This is a Validated Antibody Database (VAD) review about zebrafish acta2, based on 23 published articles (read how Labome selects the articles), using acta2 antibody in all methods. It is aimed to help Labome visitors find the most suited acta2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
acta2 synonym: wu:fb63d03; actin, aortic smooth muscle; sma

Invitrogen
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1a
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4c
In order to ask if meprins play a role in collagen maturation and lung fibrosis, Invitrogen acta2 antibody (Thermo Scientific, PA5-19465) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1a) and in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4c). Sci Rep (2017) ncbi
mouse monoclonal (mAbGEa)
  • western blot; baker's yeast; fig 2
In order to investigate the connection between calorie restriction and magnesium, Invitrogen acta2 antibody (Thermo Scientific, MA1-744) was used in western blot on baker's yeast samples (fig 2). Nucleic Acids Res (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; human; 1:500; loading ...; fig 1a
In order to make mutant mice to determine the impact of REV3L catalytic activity, Invitrogen acta2 antibody (Pierce, MA1-744) was used in western blot on human samples at 1:500 (fig 1a). DNA Repair (Amst) (2016) ncbi
mouse monoclonal (mAbGEa)
  • immunoprecipitation; rat; fig 2
In order to analyze the formation of supramolecular complexes through non-overlapping binding sites for drebrin, ZO-1, and tubulin by connexin43, Invitrogen acta2 antibody (Thermo scientific, MA1-744) was used in immunoprecipitation on rat samples (fig 2). PLoS ONE (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; thale cress; fig 1
In order to study the contribution to pattern-triggered immunity from the GSK3/Shaggy-like kinase ASKalpha, Invitrogen acta2 antibody (Thermo Scientific, MA1-744) was used in western blot on thale cress samples (fig 1). Plant Physiol (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; pig; loading ...; fig 2c
In order to test if adipose tissues have epigenetically distinct subpopulations of adipocytes, Invitrogen acta2 antibody (Thermo Scientific, mAbGEa) was used in western blot on pig samples (fig 2c). PLoS ONE (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; baker's yeast; 1:1000; fig 3
In order to regulating actin cable dynamics in budding yeast by fimbrin phosphorylation by metaphase Cdk1, Invitrogen acta2 antibody (Thermo Fisher scientific, mAbGEa) was used in western blot on baker's yeast samples at 1:1000 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; mouse; fig 2
In order to identify factors that are altered in the lacrimal gland by comparing several mouse models of disease with healthy mice, Invitrogen acta2 antibody (Thermo Scientific, mAbGEa) was used in western blot on mouse samples (fig 2). Invest Ophthalmol Vis Sci (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 7
In order to examine how the elasticity of the matrix affects the pluripotency of hPSCs, Invitrogen acta2 antibody (Life Technology, PA5-19465) was used in immunocytochemistry on human samples (fig 7). Sci Rep (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; baker's yeast; 1:1000; fig 2, 4
In order to report roles for kinesin and nuclear pore complexes in DNA repair by break-induced replication, Invitrogen acta2 antibody (Fisher, MA1-744) was used in western blot on baker's yeast samples at 1:1000 (fig 2, 4). Nat Commun (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; thale cress; 1:1000; fig 1
In order to distinguish the effects of photoreceptor signaling on clock function from those of photosynthesis, Invitrogen acta2 antibody (Thermo Scientific, MA1-744) was used in western blot on thale cress samples at 1:1000 (fig 1). Plant Physiol (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; human; 1:1000; fig 6
In order to examine the effects of neokestose on cell proliferation, cell cycle, and apoptosis of colonic cells, Invitrogen acta2 antibody (Thermo Fisher, MA1-744) was used in western blot on human samples at 1:1000 (fig 6). Mol Med Rep (2015) ncbi
mouse monoclonal (mAbGEa)
In order to characterize the Las17 G-actin-binding motif in vitro and in vivo, Invitrogen acta2 antibody (Fisher, MA1-744) was used . Traffic (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; human
In order to determine how HER2/HER3 regulates extracellular acidification and cell migration, Invitrogen acta2 antibody (Thermo Scientific, MA1-744) was used in western blot on human samples . Cell Signal (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
In order to investigate the effect of FGF2 in mesenchymal stem cells, Invitrogen acta2 antibody (Thermo Scientific Pierce, PA5-19465) was used in western blot on human samples at 1:1000. Mol Med Rep (2013) ncbi
mouse monoclonal (mAbGEa)
  • western blot; common platanna
In order to study metabolic regulation of CaMKII protein and caspases in Xenopus, Invitrogen acta2 antibody (Thermo Scientific, MA1-744) was used in western blot on common platanna samples . J Biol Chem (2013) ncbi
Abcam
mouse monoclonal (1A4)
  • flow cytometry; rat; loading ...; fig 7d
  • western blot; rat; loading ...; fig 3d
Abcam acta2 antibody (Abcam, ab8211) was used in flow cytometry on rat samples (fig 7d) and in western blot on rat samples (fig 3d). Oncotarget (2016) ncbi
mouse monoclonal (1A4)
  • immunocytochemistry; mouse; 1:100
Abcam acta2 antibody (abcam, ab8211) was used in immunocytochemistry on mouse samples at 1:100. Cancer Res (2015) ncbi
mouse monoclonal (1A4)
Abcam acta2 antibody (Abcam, ab8211) was used . Front Oncol (2015) ncbi
mouse monoclonal (1A4)
  • western blot; human
In order to discuss the contribution of missense mutations in PDE3A to hypertension, Abcam acta2 antibody (Abcam, ab8211) was used in western blot on human samples . Nat Genet (2015) ncbi
mouse monoclonal (1A4)
  • immunohistochemistry - paraffin section; mouse
Abcam acta2 antibody (Abcam, ab8211) was used in immunohistochemistry - paraffin section on mouse samples . Cardiovasc Res (2015) ncbi
mouse monoclonal (1A4)
  • flow cytometry; human
Abcam acta2 antibody (Abcam, ab8211) was used in flow cytometry on human samples . Am J Pathol (2015) ncbi
rabbit polyclonal
  • western blot; human
Abcam acta2 antibody (Abcam, ab15734) was used in western blot on human samples . PLoS ONE (2014) ncbi
Articles Reviewed
  1. Biasin V, Wygrecka M, Marsh L, Becker Pauly C, Brcic L, Ghanim B, et al. Meprin β contributes to collagen deposition in lung fibrosis. Sci Rep. 2017;7:39969 pubmed publisher
  2. Feng W, Zhang K, Liu Y, Chen J, Cai Q, Zhang Y, et al. Apocynin attenuates angiotensin II-induced vascular smooth muscle cells osteogenic switching via suppressing extracellular signal-regulated kinase 1/2. Oncotarget. 2016;7:83588-83600 pubmed publisher
  3. Abraham K, Chan J, Salvi J, Ho B, Hall A, Vidya E, et al. Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acids Res. 2016;44:8870-8884 pubmed
  4. Fritzen R, Delbos F, De Smet A, Palancade B, Canman C, Aoufouchi S, et al. A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro. DNA Repair (Amst). 2016;46:37-46 pubmed publisher
  5. Ambrosi C, Ren C, Spagnol G, Cavin G, CONE A, Grintsevich E, et al. Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1. PLoS ONE. 2016;11:e0157073 pubmed publisher
  6. Stampfl H, Fritz M, Dal Santo S, Jonak C. The GSK3/Shaggy-Like Kinase ASKα Contributes to Pattern-Triggered Immunity. Plant Physiol. 2016;171:1366-77 pubmed publisher
  7. Yu P, Ji L, Lee K, Yu M, He C, Ambati S, et al. Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine. PLoS ONE. 2016;11:e0154949 pubmed publisher
  8. Miao Y, Han X, Zheng L, Xie Y, Mu Y, Yates J, et al. Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast. Nat Commun. 2016;7:11265 pubmed publisher
  9. Umazume T, Thomas W, Campbell S, Aluri H, Thotakura S, Zoukhri D, et al. Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells. Invest Ophthalmol Vis Sci. 2015;56:8392-402 pubmed publisher
  10. Higuchi A, Kao S, Ling Q, Chen Y, Li H, Alarfaj A, et al. Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. Sci Rep. 2015;5:18136 pubmed publisher
  11. Chung D, Chan J, Strecker J, Zhang W, Ebrahimi Ardebili S, Lu T, et al. Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process. Nat Commun. 2015;6:7742 pubmed publisher
  12. Jones M, Hu W, Litthauer S, Lagarias J, Harmer S. A Constitutively Active Allele of Phytochrome B Maintains Circadian Robustness in the Absence of Light. Plant Physiol. 2015;169:814-25 pubmed publisher
  13. Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala Dilke K, Leinster D, et al. Interleukin-6 Stimulates Defective Angiogenesis. Cancer Res. 2015;75:3098-107 pubmed publisher
  14. Gorchs L, Hellevik T, Bruun J, Camilio K, Al Saad S, Stuge T, et al. Cancer-associated fibroblasts from lung tumors maintain their immunosuppressive abilities after high-dose irradiation. Front Oncol. 2015;5:87 pubmed publisher
  15. Maass P, Aydin A, Luft F, Schächterle C, Weise A, Stricker S, et al. PDE3A mutations cause autosomal dominant hypertension with brachydactyly. Nat Genet. 2015;47:647-53 pubmed publisher
  16. Wan W, Liu Q, Lionakis M, Marino A, Anderson S, Swamydas M, et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res. 2015;106:478-87 pubmed publisher
  17. Lee S, Chang J, Wu J, Sheu D. Antineoplastic effect of a novel chemopreventive agent, neokestose, on the Caco-2 cell line via inhibition of expression of nuclear factor-κB and cyclooxygenase-2. Mol Med Rep. 2015;12:1114-8 pubmed publisher
  18. Feliciano D, Tolsma T, Farrell K, Aradi A, Di Pietro S. A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis. Traffic. 2015;16:379-97 pubmed publisher
  19. Yuan K, Orcholski M, Panaroni C, Shuffle E, Huang N, Jiang X, et al. Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis. Am J Pathol. 2015;185:69-84 pubmed publisher
  20. Hu B, Song J, Qu H, Bi C, Huang X, Liu X, et al. Mechanical stretch suppresses microRNA-145 expression by activating extracellular signal-regulated kinase 1/2 and upregulating angiotensin-converting enzyme to alter vascular smooth muscle cell phenotype. PLoS ONE. 2014;9:e96338 pubmed publisher
  21. Sollome J, Thavathiru E, Camenisch T, Vaillancourt R. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal. 2014;26:70-82 pubmed publisher
  22. Cai T, Zhu W, Chen X, Zhou S, Jia L, Sun Y. Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway. Mol Med Rep. 2013;8:1323-8 pubmed publisher
  23. McCoy F, Darbandi R, Chen S, Eckard L, Dodd K, Jones K, et al. Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts. J Biol Chem. 2013;288:8838-48 pubmed publisher