This is a Validated Antibody Database (VAD) review about zebrafish acta1a, based on 31 published articles (read how Labome selects the articles), using acta1a antibody in all methods. It is aimed to help Labome visitors find the most suited acta1a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
acta1a synonym: hm:zeh0293; zgc:112098; actin, gamma-enteric smooth muscle; zeh0293

Invitrogen
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4c
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1a
In order to ask if meprins play a role in collagen maturation and lung fibrosis, Invitrogen acta1a antibody (Thermo Scientific, PA5-19465) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4c) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1a). Sci Rep (2017) ncbi
mouse monoclonal (mAbGEa)
  • western blot; baker's yeast; fig 2
In order to investigate the connection between calorie restriction and magnesium, Invitrogen acta1a antibody (Thermo Scientific, MA1-744) was used in western blot on baker's yeast samples (fig 2). Nucleic Acids Res (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; human; 1:500; loading ...; fig 1a
In order to make mutant mice to determine the impact of REV3L catalytic activity, Invitrogen acta1a antibody (Pierce, MA1-744) was used in western blot on human samples at 1:500 (fig 1a). DNA Repair (Amst) (2016) ncbi
mouse monoclonal (mAbGEa)
  • immunoprecipitation; rat; fig 2
In order to analyze the formation of supramolecular complexes through non-overlapping binding sites for drebrin, ZO-1, and tubulin by connexin43, Invitrogen acta1a antibody (Thermo scientific, MA1-744) was used in immunoprecipitation on rat samples (fig 2). PLoS ONE (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; thale cress; fig 1
In order to study the contribution to pattern-triggered immunity from the GSK3/Shaggy-like kinase ASKalpha, Invitrogen acta1a antibody (Thermo Scientific, MA1-744) was used in western blot on thale cress samples (fig 1). Plant Physiol (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; pig; loading ...; fig 2c
In order to test if adipose tissues have epigenetically distinct subpopulations of adipocytes, Invitrogen acta1a antibody (Thermo Scientific, mAbGEa) was used in western blot on pig samples (fig 2c). PLoS ONE (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; baker's yeast; 1:1000; fig 3
In order to regulating actin cable dynamics in budding yeast by fimbrin phosphorylation by metaphase Cdk1, Invitrogen acta1a antibody (Thermo Fisher scientific, mAbGEa) was used in western blot on baker's yeast samples at 1:1000 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (mAbGEa)
  • western blot; mouse; fig 2
In order to identify factors that are altered in the lacrimal gland by comparing several mouse models of disease with healthy mice, Invitrogen acta1a antibody (Thermo Scientific, mAbGEa) was used in western blot on mouse samples (fig 2). Invest Ophthalmol Vis Sci (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 7
In order to examine how the elasticity of the matrix affects the pluripotency of hPSCs, Invitrogen acta1a antibody (Life Technology, PA5-19465) was used in immunocytochemistry on human samples (fig 7). Sci Rep (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; baker's yeast; 1:1000; fig 2, 4
In order to report roles for kinesin and nuclear pore complexes in DNA repair by break-induced replication, Invitrogen acta1a antibody (Fisher, MA1-744) was used in western blot on baker's yeast samples at 1:1000 (fig 2, 4). Nat Commun (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; thale cress; 1:1000; fig 1
In order to distinguish the effects of photoreceptor signaling on clock function from those of photosynthesis, Invitrogen acta1a antibody (Thermo Scientific, MA1-744) was used in western blot on thale cress samples at 1:1000 (fig 1). Plant Physiol (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; human; 1:1000; fig 6
In order to examine the effects of neokestose on cell proliferation, cell cycle, and apoptosis of colonic cells, Invitrogen acta1a antibody (Thermo Fisher, MA1-744) was used in western blot on human samples at 1:1000 (fig 6). Mol Med Rep (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; scFv
In order to characterize the Las17 G-actin-binding motif in vitro and in vivo, Invitrogen acta1a antibody (Fisher, MA1-744) was used in western blot on scFv samples . Traffic (2015) ncbi
mouse monoclonal (mAbGEa)
  • western blot; human
In order to determine how HER2/HER3 regulates extracellular acidification and cell migration, Invitrogen acta1a antibody (Thermo Scientific, MA1-744) was used in western blot on human samples . Cell Signal (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
