This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSIRIP-puro shRNA p19-1
catalog :
14089
citations: 1
Reference
Sage J, Miller A, Perez Mancera P, Wysocki J, Jacks T. Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Nature. 2003;424:223-8 pubmed
product information
Catalog Number :
14089
Product Name :
pSIRIP-puro shRNA p19-1
article :
doi10.1038/nature01764
id1229
pubmed_id12853964
bacterial resistance :
Ampicillin
cloning :
backbonepSIRIP-puro
backbone_mutation
backbone_originTodd Golub Lab
backbone_size6500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
p19 ARF shRNA expressed from a self-inactivating retroviral vector. See author's map for information about the pSIRIP-puro vector backbone.
origin :
37
pi :
alt_names
p19ARF
ARF-INK4a
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BglII
promoter
sequencing_primer_3
sequencing_primer_5H1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19, p19ARF
geneCdkn2a
id12578
genbank_ids
mutation
namep19 ARF shRNA - 1
shRNA_sequence
size50
species
10090
Mus musculus
tags
resistance markers :
225
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA