This is a Validated Antibody Database (VAD) review about sheep STAT5A, based on 24 published articles (read how Labome selects the articles), using STAT5A antibody in all methods. It is aimed to help Labome visitors find the most suited STAT5A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
STAT5A synonym: MGF; STAT5; signal transducer and activator of transcription 5A; mammary gland factor; signal transducer and activator of transcription 5

Invitrogen
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4g
  • western blot; mouse; 1:1000; loading ...; fig 4a
In order to investigate estrogen receptor alpha positive breast cancer, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4g) and in western blot on mouse samples at 1:1000 (fig 4a). Breast Cancer Res (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1a
In order to determine the molecular mechanisms that enable JAK2/signal transducer and activator of transcription 5 to access promoters of genes implicated in breast cancer pathogenesis, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in western blot on human samples (fig 1a). J Biol Chem (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 2c
In order to explore the extracellular matrix features that control prolactin signals, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in western blot on human samples (fig 2c). Oncotarget (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:100
In order to examine the daily rhythm of STAT3 protein, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in immunohistochemistry - free floating section on rat samples at 1:100. J Neurosci Res (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 5
  • western blot; mouse; 1:10000; fig 5
In order to elucidate the molecular mechanisms involved in the upregulation of ABCG2 during lactation, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 5) and in western blot on mouse samples at 1:10000 (fig 5). Am J Physiol Endocrinol Metab (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
In order to study the regulation of growth hormone stimulated genes, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in chromatin immunoprecipitation on mouse samples . Mol Cell Endocrinol (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse; 1:1000
In order to report that image analysis decreased inter-observer variability when scoring immunohistologically stained human hepatocellular patient samples, Invitrogen STAT5A antibody (Invitrogen, #71-6900) was used in immunohistochemistry - paraffin section on mouse samples and in western blot on mouse samples at 1:1000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; human
In order to study the involvement of collagen matrices in tumorigenic prolactin signaling in breast cancer cells, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in western blot on human samples . J Biol Chem (2013) ncbi
rabbit polyclonal
  • western blot; human; 1:4000; fig 3
In order to elucidate the role of the lactogenic hormone prolactin in the regulation of ABCG2, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in western blot on human samples at 1:4000 (fig 3). Mol Pharmacol (2013) ncbi
rabbit polyclonal
  • western blot; mouse; fig 2
In order to determine the roles of growth hormone and STAT5 in metabolism and liver cancer onset, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in western blot on mouse samples (fig 2). Hepatology (2011) ncbi
rabbit polyclonal
  • western blot; mouse; fig 7
In order to test if Jak2 contributes to Ang II-mediated neointima formation following vascular injury, Invitrogen STAT5A antibody (Invitrogen, 716900) was used in western blot on mouse samples (fig 7). J Mol Cell Cardiol (2011) ncbi
rabbit polyclonal
  • western blot; human; fig 1
In order to study the role of Stat5 serine phosphorylation in oncogenesis, Invitrogen STAT5A antibody (Zymed, 71-6900) was used in western blot on human samples (fig 1). Blood (2010) ncbi
rabbit polyclonal
  • western blot; mouse
In order to determine the role of STAT5 in liver fibrogenesis, Invitrogen STAT5A antibody (Invitrogen, 71-6900) was used in western blot on mouse samples . Hepatology (2010) ncbi
rabbit polyclonal
  • western blot; mouse; fig 2
In order to determine the extent to which JAK2 and Src family kinases mediate GH-mediated activation of STAT1, 3, and 5a/b, ERKs 1 and 2, and Akt, Invitrogen STAT5A antibody (Zymed Laboratories, 71-6900) was used in western blot on mouse samples (fig 2). Mol Endocrinol (2008) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; fig 8b
  • western blot; mouse; fig 8a
In order to assess the role of FAK in mammary epithelial cells in vivo, Invitrogen STAT5A antibody (Zymed Laboratories, 71-6900) was used in immunohistochemistry on mouse samples (fig 8b) and in western blot on mouse samples (fig 8a). J Biol Chem (2007) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3A
In order to examine the consequences of Stat5 crosstalk with the glucocorticoid receptor in hepatocytes, Invitrogen STAT5A antibody (Zymed, 71-6900) was used in western blot on mouse samples (fig 3A). Genes Dev (2007) ncbi
rabbit polyclonal
  • western blot; human
In order to examine STAT3 following resistance exercise in human skeletal muscle, Invitrogen STAT5A antibody (Zymed, 71-6900) was used in western blot on human samples . J Appl Physiol (1985) (2007) ncbi
rabbit polyclonal
  • EMSA; human
In order to propose that Stat5 tetramers are associated with leukemogenesis, Invitrogen STAT5A antibody (Zymed, 71-6900) was used in EMSA on human samples . Cancer Cell (2005) ncbi
rabbit polyclonal
  • western blot; human
In order to assess GH receptor expression and signal transduction and the effect of GH and IGF-I exposure on cell proliferation and hormone secretion in nonfunctioning pituitary adenomas, Invitrogen STAT5A antibody (ZYMED, 71-6900) was used in western blot on human samples . Clin Endocrinol (Oxf) (2004) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:40
In order to examine the expression, tyrosine phosphorylation status and nuclear localization of Stat5a in human breast cancers, Invitrogen STAT5A antibody (Zymed, 71-6900) was used in immunohistochemistry - paraffin section on human samples at 1:40. Int J Cancer (2004) ncbi
rabbit polyclonal
  • western blot; mouse
In order to study T cell responses in the presence of IL-2 and IL-15, Invitrogen STAT5A antibody (Zymed, 71-6900) was used in western blot on mouse samples . Proc Natl Acad Sci U S A (2003) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4
In order to test if a mimic of phosphorylated human prolactin, S179D-PRL, initiates signaling in mammary tumor cells alone and in combination with unmodified prolactin, Invitrogen STAT5A antibody (Zymed, 71-6900) was used in western blot on human samples at 1:1000 (fig 4). Endocrinology (2003) ncbi
rabbit polyclonal
  • western blot; mouse; 1:50; fig 5c
In order to study the role of p21-activated kinase in the mammary gland, Invitrogen STAT5A antibody (Zymed, 71?C6900) was used in western blot on mouse samples at 1:50 (fig 5c). J Cell Biol (2003) ncbi
rabbit polyclonal
  • western blot; mouse; 1 ug/ml; fig 5
In order to investigate the role of STATs and PRL receptors in TGFalpha-induced mammary epithelial abnormalities in vivo, Invitrogen STAT5A antibody (Zymed Labs, 71-6900) was used in western blot on mouse samples at 1 ug/ml (fig 5). Mol Cell Endocrinol (2001) ncbi
Articles Reviewed
  1. Barcus C, O Leary K, Brockman J, Rugowski D, Liu Y, Garcia N, et al. Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells. Breast Cancer Res. 2017;19:9 pubmed publisher
  2. Schauwecker S, Kim J, Licht J, Clevenger C. Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells. J Biol Chem. 2017;292:2237-2254 pubmed publisher
  3. Barcus C, Keely P, Eliceiri K, Schuler L. Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells. Oncotarget. 2016;7:48093-48106 pubmed publisher
  4. Moravcová S, Červená K, Pačesová D, Bendová Z. Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide. J Neurosci Res. 2016;94:99-108 pubmed publisher
  5. Wu A, Yang M, Dalvi P, Turinsky A, Wang W, Butcher D, et al. Role of STAT5 and epigenetics in lactation-associated upregulation of multidrug transporter ABCG2 in the mammary gland. Am J Physiol Endocrinol Metab. 2014;307:E596-610 pubmed publisher
