This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
RNA polymerase II Antibody, clone CTD4H8
catalog :
05-623
quantity :
200 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CTD4H8

The same clone is also sold as:
reactivity :
Saccharum sp., Saccharomycetales, human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq
citations: 130
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 4g
Xiong Y, Li L, Zhang L, Cui Y, Wu C, Li H, et al. The bromodomain protein BRD4 positively regulates necroptosis via modulating MLKL expression. Cell Death Differ. 2019;: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1c
Sharma P, Lioutas A, Fernandez Fuentes N, Quilez J, Carbonell Caballero J, Wright R, et al. Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. Mol Cell. 2019;73:84-96.e7 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 6d
Fujimoto M, Takii R, Katiyar A, Srivastava P, Nakai A. Poly(ADP-Ribose) Polymerase 1 Promotes the Human Heat Shock Response by Facilitating Heat Shock Transcription Factor 1 Binding to DNA. Mol Cell Biol. 2018;38: pubmed publisher
  • immunoprecipitation; human; loading ...; fig 5f
  • western blot; human; 1:1000; loading ...; fig 5f
Slobodin B, Han R, Calderone V, Vrielink J, Loayza Puch F, Elkon R, et al. Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation. Cell. 2017;169:326-337.e12 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Audet Walsh E, Yee T, Tam I, Giguere V. Inverse Regulation of DHT Synthesis Enzymes 5?-Reductase Types 1 and 2 by the Androgen Receptor in Prostate Cancer. Endocrinology. 2017;158:1015-1021 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 5f
Ma J, Sanchez B, Hall D, Tremblay A, Di Marco S, Gallouzi I. STAT3 promotes IFNγ/TNFα-induced muscle wasting in an NF-κB-dependent and IL-6-independent manner. EMBO Mol Med. 2017;9:622-637 pubmed publisher
  • chromatin immunoprecipitation; rat; loading ...; tbl 1
Kumar A, Dumasia K, Deshpande S, Balasinor N. Direct regulation of genes involved in sperm release by estrogen and androgen through their receptors and coregulators. J Steroid Biochem Mol Biol. 2017;171:66-74 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 5a
  • chromatin immunoprecipitation; human; loading ...; fig 5b
Vispute S, Bu P, Le Y, Cheng X. Activation of GR but not PXR by dexamethasone attenuated acetaminophen hepatotoxicities via Fgf21 induction. Toxicology. 2017;378:95-106 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 5b
Hentati Kallel M, Le Jan S, Bernard P, Antonicelli F, Trussardi Regnier A. Histone deacetylases meet microRNA-associated MMP-9 expression regulation in glucocorticoid-sensitive and -resistant cell lines. Int J Oncol. 2017;50:717-726 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 5b
Vuono E, Mukherjee A, Vierra D, Adroved M, Hodson C, Deans A, et al. The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair. Sci Rep. 2016;6:36439 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 2e
Su C, Lin I, Tzeng T, Hsieh W, Hsu M. Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification. PLoS ONE. 2016;11:e0166090 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...
Tahmasebi S, Jafarnejad S, Tam I, Gonatopoulos Pournatzis T, Matta Camacho E, Tsukumo Y, et al. Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2. Proc Natl Acad Sci U S A. 2016;113:12360-12367 pubmed
  • chromatin immunoprecipitation; human; loading ...; fig 3j
Harrod A, Fulton J, Nguyen V, Periyasamy M, Ramos Garcia L, Lai C, et al. Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer. Oncogene. 2017;36:2286-2296 pubmed publisher
  • chromatin immunoprecipitation; human; fig 4
Wu H, Li S, Hu J, Yu X, Xu H, Chen Z, et al. Demystifying the mechanistic and functional aspects of p21 gene activation with double-stranded RNAs in human cancer cells. J Exp Clin Cancer Res. 2016;35:145 pubmed publisher
  • ChIP-Seq; human; fig 4
Gilan O, Lam E, Becher I, Lugo D, Cannizzaro E, Joberty G, et al. Functional interdependence of BRD4 and DOT1L in MLL leukemia. Nat Struct Mol Biol. 2016;23:673-81 pubmed publisher
  • western blot; human; 1:4000; fig 1
Kalantari R, Hicks J, Li L, Gagnon K, Sridhara V, Lemoff A, et al. Stable association of RNAi machinery is conserved between the cytoplasm and nucleus of human cells. RNA. 2016;22:1085-98 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 2d
Park S, Yi H, Suh N, Park Y, Koh J, Jeong S, et al. Inhibition of EHMT2/G9a epigenetically increases the transcription of Beclin-1 via an increase in ROS and activation of NF-?B. Oncotarget. 2016;7:39796-39808 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 7e
Yamashita D, Moriuchi T, Osumi T, Hirose F. Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α. J Biol Chem. 2016;291:11619-34 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 6g
Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher
  • western blot; human; 1:1000; fig 7
Scheckel C, Drapeau E, Frias M, Park C, Fak J, Zucker Scharff I, et al. Regulatory consequences of neuronal ELAV-like protein binding to coding and non-coding RNAs in human brain. elife. 2016;5: pubmed publisher
  • chromatin immunoprecipitation; human; 1:100; fig 5b
Wang X, Jung Y, Jun S, Lee S, Wang W, Schneider A, et al. PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness. Nat Commun. 2016;7:10633 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 5
Ancelin K, Syx L, Borensztein M, Ranisavljevic N, Vassilev I, Briseño Roa L, et al. Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation. elife. 2016;5: pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 4c
Hessmann E, Zhang J, Chen N, Hasselluhn M, Liou G, Storz P, et al. NFATc4 Regulates Sox9 Gene Expression in Acinar Cell Plasticity and Pancreatic Cancer Initiation. Stem Cells Int. 2016;2016:5272498 pubmed publisher
  • chromatin immunoprecipitation; human
Madsen A, Bozickovic O, Bjune J, Mellgren G, Sagen J. Metformin inhibits hepatocellular glucose, lipid and cholesterol biosynthetic pathways by transcriptionally suppressing steroid receptor coactivator 2 (SRC-2). Sci Rep. 2015;5:16430 pubmed publisher
  • chromatin immunoprecipitation; human; 10 ul/mg; fig 8
Su S, Zhao Q, He C, Huang D, Liu J, Chen F, et al. miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program. Nat Commun. 2015;6:8523 pubmed publisher
  • immunohistochemistry; Saccharum sp.; 1:300
Moraes I, Yuan Z, Liu S, Souza G, Garcia B, Casas Mollano J. Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane. PLoS ONE. 2015;10:e0134586 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 6
Tan K, Fujimoto M, Takii R, Takaki E, Hayashida N, Nakai A. Mitochondrial SSBP1 protects cells from proteotoxic stresses by potentiating stress-induced HSF1 transcriptional activity. Nat Commun. 2015;6:6580 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 6
Gillespie M, Gold E, Ramsey S, Podolsky I, Aderem A, Ranish J. An LXR-NCOA5 gene regulatory complex directs inflammatory crosstalk-dependent repression of macrophage cholesterol efflux. EMBO J. 2015;34:1244-58 pubmed publisher
  • western blot; human; fig 4
García Carmona Y, Cols M, Ting A, Radigan L, Yuk F, Zhang L, et al. Differential induction of plasma cells by isoforms of human TACI. Blood. 2015;125:1749-58 pubmed publisher
  • western blot; Saccharomycetales
Allepuz Fuster P, Martínez Fernández V, Garrido Godino A, Alonso Aguado S, Hanes S, Navarro F, et al. Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation. Nucleic Acids Res. 2014;42:13674-88 pubmed
  • chromatin immunoprecipitation; human; fig 1
Maruyama A, Mimura J, Itoh K. Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding. Nucleic Acids Res. 2014;42:13599-614 pubmed publisher
  • immunoprecipitation; human
Zhou Z, Wang R, Yang X, Lu X, Zhang Q, Wang Y, et al. The cAMP-responsive element binding protein (CREB) transcription factor regulates furin expression during human trophoblast syncytialization. Placenta. 2014;35:907-18 pubmed publisher
  • chromatin immunoprecipitation; human
Wright R, Slemmons K, Vaughan A. Estradiol induces gene proximity and MLL-MLLT3 fusion in an activation-induced cytidine deaminase-mediated pathway. Leuk Lymphoma. 2015;56:1460-5 pubmed publisher
  • immunoprecipitation; Saccharomycetales
Mirón García M, Garrido Godino A, Martínez Fernández V, Fernández Pévida A, Cuevas Bermúdez A, Martín Expósito M, et al. The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription. Nucleic Acids Res. 2014;42:9666-76 pubmed publisher
  • chromatin immunoprecipitation; rat
Baby N, Li Y, Ling E, Lu J, Dheen S. Runx1t1 (Runt-related transcription factor 1; translocated to, 1) epigenetically regulates the proliferation and nitric oxide production of microglia. PLoS ONE. 2014;9:e89326 pubmed publisher
  • immunoprecipitation; mouse
Meng L, Person R, Huang W, Zhu P, Costa Mattioli M, Beaudet A. Truncation of Ube3a-ATS unsilences paternal Ube3a and ameliorates behavioral defects in the Angelman syndrome mouse model. PLoS Genet. 2013;9:e1004039 pubmed publisher
  • chromatin immunoprecipitation; human
Maruyama A, Mimura J, Harada N, Itoh K. Nrf2 activation is associated with Z-DNA formation in the human HO-1 promoter. Nucleic Acids Res. 2013;41:5223-34 pubmed publisher
  • chromatin immunoprecipitation; mouse
Panigrahi S, Vasileva A, Wolgemuth D. Sp1 transcription factor and GATA1 cis-acting elements modulate testis-specific expression of mouse cyclin A1. PLoS ONE. 2012;7:e47862 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 3m
Modzelewski A, Holmes R, Hilz S, Grimson A, Cohen P. AGO4 regulates entry into meiosis and influences silencing of sex chromosomes in the male mouse germline. Dev Cell. 2012;23:251-64 pubmed publisher
  • immunocytochemistry; human
Sims R, Rojas L, Beck D, Bonasio R, Schüller R, Drury W, et al. The C-terminal domain of RNA polymerase II is modified by site-specific methylation. Science. 2011;332:99-103 pubmed publisher
  • chromatin immunoprecipitation; human
Karius T, Schnekenburger M, Ghelfi J, Walter J, Dicato M, Diederich M. Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines. Biochem Pharmacol. 2011;81:1329-42 pubmed publisher
  • chromatin immunoprecipitation; human
Milagre I, Nunes M, Moutinho M, Rivera I, Fuso A, Scarpa S, et al. Chromatin-modifying agents increase transcription of CYP46A1, a key player in brain cholesterol elimination. J Alzheimers Dis. 2010;22:1209-21 pubmed publisher
  • immunoprecipitation; human
Guo D, Prins R, Dang J, Kuga D, Iwanami A, Soto H, et al. EGFR signaling through an Akt-SREBP-1-dependent, rapamycin-resistant pathway sensitizes glioblastomas to antilipogenic therapy. Sci Signal. 2009;2:ra82 pubmed publisher
  • immunocytochemistry; human; 1:500
