pLenti-lox-GFP shRNA p19-2 | Addgene 14091 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti-lox-GFP shRNA p19-2

catalog :

14091

citations: 2

Reference
Papp S, Huber A, Jordan S, Kriebs A, Nguyen M, Moresco J, et al. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. elife. 2015;4: pubmed publisher
Sage J, Miller A, Perez Mancera P, Wysocki J, Jacks T. Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Nature. 2003;424:223-8 pubmed

product information

Catalog Number :

14091

Product Name :

pLenti-lox-GFP shRNA p19-2

article :

doi	10.1038/nature01764
id	1229
pubmed_id	12853964

bacterial resistance :

Ampicillin

cloning :

backbone

pLL3.7

backbone_mutation

backbone_origin

Available from Addgene (#11795)

backbone_size

8000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

Cre/Lox

growth notes :

p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.

origin :

pi :

alt_names

p19ARF

ARF-INK4a

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BglII
promoter
sequencing_primer_3
sequencing_primer_5	H1
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19, p19ARF
gene	Cdkn2a
id	12578

genbank_ids

mutation

name

p19 ARF shRNA -2

shRNA_sequence

size

species

10090

Mus musculus