This is a Validated Antibody Database (VAD) review about zebrafish nkx6.1, based on 27 published articles (read how Labome selects the articles), using nkx6.1 antibody in all methods. It is aimed to help Labome visitors find the most suited nkx6.1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
nkx6.1 synonym: zgc:92879

Developmental Studies Hybridoma Bank
mouse monoclonal (F55A10)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2d
  • immunocytochemistry; human; fig 3c
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry - paraffin section on mouse samples (fig 2d) and in immunocytochemistry on human samples (fig 3c). Dev Cell (2022) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig s2m, s2u
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig s2m, s2u). Front Neurosci (2022) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; zebrafish ; 1:10; loading ...; fig 6s1a
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry on zebrafish samples at 1:10 (fig 6s1a). elife (2021) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; human; 1:250; fig 2d
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunocytochemistry on human samples at 1:250 (fig 2d). Diabetes (2021) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:100; loading ...; fig s2-1a
Developmental Studies Hybridoma Bank nkx6.1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples at 1:100 (fig s2-1a). elife (2020) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5l
  • chromatin immunoprecipitation; mouse; ; loading ...; fig 6e
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry - frozen section on mouse samples (fig 5l) and in chromatin immunoprecipitation on mouse samples at (fig 6e). Front Neurosci (2020) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:50; loading ...; fig 4d
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry on mouse samples at 1:50 (fig 4d). elife (2020) ncbi
mouse monoclonal (F55A10)
  • flow cytometry; human; loading ...; fig 7b
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in flow cytometry on human samples (fig 7b). Nat Commun (2019) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; human; fig 2a
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunocytochemistry on human samples (fig 2a). Cell (2018) ncbi
mouse monoclonal (F55A10)
  • flow cytometry; human; 1:2000; loading ...; tbl 1
In order to describe a four-stage differentiation protocol designed to generate pancreatic progenitor cells from human embryonic stem cells, Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in flow cytometry on human samples at 1:2000 (tbl 1). J Vis Exp (2017) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; mouse; fig 6
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry - frozen section on mouse samples (fig 6). Mol Cell Neurosci (2016) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; human; 1:300
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunocytochemistry on human samples at 1:300. Nature (2016) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:50; fig 1
In order to analyze regulation of formation of the columnar neural epithelium by the tumor suppressor PTEN and the PDK1 kinase, Developmental Studies Hybridoma Bank nkx6.1 antibody (Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples at 1:50 (fig 1). elife (2016) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; chicken; 1:20; fig 2
In order to assess and validate novel hedgehog-responsive enhancers by computational analysis prediction of Ci/Gli binding site density, Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry on chicken samples at 1:20 (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:50; fig 4
In order to characterize sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain by primary cilia, Developmental Studies Hybridoma Bank nkx6.1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples at 1:50 (fig 4). Dev Biol (2016) ncbi
mouse monoclonal (F55A10)
  • flow cytometry; human; 1:2000; fig 3
In order to describe protocols to generate specific pancreatic lineages from hPSCs, Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in flow cytometry on human samples at 1:2000 (fig 3). Methods (2016) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - paraffin section; human; 1:100; fig 3
In order to analyze a model for ductal pancreatic cancer and drug screening, Developmental Studies Hybridoma Bank nkx6.1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3). Nat Med (2015) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; human; 1:200; fig 4a
In order to demonstrate that dCas9 activator controls human pluripotent stem cell differentiation into endodermal lineages, Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunocytochemistry on human samples at 1:200 (fig 4a). Stem Cell Reports (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; human; 1:20; fig 1
In order to assess dorsal root avulsion injury and how human embryonic stem cell-derived progenitors assist functional sensory axo regeneration, Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry on human samples at 1:20 (fig 1). Sci Rep (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; fig s5
In order to study Shh signaling during oncogenesis and hindbrain development due to Eya1 phosphate promotion, Developmental Studies Hybridoma Bank nkx6.1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples (fig s5). Dev Cell (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:50; fig s5
In order to test if extracellular signals orient the mitotic spindle of cells in the spinal cord, Developmental Studies Hybridoma Bank nkx6.1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples at 1:50 (fig s5). Nat Commun (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 3