  6. Lin G, LaPensee C, Qin Z, Schwartz J. Reciprocal occupancy of BCL6 and STAT5 on Growth Hormone target genes: contrasting transcriptional outcomes and promoter-specific roles of p300 and HDAC3. Mol Cell Endocrinol. 2014;395:19-31 pubmed publisher
  7. Schlederer M, Mueller K, Haybaeck J, Heider S, Huttary N, Rosner M, et al. Reliable quantification of protein expression and cellular localization in histological sections. PLoS ONE. 2014;9:e100822 pubmed publisher
  8. Barcus C, Keely P, Eliceiri K, Schuler L. Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells. J Biol Chem. 2013;288:12722-32 pubmed publisher
  9. Wu A, Dalvi P, Lu X, Yang M, Riddick D, Matthews J, et al. Induction of multidrug resistance transporter ABCG2 by prolactin in human breast cancer cells. Mol Pharmacol. 2013;83:377-88 pubmed publisher
  10. Mueller K, Kornfeld J, Friedbichler K, Blaas L, Egger G, Esterbauer H, et al. Impairment of hepatic growth hormone and glucocorticoid receptor signaling causes steatosis and hepatocellular carcinoma in mice. Hepatology. 2011;54:1398-409 pubmed publisher
  11. Kirabo A, Oh S, Kasahara H, Wagner K, Sayeski P. Vascular smooth muscle Jak2 deletion prevents angiotensin II-mediated neointima formation following injury in mice. J Mol Cell Cardiol. 2011;50:1026-34 pubmed publisher
  12. Friedbichler K, Kerenyi M, Kovacic B, Li G, Hoelbl A, Yahiaoui S, et al. Stat5a serine 725 and 779 phosphorylation is a prerequisite for hematopoietic transformation. Blood. 2010;116:1548-58 pubmed publisher
  13. Blaas L, Kornfeld J, Schramek D, Musteanu M, Zollner G, Gumhold J, et al. Disruption of the growth hormone--signal transducer and activator of transcription 5--insulinlike growth factor 1 axis severely aggravates liver fibrosis in a mouse model of cholestasis. Hepatology. 2010;51:1319-26 pubmed publisher
  14. Jin H, Lanning N, Carter Su C. JAK2, but not Src family kinases, is required for STAT, ERK, and Akt signaling in response to growth hormone in preadipocytes and hepatoma cells. Mol Endocrinol. 2008;22:1825-41 pubmed publisher
  15. Nagy T, Wei H, Shen T, Peng X, Liang C, Gan B, et al. Mammary epithelial-specific deletion of the focal adhesion kinase gene leads to severe lobulo-alveolar hypoplasia and secretory immaturity of the murine mammary gland. J Biol Chem. 2007;282:31766-76 pubmed
  16. Engblom D, Kornfeld J, Schwake L, Tronche F, Reimann A, Beug H, et al. Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression. Genes Dev. 2007;21:1157-62 pubmed
  17. Trenerry M, Carey K, Ward A, Cameron Smith D. STAT3 signaling is activated in human skeletal muscle following acute resistance exercise. J Appl Physiol (1985). 2007;102:1483-9 pubmed
  18. Moriggl R, Sexl V, Kenner L, Duntsch C, Stangl K, Gingras S, et al. Stat5 tetramer formation is associated with leukemogenesis. Cancer Cell. 2005;7:87-99 pubmed
  19. Clausen L, Kristiansen M, Rasmussen L, Billestrup N, Blaabjerg O, Ledet T, et al. Growth hormone receptor expression and function in pituitary adenomas. Clin Endocrinol (Oxf). 2004;60:576-83 pubmed
  20. Cotarla I, Ren S, Zhang Y, Gehan E, Singh B, Furth P. Stat5a is tyrosine phosphorylated and nuclear localized in a high proportion of human breast cancers. Int J Cancer. 2004;108:665-71 pubmed
  21. Dubois S, Shou W, Haneline L, Fleischer S, Waldmann T, Müller J. Distinct pathways involving the FK506-binding proteins 12 and 12.6 underlie IL-2-versus IL-15-mediated proliferation of T cells. Proc Natl Acad Sci U S A. 2003;100:14169-74 pubmed
  22. Schroeder M, Brockman J, Walker A, Schuler L. Inhibition of prolactin (PRL)-induced proliferative signals in breast cancer cells by a molecular mimic of phosphorylated PRL, S179D-PRL. Endocrinology. 2003;144:5300-7 pubmed
  23. Wang R, Vadlamudi R, Bagheri Yarmand R, Beuvink I, Hynes N, Kumar R. Essential functions of p21-activated kinase 1 in morphogenesis and differentiation of mammary glands. J Cell Biol. 2003;161:583-92 pubmed
  24. Schroeder M, Rose Hellekant T, Sandgren E, Schuler L. Dysregulation of mammary Stats 1,3 and 5 and PRL receptors by overexpression of TGFalpha. Mol Cell Endocrinol. 2001;175:173-83 pubmed