This is a Validated Antibody Database (VAD) review about rat p35, based on 21 published articles (read how Labome selects the articles), using p35 antibody in all methods. It is aimed to help Labome visitors find the most suited p35 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
p35 synonym: Cdk5r; p35

Cell Signaling Technology
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:1000; loading ...; fig s4e
Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on mouse samples at 1:1000 (fig s4e). Nat Commun (2021) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:1000; loading ...; fig 2n
Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on mouse samples at 1:1000 (fig 2n). Int J Mol Sci (2021) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:250; loading ...; fig 2e
Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on mouse samples at 1:250 (fig 2e). Front Synaptic Neurosci (2020) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:1000; loading ...; fig 3a
Cell Signaling Technology p35 antibody (Cell Signaling, 2680S) was used in western blot on mouse samples at 1:1000 (fig 3a). Nature (2019) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; loading ...; fig 3b
Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on mouse samples (fig 3b). Nature (2019) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; human; 1:1000; loading ...; fig s1c
In order to study the function of liprin alpha 1 phosphorylation in synapse development, Cell Signaling Technology p35 antibody (Cell Signaling Technology, C64B10) was used in western blot on human samples at 1:1000 (fig s1c). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:500; fig 4
In order to elucidate modification microglial phenotype and neuroprotection in P301S tau transgenic mice via TREM2, Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on mouse samples at 1:500 (fig 4). Neuropharmacology (2016) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; rat; fig 2c
Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on rat samples (fig 2c). J Diabetes Res (2016) ncbi
domestic rabbit monoclonal (C64B10)
  • immunoprecipitation; rat; fig 4
  • western blot; rat; 1:1000; fig 3
  • immunoprecipitation; mouse; fig 2
  • western blot; mouse; 1:1000; fig 2
In order to report that p35 S-nitrosylation by nitric oxide signaling regulates hippocampal synaptic strength, Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in immunoprecipitation on rat samples (fig 4), in western blot on rat samples at 1:1000 (fig 3), in immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples at 1:1000 (fig 2). Nat Commun (2015) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; rat; 1:1000; fig 5c
In order to characterize the death target of Alzheimer patient amyloid-beta assembly, Na, K-ATPase alpha3, Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on rat samples at 1:1000 (fig 5c). Proc Natl Acad Sci U S A (2015) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; fig 4
In order to test the effects of high fat diet using a mouse model of familial Alzheimer disease, Cell Signaling Technology p35 antibody (Cell signaling, 2680) was used in western blot on mouse samples (fig 4). Biochim Biophys Acta (2015) ncbi
domestic rabbit monoclonal (C64B10)
  • immunohistochemistry; mouse; 1:500
In order to determine if tau phosphorylation contributes to spinal muscular atrophy pathogenesis, Cell Signaling Technology p35 antibody (Cell Signaling Technology, 2680) was used in immunohistochemistry on mouse samples at 1:500. J Neurosci (2015) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:1000; fig 10
In order to characterize cognitive and neuropathological Alzheimer's disease markers in a novel mouse model, Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on mouse samples at 1:1000 (fig 10). Age (Dordr) (2015) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:1000; fig 6
Cell Signaling Technology p35 antibody (Cell Signaling, 2680) was used in western blot on mouse samples at 1:1000 (fig 6). elife (2015) ncbi
domestic rabbit monoclonal (C64B10)
  • immunohistochemistry - frozen section; mouse; 1:500; fig s7c
In order to study the initiation and regulation of radial migration, Cell Signaling Technology p35 antibody (Cell Signaling Technology, 2680) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig s7c). Nat Commun (2014) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; 1:1000; fig 3
In order to study vascular endothelial growth factor signaling in mice infected with Plasmodium berghei ANKA, Cell Signaling Technology p35 antibody (Cell Signaling Technology, C64B10) was used in western blot on mouse samples at 1:1000 (fig 3). Front Immunol (2014) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; mouse; fig 7
In order to investigate the abnormalites in hippocampal energy metabolism in the pathogenesis of Alzheimer disease, Cell Signaling Technology p35 antibody (Cell signaling, 2680) was used in western blot on mouse samples (fig 7). Biochim Biophys Acta (2014) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; rat; 1:1000
Cell Signaling Technology p35 antibody (CST, 2680) was used in western blot on rat samples at 1:1000. Brain Res Bull (2014) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; human; 1:500; fig s3
In order to investigate the role of repressor element 1-silencing transcription factor in neurodegeneration during ageing, Cell Signaling Technology p35 antibody (Cell signaling, 2680) was used in western blot on human samples at 1:500 (fig s3). Nature (2014) ncbi
domestic rabbit monoclonal (C64B10)
  • western blot; rat; 1:1000
In order to study the role of calpain-regulated p35/cdk5 in the mechanism by which the PDE5 inhibitor sildenafil protects against malonate-induced chemical hypoxia, Cell Signaling Technology p35 antibody (Cell Signaling Technology, 2680) was used in western blot on rat samples at 1:1000. Biochim Biophys Acta (2013) ncbi
domestic rabbit monoclonal (C64B10)
  • immunohistochemistry - frozen section; rat; 1:100
Cell Signaling Technology p35 antibody (Cell Signaling, 2680S) was used in immunohistochemistry - frozen section on rat samples at 1:100. PLoS ONE (2012) ncbi
Articles Reviewed
  1. Hu D, Sun X, Magpusao A, Fedorov Y, Thompson M, Wang B, et al. Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. Nat Commun. 2021;12:5305 pubmed publisher
  2. López Gambero A, Rosell Valle C, Medina Vera D, Navarro J, Vargas A, Rivera P, et al. A Negative Energy Balance Is Associated with Metabolic Dysfunctions in the Hypothalamus of a Humanized Preclinical Model of Alzheimer's Disease, the 5XFAD Mouse. Int J Mol Sci. 2021;22: pubmed publisher
  3. Sahasrabudhe A, Begum F, Guevara C, Morrison C, Hsiao K, Kezunovic N, et al. Cyfip1 Regulates SynGAP1 at Hippocampal Synapses. Front Synaptic Neurosci. 2020;12:581714 pubmed publisher
  4. Ising C, Venegas C, Zhang S, Scheiblich H, Schmidt S, Vieira Saecker A, et al. NLRP3 inflammasome activation drives tau pathology. Nature. 2019;: pubmed publisher
  5. Faraco G, Hochrainer K, Segarra S, Schaeffer S, Santisteban M, Menon A, et al. Dietary salt promotes cognitive impairment through tau phosphorylation. Nature. 2019;: pubmed publisher
  6. Huang H, Lin X, Liang Z, Zhao T, Du S, Loy M, et al. Cdk5-dependent phosphorylation of liprin?1 mediates neuronal activity-dependent synapse development. Proc Natl Acad Sci U S A. 2017;114:E6992-E7001 pubmed publisher
  7. Jiang T, Zhang Y, Chen Q, Gao Q, Zhu X, Zhou J, et al. TREM2 modifies microglial phenotype and provides neuroprotection in P301S tau transgenic mice. Neuropharmacology. 2016;105:196-206 pubmed publisher
  8. Draney C, Hobson A, Grover S, Jack B, Tessem J. Cdk5r1 Overexpression Induces Primary β-Cell Proliferation. J Diabetes Res. 2016;2016:6375804 pubmed publisher
  9. Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed publisher
  10. Ohnishi T, Yanazawa M, Sasahara T, Kitamura Y, Hiroaki H, Fukazawa Y, et al. Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A. 2015;112:E4465-74 pubmed publisher
  11. Petrov D, Pedrós I, Artiach G, Sureda F, Barroso E, Pallas M, et al. High-fat diet-induced deregulation of hippocampal insulin signaling and mitochondrial homeostasis deficiences contribute to Alzheimer disease pathology in rodents. Biochim Biophys Acta. 2015;1852:1687-99 pubmed publisher
  12. Miller N, Feng Z, Edens B, Yang B, Shi H, Sze C, et al. Non-aggregating tau phosphorylation by cyclin-dependent kinase 5 contributes to motor neuron degeneration in spinal muscular atrophy. J Neurosci. 2015;35:6038-50 pubmed publisher
  13. Porquet D, Andrés Benito P, Griñán Ferré C, Camins A, Ferrer I, Canudas A, et al. Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8). Age (Dordr). 2015;37:9747 pubmed publisher
  14. Altmeppen H, Prox J, Krasemann S, Puig B, Kruszewski K, Dohler F, et al. The sheddase ADAM10 is a potent modulator of prion disease. elife. 2015;4: pubmed publisher
  15. Ye T, Ip J, Fu A, Ip N. Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex. Nat Commun. 2014;5:4826 pubmed publisher
  16. Hempel C, Hoyer N, Kildemoes A, Jendresen C, Kurtzhals J. Systemic and Cerebral Vascular Endothelial Growth Factor Levels Increase in Murine Cerebral Malaria along with Increased Calpain and Caspase Activity and Can be Reduced by Erythropoietin Treatment. Front Immunol. 2014;5:291 pubmed publisher
  17. Pedr s I, Petrov D, Allgaier M, Sureda F, Barroso E, Beas Zarate C, et al. Early alterations in energy metabolism in the hippocampus of APPswe/PS1dE9 mouse model of Alzheimer's disease. Biochim Biophys Acta. 2014;1842:1556-66 pubmed publisher
  18. Liu X, Liu Y, Zhang J, Zhang W, Sun Y, Gu X, et al. Intrathecal administration of roscovitine prevents remifentanil-induced postoperative hyperalgesia and decreases the phosphorylation of N-methyl-D-aspartate receptor and metabotropic glutamate receptor 5 in spinal cord. Brain Res Bull. 2014;106:9-16 pubmed publisher
  19. Lu T, Aron L, Zullo J, Pan Y, Kim H, Chen Y, et al. REST and stress resistance in ageing and Alzheimer's disease. Nature. 2014;507:448-54 pubmed publisher
  20. Barros Miñones L, Martín de Saavedra D, Perez Alvarez S, Orejana L, Suquía V, Goni Allo B, et al. Inhibition of calpain-regulated p35/cdk5 plays a central role in sildenafil-induced protection against chemical hypoxia produced by malonate. Biochim Biophys Acta. 2013;1832:705-17 pubmed publisher
  21. Zhang H, Zhang X, Wang W, Xue Q, Lu H, Huang J, et al. Increased synaptophysin is involved in inflammation-induced heat hyperalgesia mediated by cyclin-dependent kinase 5 in rats. PLoS ONE. 2012;7:e46666 pubmed publisher