cAMP complete ELISA kit | Enzo Life Sciences ADI-900-163 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

cAMP complete ELISA kit

catalog :

ADI-900-163

quantity :

96 wells

citations: 10

Reference
Liu H, Kuang X, Zhang Y, Ye Y, Li J, Liang L, et al. ADORA1 Inhibition Promotes Tumor Immune Evasion by Regulating the ATF3-PD-L1 Axis. Cancer Cell. 2020;37:324-339.e8 pubmed publisher
Banfi S, Gusarova V, Gromada J, Cohen J, Hobbs H. Increased thermogenesis by a noncanonical pathway in ANGPTL3/8-deficient mice. Proc Natl Acad Sci U S A. 2018;115:E1249-E1258 pubmed publisher
Malhotra N, Karthikeyan S, Chakraborti P. Phosphorylation of mycobacterial phosphodiesterase by eukaryotic-type Ser/Thr kinase controls its two distinct and mutually exclusive functionalities. J Biol Chem. 2017;292:17362-17374 pubmed publisher
Gobejishvili L, Ghare S, Khan R, Cambon A, Barker D, Barve S, et al. Misoprostol modulates cytokine expression through a cAMP pathway: Potential therapeutic implication for liver disease. Clin Immunol. 2015;161:291-9 pubmed publisher
Van Ginkel P, Yan M, Bhattacharya S, Polans A, Kenealey J. Natural products induce a G protein-mediated calcium pathway activating p53 in cancer cells. Toxicol Appl Pharmacol. 2015;288:453-62 pubmed publisher
Wang Y, McNutt M, Banfi S, Levin M, Holland W, Gusarova V, et al. Hepatic ANGPTL3 regulates adipose tissue energy homeostasis. Proc Natl Acad Sci U S A. 2015;112:11630-5 pubmed publisher
Park D, Shawl A, Ha T, Park K, Kim S, Kim U. Arginine Thiazolidine Carboxylate Stimulates Insulin Secretion through Production of Ca2+-Mobilizing Second Messengers NAADP and cADPR in Pancreatic Islets. PLoS ONE. 2015;10:e0134962 pubmed publisher
Kang S, Shin H, Kim S. Sinensetin enhances adipogenesis and lipolysis by increasing cyclic adenosine monophosphate levels in 3T3-L1 adipocytes. Biol Pharm Bull. 2015;38:552-8 pubmed publisher
de Jonge H, Tilly B, Hogema B, Pfau D, Kelley C, Kelley M, et al. cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland. Am J Physiol Cell Physiol. 2014;306:C343-53 pubmed publisher
Ríos Cardona D, Ricardo Gonzalez R, Chawla A, Ferrell J. A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes. Dev Biol. 2008;317:380-8 pubmed publisher

product information

Product ID :

ADI-900-163

Catalog Number :

ADI-900-163

Product Name :

cAMP complete ELISA kit

Product Type :

Kit

Size :

96 wells

list of Pubmed id :

26408955, 26341291, 26305978, 26247205, 25735898, 24259420, 18381211

Product Description :

This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.

SPECIES REACTIVITY :

Species independent

Background :

Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

ALTnames :

cyclic AMP

Uses :

For the quantitative determination of cAMP in cell lysates, culture supernatants, serum, saliva, and tissue from any species.

Application Summary :

ELISA, Colorimetric detection