In order to investigate the effect of FGF2 in mesenchymal stem cells, Invitrogen acta1a antibody (Thermo Scientific Pierce, PA5-19465) was used in western blot on human samples at 1:1000. Mol Med Rep (2013) ncbi
mouse monoclonal (mAbGEa)
  • western blot; common platanna
In order to study metabolic regulation of CaMKII protein and caspases in Xenopus, Invitrogen acta1a antibody (Thermo Scientific, MA1-744) was used in western blot on common platanna samples . J Biol Chem (2013) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (JLA20)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 2n
Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2n). Neuron (2018) ncbi
mouse monoclonal (JLA20)
  • immunocytochemistry; mouse; loading ...; fig 3a
  • western blot; mouse; 1:2000; loading ...; fig 2a
Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in immunocytochemistry on mouse samples (fig 3a) and in western blot on mouse samples at 1:2000 (fig 2a). J Neurosci (2018) ncbi
mouse monoclonal (JLA20)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4a
Developmental Studies Hybridoma Bank acta1a antibody (DSHB, JLA-20) was used in immunohistochemistry - frozen section on mouse samples (fig 4a). J Cell Biol (2016) ncbi
mouse monoclonal (JLA20)
  • western blot; mouse; fig 1
In order to analyze the mitigation that increases PHF1 levels and lipofuscin puncta and rescues memory deficits by TFEB overexpression in the P301S model of tauopathy, Developmental Studies Hybridoma Bank acta1a antibody (DSHB, JLA20) was used in western blot on mouse samples (fig 1). Eneuro (2016) ncbi
mouse monoclonal (JLA20)
  • western blot; mouse; fig 2e
In order to analyze colitis and colon carcinogenesis and the role of sestrin2 in suppressing tumors, Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in western blot on mouse samples (fig 2e). elife (2016) ncbi
mouse monoclonal (JLA20)
  • western blot; human; fig 3
Developmental Studies Hybridoma Bank acta1a antibody (DSHB, JLA20) was used in western blot on human samples (fig 3). elife (2016) ncbi
mouse monoclonal (JLA20)
  • western blot; fruit fly; 1:2000; fig 1F
Developmental Studies Hybridoma Bank acta1a antibody (DSHB, JLA20) was used in western blot on fruit fly samples at 1:2000 (fig 1F). Dis Model Mech (2016) ncbi
mouse monoclonal (JLA20)
  • western blot; fruit fly; 1:50
In order to develop a Drosophila model for Charcot-Marie-Tooth by mutating glycyl-tRNA synthetase, Developmental Studies Hybridoma Bank acta1a antibody (DSHB, JLA20) was used in western blot on fruit fly samples at 1:50. Nat Commun (2015) ncbi
mouse monoclonal (JLA20)
  • western blot; human; 1:4000; fig 1, 2
Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in western blot on human samples at 1:4000 (fig 1, 2). J Immunol (2015) ncbi
mouse monoclonal (JLA20)
  • western blot; fruit fly; 1:1000; fig 1
Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in western blot on fruit fly samples at 1:1000 (fig 1). Proc Natl Acad Sci U S A (2014) ncbi
mouse monoclonal (JLA20)
  • western blot; common platanna; 1:300; fig 3
In order to examine directed cell migration within an epithelial tissue, Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in western blot on common platanna samples at 1:300 (fig 3). J Cell Biol (2014) ncbi
mouse monoclonal (JLA20)
  • western blot; fruit fly; 1:5000
Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in western blot on fruit fly samples at 1:5000. Hum Mol Genet (2014) ncbi
mouse monoclonal (JLA20)
  • western blot; mouse; fig 5c
Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in western blot on mouse samples (fig 5c). Hum Mol Genet (2014) ncbi
mouse monoclonal (JLA20)
  • western blot; human
Developmental Studies Hybridoma Bank acta1a antibody (DSHB, JLA20) was used in western blot on human samples . PLoS ONE (2013) ncbi
mouse monoclonal (JLA20)
  • western blot; fruit fly; 1:10
Developmental Studies Hybridoma Bank acta1a antibody (Developmental Studies Hybridoma Bank, JLA20) was used in western blot on fruit fly samples at 1:10. Proteomics (2011) ncbi
Articles Reviewed
  1. Ordonez D, Lee M, Feany M. α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton. Neuron. 2018;97:108-124.e6 pubmed publisher
  2. Krey J, Dumont R, Wilmarth P, David L, Johnson K, Barr Gillespie P. ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells. J Neurosci. 2018;38:843-857 pubmed publisher
  3. Biasin V, Wygrecka M, Marsh L, Becker Pauly C, Brcic L, Ghanim B, et al. Meprin β contributes to collagen deposition in lung fibrosis. Sci Rep. 2017;7:39969 pubmed publisher
  4. Krey J, Krystofiak E, Dumont R, Vijayakumar S, Choi D, Rivero F, et al. Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid. J Cell Biol. 2016;215:467-482 pubmed