Nayak A, Glöckner Pagel J, Vaeth M, Schumann J, Buttmann M, Bopp T, et al. Sumoylation of the transcription factor NFATc1 leads to its subnuclear relocalization and interleukin-2 repression by histone deacetylase. J Biol Chem. 2009;284:10935-46 pubmed publisher
  • chromatin immunoprecipitation; mouse
Schnekenburger M, Talaska G, Puga A. Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation. Mol Cell Biol. 2007;27:7089-101 pubmed
Chappidi N, Nascakova Z, Boleslavska B, Zellweger R, Isik E, Andrs M, et al. Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops. Mol Cell. 2019;: pubmed publisher
Baquero Perez B, Antanaviciute A, Yonchev I, Carr I, Wilson S, Whitehouse A. The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus. elife. 2019;8: pubmed publisher
Rossi M, Bucci G, Rizzotto D, Bordo D, Marzi M, Puppo M, et al. LncRNA EPR controls epithelial proliferation by coordinating Cdkn1a transcription and mRNA decay response to TGF-β. Nat Commun. 2019;10:1969 pubmed publisher
Kumar M, Kasti K, Balakrishnan L, Milavetz B. Directed Nucleosome Sliding during the Formation of the Simian Virus 40 Particle Exposes DNA Sequences Required for Early Transcription. J Virol. 2019;93: pubmed publisher
Bornelöv S, Reynolds N, Xenophontos M, Gharbi S, Johnstone E, Floyd R, et al. The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression. Mol Cell. 2018;71:56-72.e4 pubmed publisher
Cannizzaro E, Bannister A, Han N, Alendar A, Kouzarides T. DDX3X RNA helicase affects breast cancer cell cycle progression by regulating expression of KLF4. FEBS Lett. 2018;592:2308-2322 pubmed publisher
Kim M, Xia B, Suh H, Lee S, Jun S, Lien E, et al. PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis. Dev Cell. 2018;44:582-596.e4 pubmed publisher
Tan X, Banerjee P, Liu X, Yu J, Gibbons D, Wu P, et al. The epithelial-to-mesenchymal transition activator ZEB1 initiates a prometastatic competing endogenous RNA network. J Clin Invest. 2018;128:1267-1282 pubmed publisher
Yan T, Ooi W, Qamra A, Cheung A, Ma D, Sundaram G, et al. HoxC5 and miR-615-3p target newly evolved genomic regions to repress hTERT and inhibit tumorigenesis. Nat Commun. 2018;9:100 pubmed publisher
Chen J, Teo B, Cai Y, Wee S, Lu J. The linker histone H1.2 is a novel component of the nucleolar organizer regions. J Biol Chem. 2018;293:2358-2369 pubmed publisher
Bugge M, Bergstrom B, Eide O, Solli H, Kjønstad I, Stenvik J, et al. Surface Toll-like receptor 3 expression in metastatic intestinal epithelial cells induces inflammatory cytokine production and promotes invasiveness. J Biol Chem. 2017;292:15408-15425 pubmed publisher
Lu W, Katzenellenbogen B. Estrogen Receptor-? Modulation of the ER?-p53 Loop Regulating Gene Expression, Proliferation, and Apoptosis in Breast Cancer. Horm Cancer. 2017;8:230-242 pubmed publisher
Ono R, Masuya M, Ishii S, Katayama N, Nosaka T. Eya2, a Target Activated by Plzf, Is Critical for PLZF-RARA-Induced Leukemogenesis. Mol Cell Biol. 2017;37: pubmed publisher
Ganti K, Mukherji A, Surjit M, Li M, Chambon P. Similarities and differences in the transcriptional control of expression of the mouse TSLP gene in skin epidermis and intestinal epithelium. Proc Natl Acad Sci U S A. 2017;114:E951-E960 pubmed publisher
Yang C, Li Z, Bhatt T, Dickler M, Giri D, Scaltriti M, et al. Acquired CDK6 amplification promotes breast cancer resistance to CDK4/6 inhibitors and loss of ER signaling and dependence. Oncogene. 2017;36:2255-2264 pubmed publisher
Martínez Alonso M, Hengrung N, Fodor E. RNA-Free and Ribonucleoprotein-Associated Influenza Virus Polymerases Directly Bind the Serine-5-Phosphorylated Carboxyl-Terminal Domain of Host RNA Polymerase II. J Virol. 2016;90:6014-6021 pubmed publisher
Takemata N, Oda A, Yamada T, Galipon J, Miyoshi T, Suzuki Y, et al. Local potentiation of stress-responsive genes by upstream noncoding transcription. Nucleic Acids Res. 2016;44:5174-89 pubmed publisher
