Casein Kinase 2α Antibody, 1AD9 | EMD Millipore 05-1431 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Casein Kinase 2α Antibody, 1AD9

catalog :

05-1431

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1AD9

The same clone is also sold as:

Enzo Life Sciences: BML-SA163-0100
Santa Cruz Biotechnology: sc-12738

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human	Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami Hamada K, Nakagawa R, et al. N-terminal phosphorylation of HP1Î± increases its nucleosome-binding specificity. Nucleic Acids Res. 2014;42:12498-511 pubmed publisher
western blot; human	Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. PLoS ONE. 2013;8:e54382 pubmed publisher
	Schael S, Nüchel J, Muller S, Petermann P, Kormann J, Pérez Otaño I, et al. Casein kinase 2 phosphorylation of protein kinase C and casein kinase 2 substrate in neurons (PACSIN) 1 protein regulates neuronal spine formation. J Biol Chem. 2013;288:9303-12 pubmed publisher
	Kroonen J, Artesi M, Capraro V, Nguyen Khac M, Willems M, Chakravarti A, et al. Casein kinase 2 inhibition modulates the DNA damage response but fails to radiosensitize malignant glioma cells. Int J Oncol. 2012;41:776-82 pubmed publisher

product information

Catalog Number :

05-1431

Subcategory :

Cell Signaling

Product Name :

Anti-Casein Kinase 2α Antibody, 1AD9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68400

Host Name :

Mouse

Antigen :

Casein Kinase 2α

Clone :

1AD9

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Casein Kinase 2α Antibody, 1AD9

Cross Reactivity :

Human;Mouse;Rat

Background :

Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.

ALT Names :

CK2 catalytic subunit alpha;CKII alpha;Casein kinase II subunit alpha;Casein kinase 2, alpha 1 polypeptide;Casein kinase II alpha 1 subunit;Casein kinase II-alpha subunit;Protein kinase CK2

Immunogen :

Purified recombinant α-subunit of casein kinase 2.

Specificity :

The antibody binds specifically to the catalytic α-subunit of casein kinase2 (CK-2α). The antibody immunoprecipitates a protein with a molecular weight of 44 kD, CK-2α subunit. A 26 kD protein band, the β-subunit of the heterotetrameric holoenzyme α2β2, is coprecipitated with the CK-2α subunit. In western blots with extracts of kidney tissue, the antibody detects various protein bands between 40 and 44 kDa. Using 1 μg/mL of antibody, the lower detection limit for the holoenzyme is 500 ng in Western blot and 100 ng in dot blot; the detection limits for the α-subunit are 300 ng and 60 ng, respectively.

Package Size :

100 μg

Uses :

Immunocytochemistry;Western Blotting;Immunohistochemistry (Paraffin);Immunoprecipitation

Storage :

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.