  5. Abraham K, Chan J, Salvi J, Ho B, Hall A, Vidya E, et al. Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acids Res. 2016;44:8870-8884 pubmed
  6. Fritzen R, Delbos F, De Smet A, Palancade B, Canman C, Aoufouchi S, et al. A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro. DNA Repair (Amst). 2016;46:37-46 pubmed publisher
  7. Ambrosi C, Ren C, Spagnol G, Cavin G, CONE A, Grintsevich E, et al. Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1. PLoS ONE. 2016;11:e0157073 pubmed publisher
  8. Wang H, Wang R, Carrera I, Xu S, Lakshmana M. TFEB Overexpression in the P301S Model of Tauopathy Mitigates Increased PHF1 Levels and Lipofuscin Puncta and Rescues Memory Deficits. Eneuro. 2016;3: pubmed publisher
  9. Stampfl H, Fritz M, Dal Santo S, Jonak C. The GSK3/Shaggy-Like Kinase ASKα Contributes to Pattern-Triggered Immunity. Plant Physiol. 2016;171:1366-77 pubmed publisher
  10. Yu P, Ji L, Lee K, Yu M, He C, Ambati S, et al. Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine. PLoS ONE. 2016;11:e0154949 pubmed publisher
  11. Miao Y, Han X, Zheng L, Xie Y, Mu Y, Yates J, et al. Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast. Nat Commun. 2016;7:11265 pubmed publisher
  12. Ro S, Xue X, Ramakrishnan S, Cho C, Namkoong S, Jang I, et al. Tumor suppressive role of sestrin2 during colitis and colon carcinogenesis. elife. 2016;5:e12204 pubmed publisher
  13. Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, et al. Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay. elife. 2016;5: pubmed publisher
  14. Umazume T, Thomas W, Campbell S, Aluri H, Thotakura S, Zoukhri D, et al. Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells. Invest Ophthalmol Vis Sci. 2015;56:8392-402 pubmed publisher
  15. Lee S, Bang S, Hong Y, Lee J, Jeong H, Park S, et al. The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease. Dis Model Mech. 2016;9:295-306 pubmed publisher
  16. Higuchi A, Kao S, Ling Q, Chen Y, Li H, Alarfaj A, et al. Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. Sci Rep. 2015;5:18136 pubmed publisher
  17. Chung D, Chan J, Strecker J, Zhang W, Ebrahimi Ardebili S, Lu T, et al. Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process. Nat Commun. 2015;6:7742 pubmed publisher
  18. Jones M, Hu W, Litthauer S, Lagarias J, Harmer S. A Constitutively Active Allele of Phytochrome B Maintains Circadian Robustness in the Absence of Light. Plant Physiol. 2015;169:814-25 pubmed publisher
  19. Niehues S, Bussmann J, Steffes G, Erdmann I, Köhrer C, Sun L, et al. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun. 2015;6:7520 pubmed publisher
  20. Lee S, Chang J, Wu J, Sheu D. Antineoplastic effect of a novel chemopreventive agent, neokestose, on the Caco-2 cell line via inhibition of expression of nuclear factor-κB and cyclooxygenase-2. Mol Med Rep. 2015;12:1114-8 pubmed publisher
  21. Cuadrado E, Michailidou I, van Bodegraven E, Jansen M, Sluijs J, Geerts D, et al. Phenotypic variation in Aicardi-Goutières syndrome explained by cell-specific IFN-stimulated gene response and cytokine release. J Immunol. 2015;194:3623-33 pubmed publisher
  22. Feliciano D, Tolsma T, Farrell K, Aradi A, Di Pietro S. A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis. Traffic. 2015;16:379-97 pubmed publisher
  23. Merlo P, Frost B, Peng S, Yang Y, Park P, Feany M. p53 prevents neurodegeneration by regulating synaptic genes. Proc Natl Acad Sci U S A. 2014;111:18055-60 pubmed publisher
  24. Werner M, Mitchell J, Putzbach W, Bacon E, Kim S, Mitchell B. Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1. J Cell Biol. 2014;206:367-76 pubmed publisher
  25. Hegde V, Vogel R, Feany M. Glia are critical for the neuropathology of complex I deficiency in Drosophila. Hum Mol Genet. 2014;23:4686-92 pubmed publisher
  26. Wang H, Yang B, Qiu L, Yang C, Kramer J, Su Q, et al. Widespread spinal cord transduction by intrathecal injection of rAAV delivers efficacious RNAi therapy for amyotrophic lateral sclerosis. Hum Mol Genet. 2014;23:668-81 pubmed publisher
  27. Sollome J, Thavathiru E, Camenisch T, Vaillancourt R. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal. 2014;26:70-82 pubmed publisher
  28. Cai T, Zhu W, Chen X, Zhou S, Jia L, Sun Y. Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway. Mol Med Rep. 2013;8:1323-8 pubmed publisher
  29. Pe er T, Lahmi R, Sharaby Y, Chorni E, Noach M, Vecsler M, et al. Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex. PLoS ONE. 2013;8:e57532 pubmed publisher
  30. McCoy F, Darbandi R, Chen S, Eckard L, Dodd K, Jones K, et al. Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts. J Biol Chem. 2013;288:8838-48 pubmed publisher
  31. Koppen T, Weckmann A, Muller S, Staubach S, Bloch W, Dohmen R, et al. Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics. 2011;11:4397-410 pubmed publisher