LEAF™ Purified anti-mouse CD152 | BioLegend 106308 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse CD152

catalog :

106308

quantity :

500 μg

price :

250 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

UC10-4B9

reactivity :

mouse

application :

neutralization, immunoprecipitation, flow cytometry

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 3e	Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Shi B, Geng J, Wang Y, Wei H, Walters B, Li W, et al. Foxp1 Negatively Regulates T Follicular Helper Cell Differentiation and Germinal Center Responses by Controlling Cell Migration and CTLA-4. J Immunol. 2018;200:586-594 pubmed publisher
	McDonald Hyman C, Muller J, Loschi M, Thangavelu G, Saha A, Kumari S, et al. The vimentin intermediate filament network restrains regulatory T cell suppression of graft-versus-host disease. J Clin Invest. 2018;128:4604-4621 pubmed publisher
	Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
	Crosby E, Wei J, Yang X, Lei G, Wang T, Liu C, et al. Complimentary mechanisms of dual checkpoint blockade expand unique T-cell repertoires and activate adaptive anti-tumor immunity in triple-negative breast tumors. Oncoimmunology. 2018;7:e1421891 pubmed publisher
	Klann J, Kim S, Remedios K, He Z, Metz P, Lopez J, et al. Integrin Activation Controls Regulatory T Cell-Mediated Peripheral Tolerance. J Immunol. 2018;200:4012-4023 pubmed publisher
	Tung S, Boardman D, Sen M, Letizia M, Peng Q, Cianci N, et al. Regulatory T cell-derived extracellular vesicles modify dendritic cell function. Sci Rep. 2018;8:6065 pubmed publisher
	Marangoni F, Zhang R, Mani V, Thelen M, Ali Akbar N, Warner R, et al. Tumor Tolerance-Promoting Function of Regulatory T Cells Is Optimized by CD28, but Strictly Dependent on Calcineurin. J Immunol. 2018;200:3647-3661 pubmed publisher
	Wallrapp A, Riesenfeld S, Burkett P, Abdulnour R, Nyman J, Dionne D, et al. The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation. Nature. 2017;549:351-356 pubmed publisher
	Goel S, Decristo M, Watt A, BrinJones H, Sceneay J, Li B, et al. CDK4/6 inhibition triggers anti-tumour immunity. Nature. 2017;548:471-475 pubmed publisher
	Nawaf M, Ulvmar M, Withers D, McConnell F, Gaspal F, Webb G, et al. Concurrent OX40 and CD30 Ligand Blockade Abrogates the CD4-Driven Autoimmunity Associated with CTLA4 and PD1 Blockade while Preserving Excellent Anti-CD8 Tumor Immunity. J Immunol. 2017;199:974-981 pubmed publisher
	Klocke K, Holmdahl R, Wing K. CTLA-4 expressed by FOXP3+ regulatory T cells prevents inflammatory tissue attack and not T-cell priming in arthritis. Immunology. 2017;152:125-137 pubmed publisher
	Naskar D, Teng F, Felix K, Bradley C, WU H. Synthetic Retinoid AM80 Ameliorates Lung and Arthritic Autoimmune Responses by Inhibiting T Follicular Helper and Th17 Cell Responses. J Immunol. 2017;198:1855-1864 pubmed publisher
	Sugita S, Horie S, Yamada Y, Mochizuki M. Inhibition of B-cell activation by retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2010;51:5783-8 pubmed publisher

product information

Antigen :

CD152

Apps. Abbrev. :

Flow Cytometry, IP, ELISA, Block

Cat # :

106308

Clone :

UC10-4B9

Item :

LEAF™ Purified anti-mouse CD152

Isotype :

Armenian Hamster IgG

Other Names :

Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56

Size :

500 μg

Price (USD) :

250 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

LEAF

Immunogen :

Mouse CTLA-4-mouse IgG2a fusion protein

Application Notes :

The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro stimulation, in vitro and in vivo blocking 1-4 of ligand binding, and as ELISA capture antibody 5 . To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 106327).