Banerjee A, Kim Y, Kim T. A novel virus-inducible enhancer of the interferon-β gene with tightly linked promoter and enhancer activities. Nucleic Acids Res. 2014;42:12537-54 pubmed publisher
Hsu C, Liu J, Wu P, Guan H, Chen Y, Lin A, et al. Identification of a new androgen receptor (AR) co-regulator BUD31 and related peptides to suppress wild-type and mutated AR-mediated prostate cancer growth via peptide screening and X-ray structure analysis. Mol Oncol. 2014;8:1575-87 pubmed publisher
He Y, Tsuei J, Wan Y. Biological functional annotation of retinoic acid alpha and beta in mouse liver based on genome-wide binding. Am J Physiol Gastrointest Liver Physiol. 2014;307:G205-18 pubmed publisher
Khurana J, Wang X, Chen X, Perlman D, Landweber L. Transcription-independent functions of an RNA polymerase II subunit, Rpb2, during genome rearrangement in the ciliate, Oxytricha trifallax. Genetics. 2014;197:839-49 pubmed publisher
Sonzogni S, Ogara M, Belluscio L, Castillo D, Scassa M, Cánepa E. p19INK4d is involved in the cellular senescence mechanism contributing to heterochromatin formation. Biochim Biophys Acta. 2014;1840:2171-83 pubmed publisher
Shimoyama S, Kasai S, Kahn Perlès B, Kikuchi H. Dephosphorylation of Sp1 at Ser-59 by protein phosphatase 2A (PP2A) is required for induction of CYP1A1 transcription after treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin or omeprazole. Biochim Biophys Acta. 2014;1839:107-15 pubmed publisher
Parikh N, Hilsenbeck S, Creighton C, Dayaram T, Shuck R, Shinbrot E, et al. Effects of TP53 mutational status on gene expression patterns across 10 human cancer types. J Pathol. 2014;232:522-33 pubmed publisher
Angulo Rojo C, Manning Cela R, Aguirre A, Ortega A, Lopez Bayghen E. Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA. ASN Neuro. 2013;5:e00130 pubmed publisher
Tanaka Nakanishi A, Yasunaga J, Takai K, Matsuoka M. HTLV-1 bZIP factor suppresses apoptosis by attenuating the function of FoxO3a and altering its localization. Cancer Res. 2014;74:188-200 pubmed publisher
Huang V, Zheng J, Qi Z, Wang J, Place R, Yu J, et al. Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells. PLoS Genet. 2013;9:e1003821 pubmed publisher
Seidel C, Schnekenburger M, Dicato M, Diederich M. Antiproliferative and proapoptotic activities of 4-hydroxybenzoic acid-based inhibitors of histone deacetylases. Cancer Lett. 2014;343:134-46 pubmed publisher
Matsui M, Chu Y, Zhang H, Gagnon K, Shaikh S, Kuchimanchi S, et al. Promoter RNA links transcriptional regulation of inflammatory pathway genes. Nucleic Acids Res. 2013;41:10086-109 pubmed publisher
Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher
Garrido Godino A, García López M, Navarro F. Correct assembly of RNA polymerase II depends on the foot domain and is required for multiple steps of transcription in Saccharomyces cerevisiae. Mol Cell Biol. 2013;33:3611-26 pubmed publisher
Chen W, Jia W, Wang K, Si X, Zhu S, Duan T, et al. Distinct roles for CBP and p300 on the RA-mediated expression of the meiosis commitment gene Stra8 in mouse embryonic stem cells. PLoS ONE. 2013;8:e66076 pubmed publisher
Li X, Xu Y, Chen Y, Chen S, Jia X, Sun T, et al. SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/?-catenin signal network. Cancer Lett. 2013;336:379-89 pubmed publisher
Udofa E, Stringer B, Gade P, Mahony D, Buzza M, Kalvakolanu D, et al. The transcription factor C/EBP-? mediates constitutive and LPS-inducible transcription of murine SerpinB2. PLoS ONE. 2013;8:e57855 pubmed publisher
Wong J, Mehta V, Voronova A, Coutu J, Ryan T, Shelton M, et al. ?-catenin is essential for efficient in vitro premyogenic mesoderm formation but can be partially compensated by retinoic acid signalling. PLoS ONE. 2013;8:e57501 pubmed publisher
