GSK-3β ELISA kit | Enzo Life Sciences ADI-900-144 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

ELISA/assay

product name :

GSK-3β ELISA kit

catalog :

ADI-900-144

quantity :

96 wells

price :

587 USD

citations: 1

Published Application/Species/Dilution	Reference
	Ali M, El-Abhar H, Kamel M, Attia A. Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug. PLoS ONE. 2015;10:e0134648 pubmed publisher

product information

Product ID :

ADI-900-144

Catalog Number :

ADI-900-144

Product Name :

GSK-3β ELISA kit

Product Type :

Kit

Size :

96 wells

List Price (USD) :

587 USD

list of Pubmed id :

26262991

Product Description :

The GSK-3β EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours that measures pan GSK-3β. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

SPECIES REACTIVITY :

Human, Rat

Background :

Glycogen synthase kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3β is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3β. The phosphorylated serine binds to the GSK-3β priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3β is unable to phosphorylate β-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3β via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3β with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

ALTnames :

Glycogen synthase kinase-3β

Uses :

For the quantitative determination of GSK-3β in cell lysates of human and rat origin.

Application Summary :

ELISA, Colorimetric detection