product summary
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company name :
Agrisera
product type :
antibody
product name :
His-tag 6xHis
catalog :
AS11 1771
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IgG2b, clone HIS.H8 / EH158
application :
western blot, ELISA, immunoprecipitation, dot blot
more info or order :
product information
Product number :
AS11 1771
Product name :
His-tag 6xHis
Immunogen :
KLH-conjugated synthetic peptide 6xHis
Host :
Mouse
Clonality :
Monoclonal
Clone number :
IgG2b, clone HIS.H8 / EH158
Purity :
Protein A purified
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunlocalization (IL), Western blot (WB)
Related products :
collection of antibodies to various tagsPlant and algal protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000 (WB)
Confirmed reactivity :
6xHis
Predicted reactivity :
6xHis
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Antibody is present in 10 mM PBS, pH 7.2
Selected references :
Häggmark-Månberg et al. (2016). Autoantibody targets in vaccine-associated narcolepsy. Autoimmunity. 2016 Sep;49(6):421-433. Epub 2016 May 20.
Pubmed ID's :
27206786
Background :
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For storage at 4°C for several days to weeks, sodium azide to final 0.1 % can be added. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Working dilution for ELISA, IL and IP needs to be determined experimentally.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.