This is a Validated Antibody Database (VAD) review about sheep NR3C1, based on 11 published articles (read how Labome selects the articles), using NR3C1 antibody in all methods. It is aimed to help Labome visitors find the most suited NR3C1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NR3C1 synonym: GRL; glucocorticoid receptor; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Invitrogen
mouse monoclonal (BuGR2)
  • immunohistochemistry - frozen section; rat; loading ...; fig 4a
In order to study the involvement of corticosterone in orofacial mechanical hypersensitivity induced by maternal separation, Invitrogen NR3C1 antibody (ThermoFisher Scientific, MA1-510) was used in immunohistochemistry - frozen section on rat samples (fig 4a). J Dent Res (2016) ncbi
mouse monoclonal (BuGR2)
  • western blot; mouse; 1:6000; fig 3
In order to research induction of robust depressive-like behavior and reduced BDNF levels in mice due to a disruption of the HPA-axis through corticosterone-release pellets, Invitrogen NR3C1 antibody (Thermo Scientific, MA1-510) was used in western blot on mouse samples at 1:6000 (fig 3). Neurosci Lett (2016) ncbi
mouse monoclonal (BuGR2)
  • chromatin immunoprecipitation; mouse; fig 1d
  • western blot; mouse; fig s3c
In order to show how forkhead box A3 mediates glucocorticoid receptor function in adipose tissue, Invitrogen NR3C1 antibody (Thermo Scientific, MA1-510) was used in chromatin immunoprecipitation on mouse samples (fig 1d) and in western blot on mouse samples (fig s3c). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (BuGR2)
  • immunohistochemistry - paraffin section; human
In order to establish an immunohistochemistry assay to examine glucocorticoid receptor expression in human invasive breast carcinoma samples, Invitrogen NR3C1 antibody (Thermo Fisher Scientific, MA1-510) was used in immunohistochemistry - paraffin section on human samples . Cancer Manag Res (2015) ncbi
mouse monoclonal (BuGR2)
  • chromatin immunoprecipitation; rat; fig 2
In order to study the molecular mechanism underlying neurotrophic-priming of glucocorticoid receptor function, Invitrogen NR3C1 antibody (Thermo Scientific, MA1-510) was used in chromatin immunoprecipitation on rat samples (fig 2). Proc Natl Acad Sci U S A (2015) ncbi
mouse monoclonal (BuGR2)
  • chromatin immunoprecipitation; mouse
In order to study the effect of pulsatile hormone stimulations on gene and regulatory element activation, Invitrogen NR3C1 antibody (ABR, MA1-510) was used in chromatin immunoprecipitation on mouse samples . Genome Res (2015) ncbi
mouse monoclonal (BuGR2)
  • immunohistochemistry; sheep; 1:50
  • western blot; sheep; 1:1000
In order to measure the expression of glucocorticoid receptors in pregnant ewes, Invitrogen NR3C1 antibody (Thermo Scientific, MA1-510) was used in immunohistochemistry on sheep samples at 1:50 and in western blot on sheep samples at 1:1000. Reprod Sci (2015) ncbi
mouse monoclonal (BuGR2)
  • western blot; rat; 1:1000
In order to study the effect of environment enrichment on maternal immune activation, Invitrogen NR3C1 antibody (Pierce, MA1-510) was used in western blot on rat samples at 1:1000. Brain Behav Immun (2014) ncbi
mouse monoclonal (BuGR2)
  • chromatin immunoprecipitation; rat
  • chromatin immunoprecipitation; human; 6 ug/time
In order to study the role of glucocorticoid receptor phosphorylation in the mechanism by which BDNF modulates the glucocorticoid transcriptome, Invitrogen NR3C1 antibody (Thermo Scientific, MA1-510) was used in chromatin immunoprecipitation on rat samples and in chromatin immunoprecipitation on human samples at 6 ug/time. Mol Cell Biol (2013) ncbi
mouse monoclonal (BuGR2)
  • western blot; rat
  • western blot; human; fig 1d
In order to use a novel competition assay to study the spatial and temporal binding of cofactors and reporter genes involved in steroid-regulated transactivation, Invitrogen NR3C1 antibody (Affinity BioReagents, MA1-510) was used in western blot on rat samples and in western blot on human samples (fig 1d). J Biol Chem (2012) ncbi
mouse monoclonal (BuGR2)
  • chromatin immunoprecipitation; mouse; 15 ug
In order to study the effect of DNA methylation on cell type-specific enhancer activity, Invitrogen NR3C1 antibody (ABR, MA1-510) was used in chromatin immunoprecipitation on mouse samples at 15 ug. EMBO J (2011) ncbi
Articles Reviewed
  1. Yasuda M, Shinoda M, Honda K, Fujita M, Kawata A, Nagashima H, et al. Maternal Separation Induces Orofacial Mechanical Allodynia in Adulthood. J Dent Res. 2016;95:1191-7 pubmed publisher
  2. Demuyser T, Bentea E, Deneyer L, Albertini G, Massie A, Smolders I. Disruption of the HPA-axis through corticosterone-release pellets induces robust depressive-like behavior and reduced BDNF levels in mice. Neurosci Lett. 2016;626:119-25 pubmed publisher
  3. Ma X, Xu L, Mueller E. Forkhead box A3 mediates glucocorticoid receptor function in adipose tissue. Proc Natl Acad Sci U S A. 2016;113:3377-82 pubmed publisher
  4. Baker G, Murphy T, Block T, Nguyen D, Lynch F. Development and validation of an immunohistochemistry assay to assess glucocorticoid receptor expression for clinical trials of mifepristone in breast cancer. Cancer Manag Res. 2015;7:361-8 pubmed publisher
  5. Arango Lievano M, Lambert W, Bath K, Garabedian M, Chao M, Jeanneteau F. Neurotrophic-priming of glucocorticoid receptor signaling is essential for neuronal plasticity to stress and antidepressant treatment. Proc Natl Acad Sci U S A. 2015;112:15737-42 pubmed publisher
  6. Stavreva D, Coulon A, Baek S, Sung M, John S, Stixova L, et al. Dynamics of chromatin accessibility and long-range interactions in response to glucocorticoid pulsing. Genome Res. 2015;25:845-57 pubmed publisher
  7. Shang H, Meng W, Sloboda D, Li S, Ehrlich L, Plagemann A, et al. Effects of maternal dexamethasone treatment early in pregnancy on glucocorticoid receptors in the ovine placenta. Reprod Sci. 2015;22:534-44 pubmed publisher
  8. Connors E, Shaik A, Migliore M, Kentner A. Environmental enrichment mitigates the sex-specific effects of gestational inflammation on social engagement and the hypothalamic pituitary adrenal axis-feedback system. Brain Behav Immun. 2014;42:178-90 pubmed publisher
  9. Lambert W, Xu C, Neubert T, Chao M, Garabedian M, Jeanneteau F. Brain-derived neurotrophic factor signaling rewrites the glucocorticoid transcriptome via glucocorticoid receptor phosphorylation. Mol Cell Biol. 2013;33:3700-14 pubmed publisher
  10. Blackford J, Guo C, Zhu R, Dougherty E, Chow C, Simons S. Identification of location and kinetically defined mechanism of cofactors and reporter genes in the cascade of steroid-regulated transactivation. J Biol Chem. 2012;287:40982-95 pubmed publisher
  11. Wiench M, John S, Baek S, Johnson T, Sung M, Escobar T, et al. DNA methylation status predicts cell type-specific enhancer activity. EMBO J. 2011;30:3028-39 pubmed publisher