pcDNA3.1his p66shc | Addgene 10972 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.1his p66shc

catalog :

10972

citations: 2

Reference
Ranieri S, Pani G. Monitoring nutrient signaling through the longevity protein p66(SHC¹). Methods Mol Biol. 2013;965:341-53 pubmed publisher
Nemoto S, Finkel T. Redox regulation of forkhead proteins through a p66shc-dependent signaling pathway. Science. 2002;295:2450-2 pubmed

product information

Catalog Number :

10972

Product Name :

pcDNA3.1his p66shc

article :

doi	10.1126/science.1069004
id	437
pubmed_id	11884717

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1 His

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note that this plasmid contains D2N and S60P mutations in the p66/SHC sequence when compared to GenBank entries NP_892113.4 and NP_001123512.1. This is the original plasmid as used in the associated publication listed below. For a corrected pcDNA3.1his p66shc, please see Addgene plasmid 32574 http://www.addgene.org/32574

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	BGHrev
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	SHC, SHCA
gene	SHC1
id	6464

genbank_ids

mutation

name

p66shc

shRNA_sequence

size

species

9606

Homo sapiens