This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
ELISA/assay
product name :
Direct cAMP ELISA kit
catalog :
ADI-900-066
quantity :
96 wells
citations: 34
Reference
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Zummo F, Krishnanda S, Georgiou M, O Harte F, Parthsarathy V, Cullen K, et al. Exendin-4 stimulates autophagy in pancreatic β-cells via the RAPGEF/EPAC-Ca2+-PPP3/calcineurin-TFEB axis. Autophagy. 2021;:1-17 pubmed publisher
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Truong M, Bilekova S, Choksi S, Li W, Bugaj L, Xu K, et al. Vertebrate cells differentially interpret ciliary and extraciliary cAMP. Cell. 2021;184:2911-2926.e18 pubmed publisher
Nakayama A, Albarrán Juárez J, Liang G, Roquid K, Iring A, Tonack S, et al. Disturbed flow-induced Gs-mediated signaling protects against endothelial inflammation and atherosclerosis. JCI Insight. 2020;5: pubmed publisher
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Hesse R, Lausser L, Gummert P, Schmid F, Wahler A, Schnack C, et al. Reduced cGMP levels in CSF of AD patients correlate with severity of dementia and current depression. Alzheimers Res Ther. 2017;9:17 pubmed publisher
Yoon Y, Kim S, Kim M, Yang H, Rhyu M, Park J. Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells. Molecules. 2015;20:22128-36 pubmed publisher
Kim K, Jung Yang H, Lee I, Kim K, Park J, Jeong H, et al. The aglycone of ginsenoside Rg3 enables glucagon-like peptide-1 secretion in enteroendocrine cells and alleviates hyperglycemia in type 2 diabetic mice. Sci Rep. 2015;5:18325 pubmed publisher
Rahimi H, Ghahremani M, Dehpour A, Sharifzadeh M, Ejtemaei Mehr S, Razmi A, et al. The Neuroprotective Effect of Lithium in cannabinoid Dependence is Mediated through Modulation of Cyclic AMP, ERK1/2 and GSK-3β Phosphorylation in Cerebellar Granular Neurons of Rat. Iran J Pharm Res. 2015;14:1123-35 pubmed
Xie K, Masuho I, Shih C, Cao Y, Sasaki K, Lai C, et al. Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling. elife. 2015;4: pubmed publisher
Molden B, Cooney K, West K, Van der Ploeg L, Baldini G. Temporal cAMP Signaling Selectivity by Natural and Synthetic MC4R Agonists. Mol Endocrinol. 2015;29:1619-33 pubmed publisher
Lundegaard P, Anastasaki C, Grant N, Sillito R, Zich J, Zeng Z, et al. MEK Inhibitors Reverse cAMP-Mediated Anxiety in Zebrafish. Chem Biol. 2015;22:1335-46 pubmed publisher
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product information
Product ID :
ADI-900-066
Catalog Number :
ADI-900-066
Product Name :
Direct cAMP ELISA kit
Product Type :
Kit
Size :
96 wells
list of Pubmed id :
26690397, 26675132, 26664379, 26613416, 26418335, 26388333, 26258013, 26189585, 25648146, 25472965
Product Description :
This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.
SPECIES REACTIVITY :
Species independent
Background :
Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
ALTnames :
cyclic AMP
Uses :
For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include tissue culture.
Application Summary :
ELISA, Colorimetric detection
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.