Tashiro S, Asano T, Kanoh J, Ishikawa F. Transcription-induced chromatin association of RNA surveillance factors mediates facultative heterochromatin formation in fission yeast. Genes Cells. 2013;18:327-39 pubmed publisher
He S, Khan D, Winter S, Seiser C, Davie J. Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin. J Cell Physiol. 2013;228:1525-35 pubmed publisher
Zhou J, Joplin D, Cross J, Templeton D. Sulforaphane inhibits prostaglandin E2 synthesis by suppressing microsomal prostaglandin E synthase 1. PLoS ONE. 2012;7:e49744 pubmed publisher
Zonta E, Bittencourt D, Samaan S, Germann S, Dutertre M, Auboeuf D. The RNA helicase DDX5/p68 is a key factor promoting c-fos expression at different levels from transcription to mRNA export. Nucleic Acids Res. 2013;41:554-64 pubmed publisher
Wier E, Neighoff J, Sun X, Fu K, Wan F. Identification of an N-terminal truncation of the NF-?B p65 subunit that specifically modulates ribosomal protein S3-dependent NF-?B gene expression. J Biol Chem. 2012;287:43019-29 pubmed publisher
Matsui M, Prakash T, Corey D. Transcriptional silencing by single-stranded RNAs targeting a noncoding RNA that overlaps a gene promoter. ACS Chem Biol. 2013;8:122-6 pubmed publisher
Hu J, Chen Z, Xia D, Wu J, Xu H, Ye Z. Promoter-associated small double-stranded RNA interacts with heterogeneous nuclear ribonucleoprotein A2/B1 to induce transcriptional activation. Biochem J. 2012;447:407-16 pubmed publisher
Pinheiro A, Nunes M, Milagre I, Rodrigues E, Silva M, de Almeida I, et al. Demethylation of the coding region triggers the activation of the human testis-specific PDHA2 gene in somatic tissues. PLoS ONE. 2012;7:e38076 pubmed publisher
Chujo M, Tarumoto Y, Miyatake K, Nishida E, Ishikawa F. HIRA, a conserved histone chaperone, plays an essential role in low-dose stress response via transcriptional stimulation in fission yeast. J Biol Chem. 2012;287:23440-50 pubmed publisher
Cherian M, Wilson E, Shapiro D. A competitive inhibitor that reduces recruitment of androgen receptor to androgen-responsive genes. J Biol Chem. 2012;287:23368-80 pubmed publisher
Bhatla T, Wang J, Morrison D, Raetz E, Burke M, Brown P, et al. Epigenetic reprogramming reverses the relapse-specific gene expression signature and restores chemosensitivity in childhood B-lymphoblastic leukemia. Blood. 2012;119:5201-10 pubmed publisher
Afelik S, Qu X, Hasrouni E, Bukys M, Deering T, Nieuwoudt S, et al. Notch-mediated patterning and cell fate allocation of pancreatic progenitor cells. Development. 2012;139:1744-53 pubmed publisher
Jani D, Lutz S, Hurt E, Laskey R, Stewart M, Wickramasinghe V. Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export. Nucleic Acids Res. 2012;40:4562-73 pubmed publisher
Shin Y, Kim J. The role of EZH2 in the regulation of the activity of matrix metalloproteinases in prostate cancer cells. PLoS ONE. 2012;7:e30393 pubmed publisher
Dey S, Xue Y, Joachimiak M, Friedland G, Burnett J, Zhou Q, et al. Mutual information analysis reveals coevolving residues in Tat that compensate for two distinct functions in HIV-1 gene expression. J Biol Chem. 2012;287:7945-55 pubmed publisher
Ianculescu I, Wu D, Siegmund K, Stallcup M. Selective roles for cAMP response element-binding protein binding protein and p300 protein as coregulators for androgen-regulated gene expression in advanced prostate cancer cells. J Biol Chem. 2012;287:4000-13 pubmed publisher
Lee Y, Lee J, Ho C, Hong Q, Yu S, Tzeng C, et al. miRNA-34b as a tumor suppressor in estrogen-dependent growth of breast cancer cells. Breast Cancer Res. 2011;13:R116 pubmed publisher
