pCMV p16 INK4A | Addgene 10916 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV p16 INK4A

catalog :

10916

citations: 5

Reference
Abaandou L, Sharma A, Shiloach J. Knockout of the caspase 8-associated protein 2 gene improves recombinant protein expression in HEK293 cells through up-regulation of the cyclin-dependent kinase inhibitor 2A gene. Biotechnol Bioeng. 2021;118:186-198 pubmed publisher
Che H, Li J, Li Y, Ma C, Liu H, Qin J, et al. p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle. elife. 2020;9: pubmed publisher
Bao X, Hu X. Up-regulated expression of E2F2 is necessary for p16INK4a-induced cartilage injury. BMC Musculoskelet Disord. 2018;19:334 pubmed publisher
Niu L, Cheng C, Li M, Yang S, Hu B, Chong C, et al. ID1-induced p16/IL6 axis activation contributes to the resistant of hepatocellular carcinoma cells to sorafenib. Cell Death Dis. 2018;9:852 pubmed publisher
Medema R, Herrera R, Lam F, Weinberg R. Growth suppression by p16ink4 requires functional retinoblastoma protein. Proc Natl Acad Sci U S A. 1995;92:6289-93 pubmed

product information

Catalog Number :

10916

Product Name :

pCMV p16 INK4A

article :

doi	10.1073/pnas.92.14.6289
id	423
pubmed_id	7603984

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5446

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4. The insert corresponds to isoform 1 but is missing the first 8 amino acids.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16
gene	CDKN2A
id	1029

genbank_ids

mutation

name

p16 INK4

shRNA_sequence

size

472

species

9606

Homo sapiens