MCM7 antibody [N2C2], Internal | GeneTex GTX110278 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

MCM7 antibody [N2C2], Internal

catalog :

GTX110278

quantity :

100.00μl

price :

299 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

product information

Catalog No :

GTX110278

Product Name :

MCM7 antibody [N2C2], Internal

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

List Price :

299 USD

Isotype :

IgG

Product Description :

Rabbit polyclonal antibody to MCM7 (minichromosome maintenance complex component 7)

CrossReactivity :

Background :

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human MCM7. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB, ChIP assay

Concentration :

0.76mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal (GTX110278) 7.5 % SDS-PAGE The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal (GTX110278) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Image Legend_5 :

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.