This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Epitomics, Ascent Scientific, MitoSciences
product type :
antibody
product name :
Vinculin antibody [SPM227]
catalog :
ab18058
quantity :
500 ul
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SPM227
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 52
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 5
Johnson S, Dempsey C, Parker C, Mironov A, Bradley H, Saha V. Acute lymphoblastic leukaemia cells produce large extracellular vesicles containing organelles and an active cytoskeleton. J Extracell Vesicles. 2017;6:1294339 pubmed publisher
  • immunohistochemistry; human; loading ...; fig s1a
Han X, Li P, Yang Z, Huang X, Wei G, Sun Y, et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun. 2017;8:14639 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4a
  • western blot; mouse; 1:1000; loading ...; fig 8a
Ziosi M, Di Meo I, Kleiner G, Gao X, Barca E, Sanchez Quintero M, et al. Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway. EMBO Mol Med. 2017;9:96-111 pubmed publisher
  • western blot; human; loading ...; fig 6a
Guillén Boixet J, Buzon V, Salvatella X, Mendez R. CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. elife. 2016;5: pubmed publisher
  • western blot; human; 1:3000; fig 3a
Sun H, Ding C, Zhang H, Gao J. Let?7 miRNAs sensitize breast cancer stem cells to radiation?induced repression through inhibition of the cyclin D1/Akt1/Wnt1 signaling pathway. Mol Med Rep. 2016;14:3285-92 pubmed publisher
  • western blot; human; loading ...; fig 5b
Hirano A, Shi G, Jones C, Lipzen A, Pennacchio L, Xu Y, et al. A Cryptochrome 2 mutation yields advanced sleep phase in humans. elife. 2016;5: pubmed publisher
  • immunocytochemistry; human; fig 9
Zhang X, Adderley S, Breslin J. Activation of RhoA, but Not Rac1, Mediates Early Stages of S1P-Induced Endothelial Barrier Enhancement. PLoS ONE. 2016;11:e0155490 pubmed publisher
  • western blot; human; fig 2
Hall A, Lu W, Godfrey J, Antonov A, Paicu C, Moxon S, et al. The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration. Cell Death Dis. 2016;7:e2184 pubmed publisher
  • western blot; human; 1:3000; fig s4
Voigt F, Wiedemann L, Zuliani C, Querques I, Sebe A, Mátés L, et al. Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering. Nat Commun. 2016;7:11126 pubmed publisher
  • western blot; human; fig 4
Sears C, Cooney S, Chin Sinex H, Mendonca M, Turchi J. DNA damage response (DDR) pathway engagement in cisplatin radiosensitization of non-small cell lung cancer. DNA Repair (Amst). 2016;40:35-46 pubmed publisher
  • western blot; human; fig 3
Gelot C, Guirouilh Barbat J, Le Guen T, Dardillac E, Chailleux C, Canitrot Y, et al. The Cohesin Complex Prevents the End Joining of Distant DNA Double-Strand Ends. Mol Cell. 2016;61:15-26 pubmed publisher
  • western blot; human; fig 1
Renaud E, Barascu A, Rosselli F. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res. 2016;44:648-56 pubmed publisher
  • western blot; human; fig 2
Kennedy A, Vallurupalli M, Chen L, Crompton B, Cowley G, Vazquez F, et al. Functional, chemical genomic, and super-enhancer screening identify sensitivity to cyclin D1/CDK4 pathway inhibition in Ewing sarcoma. Oncotarget. 2015;6:30178-93 pubmed publisher
  • immunocytochemistry; human; fig 1
Hwang J, Byun M, Kim A, Kim K, Cho H, Lee Y, et al. Extracellular Matrix Stiffness Regulates Osteogenic Differentiation through MAPK Activation. PLoS ONE. 2015;10:e0135519 pubmed publisher
  • western blot; mouse; 1:10,000; fig s6a
Lovisa S, LeBleu V, Tampe B, Sugimoto H, Vadnagara K, Carstens J, et al. Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis. Nat Med. 2015;21:998-1009 pubmed publisher
  • western blot; human; 1:1000; fig s5
Luna Sánchez M, Díaz Casado E, Barca E, Tejada M, Montilla García Ã, Cobos E, et al. The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene. EMBO Mol Med. 2015;7:670-87 pubmed publisher
  • western blot; human
Shi D, Murty V, Gu W. PCDH10, a novel p53 transcriptional target in regulating cell migration. Cell Cycle. 2015;14:857-66 pubmed publisher
  • western blot; mouse
Waters C, Saldivar J, Amin Z, Schrock M, Huebner K. FHIT loss-induced DNA damage creates optimal APOBEC substrates: Insights into APOBEC-mediated mutagenesis. Oncotarget. 2015;6:3409-19 pubmed
  • immunocytochemistry; human
Holle A, Tang X, Vijayraghavan D, Vincent L, Fuhrmann A, Choi Y, et al. In situ mechanotransduction via vinculin regulates stem cell differentiation. Stem Cells. 2013;31:2467-77 pubmed publisher
Bogliolo M, Bluteau D, Lespinasse J, Pujol R, Vasquez N, d Enghien C, et al. Biallelic truncating FANCM mutations cause early-onset cancer but not Fanconi anemia. Genet Med. 2018;20:458-463 pubmed publisher
Gao Y, Wang Z, Hao Q, Li W, Xu Y, Zhang J, et al. Loss of ER? induces amoeboid-like migration of breast cancer cells by downregulating vinculin. Nat Commun. 2017;8:14483 pubmed publisher
Dominguez Brauer C, Hao Z, Elia A, Fortin J, Nechanitzky R, Brauer P, et al. Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation. Cell Stem Cell. 2016;19:205-216 pubmed publisher
Muter J, Brighton P, Lucas E, Lacey L, Shmygol A, Quenby S, et al. Progesterone-Dependent Induction of Phospholipase C-Related Catalytically Inactive Protein 1 (PRIP-1) in Decidualizing Human Endometrial Stromal Cells. Endocrinology. 2016;157:2883-93 pubmed publisher
Pfaltzgraff E, Roth G, Miller P, Gintzig A, Ohi R, Bader D. Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities. Mol Biol Cell. 2016;27:1990-9 pubmed publisher
Kang Y, Roife D, Lee Y, Lv H, Suzuki R, Ling J, et al. Transforming Growth Factor-? Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors. Clin Cancer Res. 2016;22:4934-4946 pubmed
Rebbani K, Marchio A, Ezzikouri S, Afifi R, Kandil M, Bahri O, et al. TP53 R72P polymorphism modulates DNA methylation in hepatocellular carcinoma. Mol Cancer. 2015;14:74 pubmed publisher
Ding Y, Yang M, Yang Z, Luo R, Lu X, Huang N, et al. Cooperative control of blood compatibility and re-endothelialization by immobilized heparin and substrate topography. Acta Biomater. 2015;15:150-63 pubmed publisher
Matsui T, Arima Y, Takemoto N, Iwata H. Cell patterning on polylactic acid through surface-tethered oligonucleotides. Acta Biomater. 2015;13:32-41 pubmed publisher
Kanada M, Zhang J, Yan L, Sakurai T, Terakawa S. Endothelial cell-initiated extravasation of cancer cells visualized in zebrafish. Peerj. 2014;2:e688 pubmed publisher
Chandran J, Kazanis I, Clapcote S, Ogawa F, Millar J, Porteous D, et al. Disc1 variation leads to specific alterations in adult neurogenesis. PLoS ONE. 2014;9:e108088 pubmed publisher
Gehrke I, Bouchard E, Beiggi S, Poeppl A, Johnston J, Gibson S, et al. On-target effect of FK866, a nicotinamide phosphoribosyl transferase inhibitor, by apoptosis-mediated death in chronic lymphocytic leukemia cells. Clin Cancer Res. 2014;20:4861-72 pubmed publisher
Ding Y, Yang Z, Bi C, Yang M, Xu S, Lu X, et al. Directing vascular cell selectivity and hemocompatibility on patterned platforms featuring variable topographic geometry and size. ACS Appl Mater Interfaces. 2014;6:12062-70 pubmed publisher
Grabarz A, Guirouilh Barbat J, Barascu A, Pennarun G, Genet D, Rass E, et al. A role for BLM in double-strand break repair pathway choice: prevention of CtIP/Mre11-mediated alternative nonhomologous end-joining. Cell Rep. 2013;5:21-8 pubmed publisher
Carnevale J, Ross L, Puissant A, Banerji V, Stone R, DeAngelo D, et al. SYK regulates mTOR signaling in AML. Leukemia. 2013;27:2118-28 pubmed publisher
Renaud E, Rosselli F. FANC pathway promotes UV-induced stalled replication forks recovery by acting both upstream and downstream Pol? and Rev1. PLoS ONE. 2013;8:e53693 pubmed publisher
Coley W, Rayavarapu S, Pandey G, Sabina R, van der Meulen J, Ampong B, et al. The molecular basis of skeletal muscle weakness in a mouse model of inflammatory myopathy. Arthritis Rheum. 2012;64:3750-9 pubmed publisher
Feig J, Vengrenyuk Y, Reiser V, Wu C, Statnikov A, Aliferis C, et al. Regression of atherosclerosis is characterized by broad changes in the plaque macrophage transcriptome. PLoS ONE. 2012;7:e39790 pubmed publisher
Banerji V, Frumm S, Ross K, Li L, Schinzel A, Hahn C, et al. The intersection of genetic and chemical genomic screens identifies GSK-3? as a target in human acute myeloid leukemia. J Clin Invest. 2012;122:935-47 pubmed publisher
Ladhani O, Sánchez Martinez C, Orgaz J, Jimenez B, Volpert O. Pigment epithelium-derived factor blocks tumor extravasation by suppressing amoeboid morphology and mesenchymal proteolysis. Neoplasia. 2011;13:633-42 pubmed
Sabra G, Vermette P. Endothelial cell responses towards low-fouling surfaces bearing RGD in a three-dimensional environment. Exp Cell Res. 2011;317:1994-2006 pubmed publisher
Castillo P, Bogliolo M, Surralles J. Coordinated action of the Fanconi anemia and ataxia telangiectasia pathways in response to oxidative damage. DNA Repair (Amst). 2011;10:518-25 pubmed publisher
Cherubini G, Naim V, Caruso P, Burla R, Bogliolo M, Cundari E, et al. The FANC pathway is activated by adenovirus infection and promotes viral replication-dependent recombination. Nucleic Acids Res. 2011;39:5459-73 pubmed publisher
Soenen S, De Meyer S, Dresselaers T, Vande Velde G, Pareyn I, Braeckmans K, et al. MRI assessment of blood outgrowth endothelial cell homing using cationic magnetoliposomes. Biomaterials. 2011;32:4140-50 pubmed publisher
Holtz M, Misra R. Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium. BMC Dev Biol. 2011;11:18 pubmed publisher
Ceccaldi R, Briot D, Larghero J, Vasquez N, Dubois d Enghien C, Chamousset D, et al. Spontaneous abrogation of the G?DNA damage checkpoint has clinical benefits but promotes leukemogenesis in Fanconi anemia patients. J Clin Invest. 2011;121:184-94 pubmed publisher
Soenen S, Himmelreich U, Nuytten N, De Cuyper M. Cytotoxic effects of iron oxide nanoparticles and implications for safety in cell labelling. Biomaterials. 2011;32:195-205 pubmed publisher
Rimmelé P, Komatsu J, Hupe P, Roulin C, Barillot E, Dutreix M, et al. Spi-1/PU.1 oncogene accelerates DNA replication fork elongation and promotes genetic instability in the absence of DNA breakage. Cancer Res. 2010;70:6757-66 pubmed publisher
Kucharzewska P, Welch J, Svensson K, Belting M. Ornithine decarboxylase and extracellular polyamines regulate microvascular sprouting and actin cytoskeleton dynamics in endothelial cells. Exp Cell Res. 2010;316:2683-91 pubmed publisher
Pasini D, Malatesta M, Jung H, Walfridsson J, Willer A, Olsson L, et al. Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes. Nucleic Acids Res. 2010;38:4958-69 pubmed publisher
Soenen S, Nuytten N, De Meyer S, De Smedt S, De Cuyper M. High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling. Small. 2010;6:832-42 pubmed publisher
Hahn C, Berchuck J, Ross K, Kakoza R, Clauser K, Schinzel A, et al. Proteomic and genetic approaches identify Syk as an AML target. Cancer Cell. 2009;16:281-94 pubmed publisher
Raya A, Rodriguez Piza I, Guenechea G, Vassena R, Navarro S, Barrero M, et al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature. 2009;460:53-9 pubmed publisher
product information
Catalog Number :
ab18058
Product Name :
Vinculin antibody [SPM227]
Host Species :
Mouse
Clonality :
monoclonal
Product Type :
Primary antibodies
Size :
500 ul
SwissProtID :
P18206
Isotype :
IgG1
CrossReactivity :
Reacts with: Human, Mouse, Rat, Hamster.
Application Summary :
Flow Cyt, ICC/IF, IHC-P, IP, WB
company information
Abcam
330 Cambridge Science Park
Cambridge
CB4 0FL UK
orders@abcam.com
44 (0)1223 696000

1 Kendall Square, Ste 341
Cambridge, MA 02139-1517
USA
us.orders@abcam.com
617 225 2272, 888-77-ABCAM (22226)

www.abcam.com
888-7722226
headquarters: UK