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 3). Nat Commun (2014) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; mouse; 1:100
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunocytochemistry on mouse samples at 1:100. Neurotoxicol Teratol (2014) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; chicken; 1:20
In order to study the role of Sox2 activity in neural differentiation, Developmental Studies Hybridoma Bank nkx6.1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - frozen section on chicken samples at 1:20. Proc Natl Acad Sci U S A (2014) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; chicken
Developmental Studies Hybridoma Bank nkx6.1 antibody (DSHB, F55A10) was used in immunohistochemistry - frozen section on chicken samples . PLoS ONE (2014) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - paraffin section; mouse
Developmental Studies Hybridoma Bank nkx6.1 antibody (Dev. Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - paraffin section on mouse samples . PLoS ONE (2013) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - free floating section; Spanish newt; 1:500
Developmental Studies Hybridoma Bank nkx6.1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - free floating section on Spanish newt samples at 1:500. J Comp Neurol (2013) ncbi
Articles Reviewed
  1. Miguel Escalada I, Maestro M, Balboa D, Elek A, Bernal A, Bernardo E, et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster. Dev Cell. 2022;57:1922-1936.e9 pubmed publisher
  2. Moore A, Chinnaiya K, Kim D, Brown S, Stewart I, Robins S, et al. Loss of Function of the Neural Cell Adhesion Molecule NrCAM Regulates Differentiation, Proliferation and Neurogenesis in Early Postnatal Hypothalamic Tanycytes. Front Neurosci. 2022;16:832961 pubmed publisher
  3. Geusz R, Wang A, Chiou J, Lancman J, Wetton N, Kefalopoulou S, et al. Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in development. elife. 2021;10: pubmed publisher
  4. Montaser H, Patel K, Balboa D, Ibrahim H, Lithovius V, Näätänen A, et al. Loss of MANF Causes Childhood-Onset Syndromic Diabetes Due to Increased Endoplasmic Reticulum Stress. Diabetes. 2021;70:1006-1018 pubmed publisher
  5. Yoshida S, Aoki K, Fujiwara K, Nakakura T, Kawamura A, Yamada K, et al. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis. elife. 2020;9: pubmed publisher
  6. Lozzi B, Huang T, Sardar D, Huang A, Deneen B. Regionally Distinct Astrocytes Display Unique Transcription Factor Profiles in the Adult Brain. Front Neurosci. 2020;14:61 pubmed publisher
  7. Gigante E, Taylor M, Ivanova A, Kahn R, Caspary T. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. elife. 2020;9: pubmed publisher
  8. Yung T, Poon F, Liang M, Coquenlorge S, McGaugh E, Hui C, et al. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals. Nat Commun. 2019;10:4647 pubmed publisher
  9. Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  10. McGaugh E, Nostro M. Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors. J Vis Exp. 2017;: pubmed publisher
  11. Fogarty L, Song B, Suppiah Y, Hasan S, Martin H, Hogan S, et al. Bcl-xL dependency coincides with the onset of neurogenesis in the developing mammalian spinal cord. Mol Cell Neurosci. 2016;77:34-46 pubmed publisher
  12. Sagi I, Chia G, Golan Lev T, Peretz M, Weissbein U, Sui L, et al. Derivation and differentiation of haploid human embryonic stem cells. Nature. 2016;532:107-11 pubmed publisher
  13. Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  14. Gurdziel K, Lorberbaum D, Udager A, Song J, Richards N, Parker D, et al. Identification and Validation of Novel Hedgehog-Responsive Enhancers Predicted by Computational Analysis of Ci/Gli Binding Site Density. PLoS ONE. 2015;10:e0145225 pubmed publisher
  15. Gazea M, Tasouri E, Tolve M, Bosch V, Kabanova A, Gojak C, et al. Primary cilia are critical for Sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain. Dev Biol. 2016;409:55-71 pubmed publisher
  16. Korytnikov R, Nostro M. Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells. Methods. 2016;101:56-64 pubmed publisher
  17. Huang L, Holtzinger A, Jagan I, BeGora M, Lohse I, Ngai N, et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med. 2015;21:1364-71 pubmed publisher
  18. Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015;5:448-59 pubmed publisher
  19. Hoeber J, Trolle C, König N, Du Z, Gallo A, Hermans E, et al. Human Embryonic Stem Cell-Derived Progenitors Assist Functional Sensory Axon Regeneration after Dorsal Root Avulsion Injury. Sci Rep. 2015;5:10666 pubmed publisher
  20. Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
  21. Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher
  22. Veleri S, Manjunath S, Fariss R, May Simera H, Brooks M, Foskett T, et al. Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis. Nat Commun. 2014;5:4207 pubmed publisher
  23. Akeju O, Davis Dusenbery B, Cassel S, Ichida J, Eggan K. Ketamine exposure in early development impairs specification of the primary germ cell layers. Neurotoxicol Teratol. 2014;43:59-68 pubmed publisher
  24. Lee K, Seo J, Shin J, Ji E, Roh J, Kim J, et al. Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system. Proc Natl Acad Sci U S A. 2014;111:2794-9 pubmed publisher
  25. Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
  26. Diaz de Durana Y, Lau J, Knee D, Filippi C, Londei M, McNamara P, et al. IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes. PLoS ONE. 2013;8:e78483 pubmed publisher
  27. Joven A, Morona R, González A, Moreno N. Spatiotemporal patterns of Pax3, Pax6, and Pax7 expression in the developing brain of a urodele amphibian, Pleurodeles waltl. J Comp Neurol. 2013;521:3913-53 pubmed publisher