Grbic D, Degagné E, Larrivée J, Bilodeau M, Vinette V, Arguin G, et al. P2Y6 receptor contributes to neutrophil recruitment to inflamed intestinal mucosa by increasing CXC chemokine ligand 8 expression in an AP-1-dependent manner in epithelial cells. Inflamm Bowel Dis. 2012;18:1456-69 pubmed publisher
Purcell D, Jeong K, Bittencourt D, Gerke D, Stallcup M. A distinct mechanism for coactivator versus corepressor function by histone methyltransferase G9a in transcriptional regulation. J Biol Chem. 2011;286:41963-71 pubmed publisher
Garcia Lopez M, Pelechano V, Mirón García M, Garrido Godino A, Garcia A, Calvo O, et al. The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation. Genetics. 2011;189:1235-48 pubmed publisher
Gorski J, Savage K, Mulligan J, McDade S, Blayney J, Ge Z, et al. Profiling of the BRCA1 transcriptome through microarray and ChIP-chip analysis. Nucleic Acids Res. 2011;39:9536-48 pubmed publisher
Gizard F, Zhao Y, Findeisen H, Qing H, Cohn D, Heywood E, et al. Transcriptional regulation of S phase kinase-associated protein 2 by NR4A orphan nuclear receptor NOR1 in vascular smooth muscle cells. J Biol Chem. 2011;286:35485-93 pubmed publisher
Son J, Chae C, Hwang J, Park Z, Im S. Enhanced chromatin accessibility and recruitment of JUNB mediate the sustained IL-4 expression in NFAT1 deficient T helper 2 cells. PLoS ONE. 2011;6:e22042 pubmed publisher
Zhou D, Pan Y. Gestational low protein diet selectively induces the amino acid response pathway target genes in the liver of offspring rats through transcription factor binding and histone modifications. Biochim Biophys Acta. 2011;1809:549-56 pubmed publisher
Chuang Y, Huang W, Park S, Persaud S, Hung C, Ho P, et al. Promyelocytic leukemia protein in retinoic acid-induced chromatin remodeling of Oct4 gene promoter. Stem Cells. 2011;29:660-9 pubmed publisher
Furumai R, Ito A, Ogawa K, Maeda S, Saito A, Nishino N, et al. Histone deacetylase inhibitors block nuclear factor-?B-dependent transcription by interfering with RNA polymerase II recruitment. Cancer Sci. 2011;102:1081-7 pubmed publisher
Singh S, Wilczynska K, Grzybowski A, Yester J, Osrah B, Bryan L, et al. The unique transcriptional activation domain of nuclear factor-I-X3 is critical to specifically induce marker gene expression in astrocytes. J Biol Chem. 2011;286:7315-26 pubmed publisher
Chakravarthy H, Ormsbee B, Mallanna S, Rizzino A. Rapid activation of the bivalent gene Sox21 requires displacement of multiple layers of gene-silencing machinery. FASEB J. 2011;25:206-18 pubmed publisher
Lee M, Partridge N. Parathyroid hormone activation of matrix metalloproteinase-13 transcription requires the histone acetyltransferase activity of p300 and PCAF and p300-dependent acetylation of PCAF. J Biol Chem. 2010;285:38014-22 pubmed publisher
Garcia A, Rosonina E, Manley J, Calvo O. Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation. Mol Cell Biol. 2010;30:5180-93 pubmed publisher
Huang Y, Chen H, Teng S. Intragenic transcription of a noncoding RNA modulates expression of ASP3 in budding yeast. RNA. 2010;16:2085-93 pubmed publisher
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product information
Catalog Number :
05-623
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-RNA polymerase II Antibody, clone CTD4H8
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P24928
Host Name :
Mouse
Antigen :
RNA polymerase II
Clone :
CTD4H8
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-RNA polymerase II Antibody, clone CTD4H8
Cross Reactivity :
Human;Rat;Mouse;Yeast ( S. cerevisiae )
Background :
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Immunogen :
Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Specificity :
This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
Package Size :
200 µg
Uses :
Western Blotting;ChIP-seq
Storage :
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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