DNA-PK (Ab-2) Mouse mAb (18-2) | EMD Millipore NA57-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

DNA-PK (Ab-2) Mouse mAb (18-2)

catalog :

NA57-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher

product information

Catalog Number :

NA57-100UG

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

Anti-DNA-PK (Ab-2) Mouse mAb (18-2)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

5591 (Hu), 19090 (Ms), 360748 (Rt)

Host Name :

Clone :

18-2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-DNA-PK (Ab-2) Mouse mAb (18-2)

Cross Reactivity :

Human

Background :

DNA Dependent Protein Kinase, DNA-PK, is a nuclear serine/threonine protein kinase composed of two subunits, a ~470 kDa catalytic subunit, DNA-PKcs, and the Ku autoantigen, the heterodimeric DNA binding protein p70/p80. DNA-PK has been shown to phosphorylate a number of key proteins in vitro including p53, Sp1, myc, RPA, and RNA Pol I. DNA-PK activity requires association of DNA-PKcs with Ku, DNA binding, and the presence of DNA ends. Mutations in DNA-PKcs contribute to murine SCID phenotypes which leads to inefficient V(D)J recombination in developing T & B lymphocytes. Loss of DNA-PK activity in fibroblasts or lymphocytes also confers on cells hypersensitivity to killing by ionizing radiation. These two phenotypes implicate DNA-PK in the repair of DNA double strand breaks in all cells and V(D)J recombination in lymphocytes.

ALT Names :

DNA-DEPENDENT PROTEIN KINASE, DNA-PK, DNA-PKCS, DNAPDcs, DNPK1, HYRC, HYRC1, MGC189093, p220, p350, p460DNA-PK, Scid, slip, XRCC7, protein kinase, DNA-activated, catalytic polypeptide

Immunogen :

DNA-PK purified from HeLa cells

Specificity :

Recognizes the ~460 kDa DNA-PK protein.

Package Size :

100 ug

Uses :

IH, WB, IF, IP, NEUT, IH(P)

Storage :

For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.