This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GLI K12
catalog :
16419
citations: 6
Reference
Vlčková K, Ondrušová L, Vachtenheim J, Réda J, Dundr P, Zadinová M, et al. Survivin, a novel target of the Hedgehog/GLI signaling pathway in human tumor cells. Cell Death Dis. 2016;7:e2048 pubmed publisher
Iglesias Bartolomé R, Torres D, Marone R, Feng X, Martin D, Simaan M, et al. Inactivation of a Gα(s)-PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis. Nat Cell Biol. 2015;17:793-803 pubmed publisher
Zahreddine H, Culjkovic Kraljacic B, Assouline S, Gendron P, Romeo A, Morris S, et al. The sonic hedgehog factor GLI1 imparts drug resistance through inducible glucuronidation. Nature. 2014;511:90-3 pubmed publisher
Amable L, Gavin E, Kudo K, Meng E, Rocconi R, Shevde L, et al. GLI1 upregulates C-JUN through a specific 130-kDa isoform. Int J Oncol. 2014;44:655-61 pubmed publisher
Kasaai B, Gaumond M, Moffatt P. Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation. J Biol Chem. 2013;288:13278-94 pubmed publisher
Kinzler K, Ruppert J, Bigner S, Vogelstein B. The GLI gene is a member of the Kruppel family of zinc finger proteins. Nature. 1988;332:371-4 pubmed
product information
Catalog Number :
16419
Product Name :
GLI K12
article :
doi10.1038/332371a0
id1817
pubmed_id2832761
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript KS
backbone_mutation
backbone_originStratagene
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).
origin :
37
pi :
alt_names
Gli-1
glioma-associated oncogene homolog 1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3T3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGLI, PAPA8, PPD1
geneGLI1
id2735
genbank_ids
mutation
nameGLI
shRNA_sequence
size3601
species
9606
Homo sapiens
tags
resistance markers :
553
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA