pCDNA3-myc-his-BACH1 P47A | Addgene 17644 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA3-myc-his-BACH1 P47A

catalog :

17644

citations: 1

Reference
Cantor S, Drapkin R, Zhang F, Lin Y, Han J, Pamidi S, et al. The BRCA1-associated protein BACH1 is a DNA helicase targeted by clinically relevant inactivating mutations. Proc Natl Acad Sci U S A. 2004;101:2357-62 pubmed

product information

Catalog Number :

17644

Product Name :

pCDNA3-myc-his-BACH1 P47A

article :

doi	10.1073/pnas.0308717101
id	2002
pubmed_id	14983014

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1(+)/myc-His A

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5493

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

origin :

pi :

alt_names

BRIP1

FANCJ

cloning

clone_method	Restriction Enzyme
cloning_site_3	Apa1
cloning_site_5	Not1
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BACH1, FANCJ, OF
gene	BRIP1
id	83990

genbank_ids

AF360549

mutation

To generate the tumor-associated mutant, the proline at position 47 was changed to an alanine by Quikchange site-directed mutagenesis (Stratagene) using the following primers: [CATTGTTTGTTGGAGAGTGCCACAGGAAGTGGAAAAGC] and [GCTTTTTCCACTTCCTGTGGCACTCTCCAACAAACAATG]

name

BACH1

shRNA_sequence

size

species

9606

Homo sapiens