This is a Validated Antibody Database (VAD) review about rat Xrcc5, based on 7 published articles (read how Labome selects the articles), using Xrcc5 antibody in all methods. It is aimed to help Labome visitors find the most suited Xrcc5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Xrcc5 synonym: Ku80; Kup80; X-ray repair cross-complementing protein 5; Ku autoantigen, 80kD); X-ray repair complementing defective repair in Chinese hamster cells 5; X-ray repair cross complementation (double-strand-break rejoining; X-ray repair cross complementation (double-strand-break rejoining; Ku autoantigen, 80kD)

Invitrogen
mouse monoclonal (162)
  • chromatin immunoprecipitation; mouse; loading ...; fig 5a
  • EMSA; mouse; loading ...; fig 5f
In order to assess the contributions of single-strand annealing factors HR Rad52 and translesion DNA polymerase q to CSR, Invitrogen Xrcc5 antibody (Thermo Fisher Scientific, MA1-21818) was used in chromatin immunoprecipitation on mouse samples (fig 5a) and in EMSA on mouse samples (fig 5f). Nat Commun (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 3
In order to study how miR-622 promotes resistance to PARP inhibitors and platinum in BRCA1 mutant high-grade serous ovarian carcinomas, Invitrogen Xrcc5 antibody (Thermo Scientific, PA5-17454) was used in western blot on human samples (fig 3). Cell Rep (2016) ncbi
mouse monoclonal (162)
  • immunohistochemistry - paraffin section; human; 1:200; fig 6
  • immunocytochemistry; human; 1:200; fig 5
  • western blot; human; 1:1000; fig 5
In order to analyze chemotherapeutic agent treatment and the DNA damage/repair cascade in glioblastoma cell lines, Invitrogen Xrcc5 antibody (NeoMarkers, MS-286) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 6), in immunocytochemistry on human samples at 1:200 (fig 5) and in western blot on human samples at 1:1000 (fig 5). Int J Oncol (2015) ncbi
mouse monoclonal (162)
  • immunoprecipitation; human; 10 ug
In order to investigate the role of TRF2/RAP1 and DNA-PK at telomeric fusion, Invitrogen Xrcc5 antibody (Thermo Scientific, Ab3-162) was used in immunoprecipitation on human samples at 10 ug. EMBO J (2010) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig s1a
Cell Signaling Technology Xrcc5 antibody (Cell Signaling, 2753) was used in western blot on human samples (fig s1a). Cell Death Dis (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; fig 4
  • western blot; mouse; 1:3000; fig 4
Cell Signaling Technology Xrcc5 antibody (Cell Signaling Technology, 2753S) was used in immunoprecipitation on mouse samples (fig 4) and in western blot on mouse samples at 1:3000 (fig 4). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 7
Cell Signaling Technology Xrcc5 antibody (Cell Signaling, 2753) was used in western blot on mouse samples at 1:1000 (fig 7). Stem Cell Reports (2015) ncbi
Articles Reviewed
  1. Zan H, Tat C, Qiu Z, Taylor J, Guerrero J, Shen T, et al. Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination. Nat Commun. 2017;8:14244 pubmed publisher
  2. Narayanaswamy P, Tkachuk S, Haller H, Dumler I, Kiyan Y. CHK1 and RAD51 activation after DNA damage is regulated via urokinase receptor/TLR4 signaling. Cell Death Dis. 2016;7:e2383 pubmed publisher
  3. Choi Y, Meghani K, Brault M, Leclerc L, He Y, Day T, et al. Platinum and PARP Inhibitor Resistance Due to Overexpression of MicroRNA-622 in BRCA1-Mutant Ovarian Cancer. Cell Rep. 2016;14:429-439 pubmed publisher
  4. Liu X, Chandramouly G, Rass E, Guan Y, Wang G, Hobbs R, et al. LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair. Nat Commun. 2015;6:8325 pubmed publisher
  5. Chang C, Zhang M, Rajapakshe K, Coarfa C, Edwards D, Huang S, et al. Mammary Stem Cells and Tumor-Initiating Cells Are More Resistant to Apoptosis and Exhibit Increased DNA Repair Activity in Response to DNA Damage. Stem Cell Reports. 2015;5:378-91 pubmed publisher
  6. Annovazzi L, Caldera V, Mellai M, Riganti C, Battaglia L, Chirio D, et al. The DNA damage/repair cascade in glioblastoma cell lines after chemotherapeutic agent treatment. Int J Oncol. 2015;46:2299-308 pubmed publisher
  7. Bombarde O, Boby C, Gomez D, Frit P, Giraud Panis M, Gilson E, et al. TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends. EMBO J. 2010;29:1573-84 pubmed publisher