promoters. Nucleic Acids Res. 2012;40:7776-87 pubmed publisher
  • western blot; mouse; 1:1000; fig 7
Zhao H, Pearson E, Brooks D, Coon J, Chen D, Demura M, et al. A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice. Endocrinology. 2012;153:2701-13 pubmed publisher
  • chromatin immunoprecipitation; human; fig 6
Kabotyanski E, Huetter M, Xian W, Rijnkels M, Rosen J. Integration of prolactin and glucocorticoid signaling at the beta-casein promoter and enhancer by ordered recruitment of specific transcription factors and chromatin modifiers. Mol Endocrinol. 2006;20:2355-68 pubmed
  • immunoprecipitation; African green monkey; fig 4
  • western blot; African green monkey; fig 4
Aittomaki S, Pesu M, Groner B, Janne O, Palvimo J, Silvennoinen O. Cooperation among Stat1, glucocorticoid receptor, and PU.1 in transcriptional activation of the high-affinity Fc gamma receptor I in monocytes. J Immunol. 2000;164:5689-97 pubmed
Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher
Ushiki T, Huntington N, Glaser S, Kiu H, Georgiou A, Zhang J, et al. Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells. PLoS ONE. 2016;11:e0162111 pubmed publisher
Silver D, Naora H, Liu J, Cheng W, Montell D. Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility. Cancer Res. 2004;64:3550-8 pubmed
Gewinner C, Hart G, Zachara N, Cole R, Beisenherz Huss C, Groner B. The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5. J Biol Chem. 2004;279:3563-72 pubmed
Chong M, Cornish A, Darwiche R, Stanley E, Purton J, Godfrey D, et al. Suppressor of cytokine signaling-1 is a critical regulator of interleukin-7-dependent CD8+ T cell differentiation. Immunity. 2003;18:475-87 pubmed
Saharinen P, Vihinen M, Silvennoinen O. Autoinhibition of Jak2 tyrosine kinase is dependent on specific regions in its pseudokinase domain. Mol Biol Cell. 2003;14:1448-59 pubmed
Cornish A, Chong M, Davey G, Darwiche R, Nicola N, Hilton D, et al. Suppressor of cytokine signaling-1 regulates signaling in response to interleukin-2 and other gamma c-dependent cytokines in peripheral T cells. J Biol Chem. 2003;278:22755-61 pubmed
Xu M, Nie L, Kim S, Sun X. STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta. EMBO J. 2003;22:893-904 pubmed
product information
Product Type :
Antibody
Product Name :
STAT5 alpha Monoclonal Antibody (ST5a-2H2)
Catalog # :
13-3600
Quantity :
100µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
ELISA: 0.1-1 µg/mL, Gel Shift: 1-3 µg, Immunocytochemistry: 1-3 µg/mL, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: 5 µg, Western Blot: 1-3 µg/mL
Species :
Human, Mouse, Rat
Clone :
ST5a-2H2
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
STATs (signal transducers and activators of transcription) were originally identified as two novel DNA-binding proteins (STAT1 and STAT2), which were found to play essential roles in interferon IFN-alpha- and IFN-gamma-regulated gene expression. Following the identification of these first two family members, five additional mammalian STAT proteins were cloned, and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6. All STAT proteins share several conserved structural and functional domains. In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, specific STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding, which results in the transcriptional activation of distinct target genes. At least one and oftentimes several STAT proteins are activated in response to all cytokines which utilize cytokine receptor superfamily members. Nevertheless, there is a striking specificity of STAT activation is seen in response to individual cytokines. STAT5 or MGF (mammary growth factor) was originally isolated by purification of tyrosine phosphorylated DNA binding proteins from prolactin-stimulated mammary tissue and from IL-3-stimulated myeloid cells. Subsequently, two highly related but distinct Stat5 genes (Stat5a and Stat5b) were identified in mouse. The amino acid sequences of STAT5a and 5b show ~ 96% sequence similarity, and both proteins are co-expressed in most tissues of both virgin and lactating mice. However, differential accumulation of 5a and 5b mRNAs have been reported for both muscle and mammary tissue. STAT5 has been reported to be activated by multiple cytokines including IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GMCSF, TPO, EPO, GH, and Prolactin (PRL). Both the STAT5a and 5b proteins recognize the GAS (gamma interferoin activating sequence) site TTCNNNGAA, and the preferred DNA-binding consensus site has been identified as TTCC(A>T)GGAA. Western blot analysis of the full-length STAT5a protein isolated from a variety of cultured cells, indicates that the protein migrates with an apparent molecular weight of ~95 kDa. C-terminal truncated forms of the STAT5a protein have also been reported. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the anti-apoptotic function of this gene in cells.
Immunogen :
A peptide corresponding to the C-terminus of murine Stat5a coupled to a protein carrier.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1 µg/mL, Gel Shift: 1-3 µg, Immunocytochemistry: 1-3 µg/mL, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: 5 µg, Western Blot: 1-3 µg/mL
Aliases :
Signal transducer and activator of transcription 5A, STAT5A, STAT5
PubMed References :
10820245, 12574125, 12665516, 12686600, 12705851, 14597631, 15150111, 16772529, 22508516, 22723377, 22775396, 23571050, 23572509, 25369064, 26420542
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA