LEAF™ Purified anti-mouse IFN-γ | BioLegend 505707 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse IFN-γ

catalog :

505707

quantity :

1 mg

price :

460 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

R4-6A2

reactivity :

mouse

application :

immunohistochemistry, neutralization

citations: 13

Published Application/Species/Sample/Dilution	Reference
ELISA; mouse; fig 5	Thornton T, Delgado P, Chen L, Salas B, Krementsov D, Fernández M, et al. Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nat Commun. 2016;7:10553 pubmed publisher
	Mastroianni J, Stickel N, Andrlova H, Hanke K, Melchinger W, Duquesne S, et al. miR-146a Controls Immune Response in the Melanoma Microenvironment. Cancer Res. 2019;79:183-195 pubmed publisher
	Deason K, Troutman T, Jain A, Challa D, Mandraju R, Brewer T, et al. BCAP links IL-1R to the PI3K-mTOR pathway and regulates pathogenic Th17 cell differentiation. J Exp Med. 2018;215:2413-2428 pubmed publisher
	Vaartjes D, Nandakumar K, Holmdahl R, Raposo B. Increased salt exposure affects both lymphoid and myeloid effector functions, influencing innate-associated disease but not T-cell-associated autoimmunity. Immunology. 2018;: pubmed publisher
	Liang D, Tian L, You R, Halpert M, Konduri V, Baig Y, et al. AIMp1 Potentiates TH1 Polarization and Is Critical for Effective Antitumor and Antiviral Immunity. Front Immunol. 2017;8:1801 pubmed publisher
	Cardoso V, Chesne J, Ribeiro H, García Cassani B, Carvalho T, Bouchery T, et al. Neuronal regulation of type 2 innate lymphoid cells via neuromedin U. Nature. 2017;549:277-281 pubmed publisher
	Clua P, Kanmani P, Zelaya H, Tada A, Kober A, Salva S, et al. Peptidoglycan from Immunobiotic Lactobacillus rhamnosus Improves Resistance of Infant Mice to Respiratory Syncytial Viral Infection and Secondary Pneumococcal Pneumonia. Front Immunol. 2017;8:948 pubmed publisher
	Strandt H, Pinheiro D, Kaplan D, Wirth D, GRATZ I, Hammerl P, et al. Neoantigen Expression in Steady-State Langerhans Cells Induces CTL Tolerance. J Immunol. 2017;199:1626-1634 pubmed publisher
	Barsheshet Y, Wildbaum G, Levy E, Vitenshtein A, Akinseye C, Griggs J, et al. CCR8+FOXp3+ Treg cells as master drivers of immune regulation. Proc Natl Acad Sci U S A. 2017;114:6086-6091 pubmed publisher
	Cho S, Roh K, Park J, Park Y, Lee M, Cho S, et al. Hydrolysis of Hyaluronic Acid in Lymphedematous Tissue Alleviates Fibrogenesis via TH1 Cell-Mediated Cytokine Expression. Sci Rep. 2017;7:35 pubmed publisher
	Angela M, Endo Y, Asou H, Yamamoto T, Tumes D, Tokuyama H, et al. Fatty acid metabolic reprogramming via mTOR-mediated inductions of PPAR? directs early activation of T cells. Nat Commun. 2016;7:13683 pubmed publisher
	Chou T, Chuang Y, Hsieh W, Chang P, Liu H, Mo S, et al. Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1? for Th17 suppression. Nat Commun. 2016;7:11904 pubmed publisher
	Lombes A, Durand A, Charvet C, RiviÃ¨re M, Bonilla N, Auffray C, et al. Adaptive Immune-like Î³/Î´ T Lymphocytes Share Many Common Features with Their Î±/Î² T Cell Counterparts. J Immunol. 2015;195:1449-58 pubmed publisher

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ELISA Capture, ELISPOT Capture, IHC, Neut

Cat # :

505707

Clone :

R4-6A2

Item :

LEAF™ Purified anti-mouse IFN-γ

Isotype :

Rat IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

1 mg

Price (USD) :

460 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

Partially-purified, native mouse IFN-γ

Application Notes :

ELISA Capture 1-4,6,10,11 or ELISPOT Capture 5 : The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 µg/mL. ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 505812) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. Neutralization 1,2,9 : The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713). Additional reported applications (for the relevant formats) include: immunohistochemical staining 3,6,8 of acetone-fixed frozen tissue sections. Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 and 430804) are specially developed and recommended.