overview

Labome lists 566997 antibody products against 58324 genes, including 18816 human, 14158 mouse, and 12188 rat genes.

This is a summary of WB antibody products in Labome database. There are 169291 monoclonal WB antibody products, 191770 polyclonal WB antibody products, 6131 recombinant WB antibody products, including WB antibodies against 18066 human genes.

If you are looking for WB antibodies against a specific gene, type in gene name in the box above and search. In the resultant gene webpage, select 'antibody' link for the correct gene to go to the antibody webpage, and use the WB filter there to obtain a list of WB antibodies for that gene.

about Western Blotting

Western blot (alternatively, immunoblot) is a proteomic technique used to detect specific proteins in cell or tissue lysates or extracts.

Immunoblotting can be used to determine the presence of specific protein, and its size/molecular weight (if the lysates or extracts are separated under denaturing condition). It can also be used to determine the relative abundance of a specific protein, although the absolute quantity can not be determined. In addition, if phospho antibody is used as the primary antibody, the phosphorylation status of a specific protein can be determined, usually after the protein has been immunoprecipitated.

A typical Western blot protocol is provided here for reference. Variations to this protocol exist, and can be found throughout the web. One important thing is that for each Western blot, it is alway a good idea or perhaps imperative to have both negative controls and positive controls. One of the most common controls (to ensure consistent sample loading) is to use beta-actin antibody blot after the Western bloting of primary antibodies and stripping. see commonly used beta-actin antibody.

Clonality

Labome product database lists 169291 monoclonal WB antibodies, 191770 polyclonal WB antibodies, 6131 recombinant WB antibodies.

Gene Species

WB antibodies correspond to 18066 human genes, 13954 mouse genes, 12035 rat genes, 2534 bovine genes, 2515 dogs genes, 1186 zebrafish genes, 868 chicken genes, 579 thale cress genes, 485 pigs genes, 322 fruit fly genes, 277 domestic rabbit genes, 227 Chinese hamsters genes, 220 chimpanzee genes, 174 roundworm genes, 170 Xenopus laevis genes, 163 rhesus macaque genes, 140 domestic horse genes, 138 domestic sheep genes, 127 Saccharomyces cerevisiae S288C genes, 101 cat genes, 93 guinea pig genes, 57 Chlamydomonas reinhardtii genes, 38 crab eating macaque genes, 32 domestic goat genes, 31 orang utan genes, 29 western clawed frog genes, 16 fission yeast genes, 14 gorilla genes, 13 Borreliella burgdorferi B31 genes, 10 Human gammaherpesvirus 8 genes, 8 Syrian golden hamster genes, 8 Mycobacterium tuberculosis H37Rv genes, 7 Human immunodeficiency virus 1 genes, 7 SARS-CoV-2 genes, 6 Epstein Barr virus genes, 5 soybeans genes, 5 Atlantic salmon genes, 5 Gairdner's shrew-mouse genes, 5 Pseudomonas aeruginosa PAO1 genes, 5 Influenza A virus (A/Puerto Rico/8/1934(H1N1)) genes, 5 Bordetella pertussis Tohama I genes, 5 SARS coronavirus genes, 4 baboon genes, 4 Herpes simplex virus type 1 genes, 4 Human cytomegalovirus genes, 4 Plasmodium falciparum 3D7 genes, 4 two-rowed barley genes, 4 Thermosynechococcus elongatus BP-1 genes, 3 tomato genes, 3 potatoes genes, 3 silk moth genes, 3 rainbow trout genes, 3 Japanese rice fish genes, 3 common marmoset genes, 3 black ferret genes, 3 Hepatitis B virus genes, 3 green monkey genes, 3 Chlamydia trachomatis D/UW-3/CX genes, 3 Aspergillus fumigatus Af293 genes, 3 Dictyostelium discoideum AX4 genes, 3 Escherichia coli O157:H7 str. Sakai genes.

Host Species

In Labome product database, among WB antibodies, 186652 from domestic rabbit host, 156518 from mouse host, 14411 from domestic goat host, 3937 from rat host, 2184 from domestic sheep host, 1623 from human host, 702 from chicken host, 323 from guinea-pigs host, 248 from hamsters host, 177 from unidentified host, 158 from llama host, 98 from E. coli host, 32 from alpaca host, 24 from Syrian golden hamster host, 18 from African ass host.

Antigen Modification

In Labome product database, among WB antibodies, 3062 products are against phosphorylated antigens, 31 products are against acetylated antigens, 3 products are against methylated antigens.

Antibody Conjugation

In Labome product database, among WB antibodies, 3881 are conjugated with biotin, 1510 are conjugated with HRP, 662 are conjugated with FITC, 408 are conjugated with PE, 320 are conjugated with APC, 317 are conjugated with AP, 177 are conjugated with PerCP, 120 are conjugated with ATTO 594, 114 are conjugated with DyLight488, 104 are conjugated with RPE, 92 are conjugated with AF488, 91 are conjugated with BMP, 87 are conjugated with ATTO 488, 71 are conjugated with AF647, 65 are conjugated with DyLight 550, 63 are conjugated with DyLight 650, 59 are conjugated with Pacific Blue, 53 are conjugated with DyLight549, 50 are conjugated with DyLight649, 47 are conjugated with DyLight680, 41 are conjugated with DyLight800, 41 are conjugated with AF700, 36 are conjugated with ATTO 655, 34 are conjugated with Atto 700, 19 are conjugated with DyLight 405, 18 are conjugated with DyLight 594, 13 are conjugated with pacific orange, 12 are conjugated with Alexa Fluor 555, 12 are conjugated with Cy3, 12 are conjugated with texas red, 12 are conjugated with Alexa Fluor 594, 9 are conjugated with Alexa Fluor 568, 9 are conjugated with TRITC, 8 are conjugated with efluor 660, 8 are conjugated with rhodamine, 8 are conjugated with Alexa Fluor 405, 7 are conjugated with DyLight 350, 7 are conjugated with APC/Cy7, 5 are conjugated with Alexa Fluor 680, 4 are conjugated with Alexa Fluor 532, 4 are conjugated with PerCP/Cy5.5, 3 are conjugated with ATTO 647N, 3 are conjugated with agarose, 2 are conjugated with Cy5.