FLUOR DE LYS® deacetylase substrate | Enzo Life Sciences BML-KI104-0050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

FLUOR DE LYS® deacetylase substrate

catalog :

BML-KI104-0050

quantity :

50 µl

citations: 4

Reference
Decroos C, Christianson D. Design, Synthesis, and Evaluation of Polyamine Deacetylase Inhibitors, and High-Resolution Crystal Structures of Their Complexes with Acetylpolyamine Amidohydrolase. Biochemistry. 2015;54:4692-703 pubmed publisher
Singh R, Cho K, Padi S, Yu J, Haldar M, Mandal T, et al. Mechanism of N-Acylthiourea-mediated activation of human histone deacetylase 8 (HDAC8) at molecular and cellular levels. J Biol Chem. 2015;290:6607-19 pubmed publisher
Decroos C, Bowman C, Christianson D. Synthesis and evaluation of N?-acetylspermidine analogues as inhibitors of bacterial acetylpolyamine amidohydrolase. Bioorg Med Chem. 2013;21:4530-40 pubmed publisher
Jia H, Pallos J, Jacques V, Lau A, Tang B, Cooper A, et al. Histone deacetylase (HDAC) inhibitors targeting HDAC3 and HDAC1 ameliorate polyglutamine-elicited phenotypes in model systems of Huntington's disease. Neurobiol Dis. 2012;46:351-61 pubmed

product information

Product ID :

BML-KI104

Catalog Number :

BML-KI104-0050

Product Name :

FLUOR DE LYS® deacetylase substrate

Product Type :

Peptide

Size :

50 µl

list of Pubmed id :

26200446, 25605725, 23790721, 22590724

Product Description :

FLUOR DE LYS® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The FLUOR DE LYS® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the FLUOR DE LYS® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).

Purity :

≥95% (HPLC)

Buffer :

Supplied as a 50 mM solution (50 µl) in dimethylsulfoxide (DMSO).

Storage :

-80°C