Anti-HIF-1α Antibody, clone EP1215Y, rabbit monoclonal | EMD Millipore 04-1006 product information

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Anti-HIF-1α Antibody, clone EP1215Y, rabbit monoclonal

catalog :

04-1006

quantity :

100 µL

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

EP1215Y

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

EMD Millipore product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1a	Alig S, Stampnik Y, Pircher J, Rotter R, Gaitzsch E, Ribeiro A, et al. The tyrosine phosphatase SHP-1 regulates hypoxia inducible factor-1Î± (HIF-1Î±) protein levels in endothelial cells under hypoxia. PLoS ONE. 2015;10:e0121113 pubmed publisher
immunocytochemistry; rat; 1:1000	Ramamoorthy P, Shi H. Ischemia induces different levels of hypoxia inducible factor-1? protein expression in interneurons and pyramidal neurons. Acta Neuropathol Commun. 2014;2:51 pubmed publisher
	Hoppe G, Lee T, Yoon S, Yu M, Peachey N, Rayborn M, et al. Inducing a visceral organ to protect a peripheral capillary bed: stabilizing hepatic HIF-1? prevents oxygen-induced retinopathy. Am J Pathol. 2014;184:1890-9 pubmed publisher
	Goetzenich A, Hatam N, Preuss S, Moza A, Bleilevens C, Roehl A, et al. The role of hypoxia-inducible factor-1? and vascular endothelial growth factor in late-phase preconditioning with xenon, isoflurane and levosimendan in rat cardiomyocytes. Interact Cardiovasc Thorac Surg. 2014;18:321-8 pubmed publisher
	Zhang Z, Yan J, Taheri S, Liu K, Shi H. Hypoxia-inducible factor 1 contributes to N-acetylcysteine's protection in stroke. Free Radic Biol Med. 2014;68:8-21 pubmed publisher
	Yan J, Zhou B, Taheri S, Shi H. Differential effects of HIF-1 inhibition by YC-1 on the overall outcome and blood-brain barrier damage in a rat model of ischemic stroke. PLoS ONE. 2011;6:e27798 pubmed publisher

product information

Catalog Number :

04-1006

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-HIF-1α Antibody, clone EP1215Y, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16665

Host Name :

Rabbit

Antigen :

HIF-1α

Clone :

EP1215Y

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-HIF-1α Antibody, clone EP1215Y, rabbit monoclonal

Cross Reactivity :

Human;Rat

Background :

Hypoxia, a condition of low tissue O2 concentration, plays an important role in normal physiological processes and tumor formation. Under hypoxic conditions mammalian cells up regulate the expression of hypoxic genes, including induction of angiogenesis and a switch to anaerobic metabolism, in order to survive. HIF-1 (Hypoxia Inducible Factor-1) is one of the key regulators of the transcriptional response to oxygen deprivation (1). HIF-1 is composed of two subunits, HIF-1αand HIF-1β also known as aryl hydrocarbon receptor nuclear translocator (ARNT)) that are members of the basic helix-loop-helix (bHLH) Per-Arnt-Sim (PAS) (bHLH-PAS) family of transcription factors. HIF-1 is essential for angiogenesis, embryonic development, and is associated with tumor progression, erythropoiesis, vascular development/remodeling, vasodilation, and glucose/energy metabolism. The over expression of HIF-1αhas been demonstrated in many common human cancers including prostate and breast, in which HIF-1α levels are associated with increase vascularitry and tumor progression. Besides physiological hypoxia, genetic abnormalities frequently detected in human cancers, such as loss of function mutations (Von Hippel-Lindau, p53, and PTEN), are associated with induction of HIF1 activity and expression of HIF-1-inducible genes (1).

ALT Names :

ARNT interacting protein ;ARNT-interacting protein ;Basic-helix-loop-helix-PAS protein MOP1 ;HIF-1 alpha ;HIF1 alpha ;Member of PAS protein 1 ;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) ;hypoxia-inducible factor 1, alpha subunit ;hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) ;member of PAS superfamily 1

Immunogen :

Synthetic peptide corresponding to residues near the C-terminus of human HIF-1α.

Specificity :

This antibody recognizes HIF-1α at and around the C-terminus.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

more info or order :

EMD Millipore product webpage