Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal | EMD Millipore 04-1100 product information

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal

catalog :

04-1100

quantity :

100 µL

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

EP565Y

reactivity :

human, mouse, rabbit

application :

western blot, immunocytochemistry

more info or order :

EMD Millipore product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; rabbit; 1:1000; fig 5	Zhang J, Sun D, Fu Q, Cao Q, Zhang H, Zhang K. Bone mesenchymal stem cells differentiate into myofibroblasts in the tumor microenvironment. Oncol Lett. 2016;12:644-650 pubmed

product information

Catalog Number :

04-1100

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q15797

Host Name :

Rabbit

Antigen :

Smad1

Clone :

EP565Y

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal

Cross Reactivity :

Human;Mouse

Background :

Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

ALT Names :

MAD, mothers against decapentaplegic homolog 1;MAD, mothers against decapentaplegic homolog 1 (Drosophila);Mad-related protein 1;Mothers against DPP homolog 1;SMAD 1;SMAD family member 1 ;SMAD, mothers against DPP homolog 1 ;SMAD, mothers against DPP homolog 1 (Drosophila);Sma- and Mad-related protein 1 ;TGF-beta signaling protein 1;Transforming growth factor-beta-signaling protein 1 ;transforming growth factor-beta signaling protein 1

Immunogen :

Synthetic peptide from human Smad1.

Specificity :

This antibody recognizes Smad1.

Package Size :

100 µL

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

more info or order :

EMD Millipore product webpage