DNA-PKcs (phospho Thr2609) antibody | GeneTex GTX24194 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

DNA-PKcs (phospho Thr2609) antibody

catalog :

GTX24194

quantity :

50.00μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

product information

Catalog No :

GTX24194

Product Name :

DNA-PKcs (phospho Thr2609) antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Product Type :

Primary antibody

Size :

50.00μg

list of Pubmed id :

21912620,22619307,22561027

Isotype :

IgG

Product Description :

Rabbit polyclonal antibody to DNA-PKcs (phospho Thr2609)

CrossReactivity :

Background :

DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.

Immunogen :

Synthetic peptide - amino acids 2604-2614 of human DNA PKcs - phosphorylated at the pT2609 residue

Specificity :

This phospho specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal by ELISA.This antibody recognises phospho T2609 DNA PKcs as demonstrated: IPed DNA PKcs (using another DNA PKcs antibody) blotted with the pT2609 antibody shows an inducible signal at the correct height after DNA damage.However, unfortunately the antibody picks up an IR-inducible signal in cells lacking DNA-PKcs (MO59J cells) at the same size as DNA-PKcs. We believe that this is 53BP1 which runs at the same size as DNA-PKcs and is also phosphorylated in an IR-dependent manner. Similar results were seen with another phospho DNA-PKcs antibody indicating that this result is general to antibodies to this phospho site, rather than specific to this antibody.This antibody may be a useful marker for DNA damage. It may also still be useful in the study of phospho DNA PKcs T2609.

Buffer :

0.02 M Potassium Phosphate, 0.15 M sodium chloride, pH 7.2 containing 0.01% sodium azide

Uses :

ELISA, ICC/IF, WB

Concentration :

1.1mg/ml

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.