This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1his p66shc (corrected)
catalog :
32574
citations: 3
Reference
Purba E, Saita E, Akhouri R, xd6 fverstedt L, Wilken G, Skoglund U, et al. Allosteric activation of preformed EGF receptor dimers by a single ligand binding event. Front Endocrinol (Lausanne). 2022;13:1042787 pubmed publisher
Huang H, Svoboda R, Lazenby A, Saowapa J, Chaika N, Ding K, et al. Up-regulation of N-cadherin by Collagen I-activated Discoidin Domain Receptor 1 in Pancreatic Cancer Requires the Adaptor Molecule Shc1. J Biol Chem. 2016;291:23208-23223 pubmed
Nemoto S, Finkel T. Redox regulation of forkhead proteins through a p66shc-dependent signaling pathway. Science. 2002;295:2450-2 pubmed
product information
Catalog Number :
32574
Product Name :
pcDNA3.1his p66shc (corrected)
article :
doi10.1126/science.1069004
id437
pubmed_id11884717
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 His
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid is a corrected version of pcDNA3.1his p66shc (Addgene plasmid 10972 http://www.addgene.org/10972). D2N and S60P mutations present in the original sample have been corrected in this plasmid.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSHC, SHCA
geneSHC1
id6464
genbank_ids
mutation
namep66shc
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
146
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA