RNA polymerase II subunit B1 Antibody, clone 4F8 | EMD Millipore 04-1569 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RNA polymerase II subunit B1 Antibody, clone 4F8

catalog :

04-1569

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

4F8

The same clone is also sold as:

Active Motif: 61081

reactivity :

human, mouse

application :

western blot, ELISA, chromatin immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000; loading ...; fig 4a	Mayfield J, Irani S, Escobar E, Zhang Z, Burkholder N, Robinson M, et al. Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb. elife. 2019;8: pubmed publisher

product information

Catalog Number :

04-1569

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Rat

Antigen :

RNA polymerase II subunit B1

Clone :

4F8

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

Cross Reactivity :

Mouse;Human

Background :

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.

ALT Names :

DNA-directed RNA polymerase II A;DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA polymerase II subunit B1;RNA-directed RNA polymerase II subunit RPB1;polymerase (RNA) II (DNA directed) polypeptide A (220kD);polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

Immunogen :

Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.

Specificity :

This antibody recognizes RNA polymerase II subunit B1.

Package Size :

100 μg

Uses :

Western Blotting;ELISA;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt