14-3-3 epsilon antibody (clone 5A5) | ATGen AYW0817 product information

product summary

request information :

company name :

ATGen

product type :

antibody

product name :

14-3-3 epsilon antibody (clone 5A5)

catalog :

AYW0817

quantity :

100 ul / 50 ul

price :

290 / 170 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

ATGen product webpage

product information

Catalog Number :

AYW0817

Product Name :

14-3-3 epsilon antibody (clone 5A5)

Product Type :

Primary Antibody

List Price :

290 / 170 USD

Clonality :

Monoclonal

Host Name :

mouse

Clone :

5A5

Conjugate :

unconjugated

Isotype :

IgG2a, k

Product Description :

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms,beta, gamma,epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

CrossReactivity :

Human

SpeciesSummary :

Human

Background :

unconjugated

Immunogen :

Recombinant human 14-3-3 epsilon (1-255aa) purified from E. coli

Purity :

By protein-G affinity chromatography

Packaging :

Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.

PackageSize :

100 ul / 50 ul

Concentration :

1mg/ml

Uses :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000.

Application Summary :

ELISA, WB (Cell lysate)

Storage :

Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

General References :

kino T, Pavlakis GN. (2004) DNA Cell Biol. 23(4):193-205 Jin DY, et al., (1996) Nature. 382(6589):308

more info or order :

ATGen product webpage