This is a Validated Antibody Database (VAD) review about rat Wt1, based on 13 published articles (read how Labome selects the articles), using Wt1 antibody in all methods. It is aimed to help Labome visitors find the most suited Wt1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Santa Cruz Biotechnology
mouse monoclonal (F-6)
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig 1a
  • immunocytochemistry; mouse; 1:100; fig 1e
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, SC-7385) was used in immunohistochemistry - frozen section on mouse samples at 1:400 (fig 1a) and in immunocytochemistry on mouse samples at 1:100 (fig 1e). Stem Cell Res Ther (2022) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry; mouse; loading ...; fig 1i
  • immunohistochemistry; human; fig 2a
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz Biotechnology, sc-7385) was used in immunohistochemistry on mouse samples (fig 1i) and in immunohistochemistry on human samples (fig 2a). Cell Death Dis (2021) ncbi
mouse monoclonal (H-1)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2c
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz Biotechnology, sc-393498) was used in immunohistochemistry on mouse samples at 1:100 (fig 2c). Cells (2021) ncbi
mouse monoclonal (H-1)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 5g
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz Biotechnology, sc-393498) was used in immunohistochemistry on mouse samples at 1:1000 (fig 5g). Cell Death Dis (2021) ncbi
mouse monoclonal (F-6)
  • western blot; mouse; 1:750; loading ...; fig 3e
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc7385) was used in western blot on mouse samples at 1:750 (fig 3e). EMBO Mol Med (2019) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry - paraffin section; rat; loading ...; fig s6
Santa Cruz Biotechnology Wt1 antibody (SantaCruz, sc-7385) was used in immunohistochemistry - paraffin section on rat samples (fig s6). J Clin Invest (2017) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry; mouse; loading ...; fig 2a
In order to perform whole exome sequencing to identify recessive causes of steroid-resistant nephrotic syndrome, Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc-7385) was used in immunohistochemistry on mouse samples (fig 2a). J Clin Invest (2017) ncbi
mouse monoclonal (F-6)
  • chromatin immunoprecipitation; human; loading ...; fig 3a
  • western blot; human; loading ...; fig 1d
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz Biotechnology, F-6) was used in chromatin immunoprecipitation on human samples (fig 3a) and in western blot on human samples (fig 1d). Biochem Biophys Res Commun (2017) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry - paraffin section; mouse; fig 2
  • western blot; mouse; fig 1
In order to analyze infertility in mice by deletion of the tyrosine phosphatase Shp2 in Sertoli cells, Santa Cruz Biotechnology Wt1 antibody (Santa Cruz Biotechnology, sc-7385) was used in immunohistochemistry - paraffin section on mouse samples (fig 2) and in western blot on mouse samples (fig 1). Sci Rep (2015) ncbi
mouse monoclonal (F-6)
  • western blot; mouse; loading ...; fig 4g
In order to develop and characterize a mouse model of glomerular disease, Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc-7385) was used in western blot on mouse samples (fig 4g). J Clin Invest (2015) ncbi
mouse monoclonal (F-6)
  • immunoprecipitation; human; fig 2b
  • immunocytochemistry; human; fig 2c
  • western blot; human; 1:1000; fig 6e
  • immunoprecipitation; mouse; fig 2d
  • western blot; mouse; 1:1000; fig 6e
Santa Cruz Biotechnology Wt1 antibody (Santa, sc-7385) was used in immunoprecipitation on human samples (fig 2b), in immunocytochemistry on human samples (fig 2c), in western blot on human samples at 1:1000 (fig 6e), in immunoprecipitation on mouse samples (fig 2d) and in western blot on mouse samples at 1:1000 (fig 6e). Nat Commun (2014) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry; mouse; 1:1000; fig 6
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc-7385) was used in immunohistochemistry on mouse samples at 1:1000 (fig 6). Stem Cells (2010) ncbi
Abcam
domestic rabbit monoclonal (EPR23963-116)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 3b
Abcam Wt1 antibody (Abcam, ab267377) was used in immunohistochemistry on mouse samples at 1:200 (fig 3b). Int J Mol Med (2021) ncbi
Articles Reviewed
  1. Zhu H, Liu X, DING Y, Tan K, Ni W, Ouyang W, et al. IL-6 coaxes cellular dedifferentiation as a pro-regenerative intermediate that contributes to pericardial ADSC-induced cardiac repair. Stem Cell Res Ther. 2022;13:44 pubmed publisher
  2. Lü Z, Liu H, Song N, Liang Y, Zhu J, Chen J, et al. METTL14 aggravates podocyte injury and glomerulopathy progression through N6-methyladenosine-dependent downregulating of Sirt1. Cell Death Dis. 2021;12:881 pubmed publisher
  3. Mangold N, Pippin J, Unnersjoe Jess D, Koehler S, Shankland S, Brähler S, et al. The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development. Cells. 2021;10: pubmed publisher
  4. Zhao J, Chen J, Li Y, Xia L, Wu Y. Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021;48: pubmed publisher
  5. Nishad R, Mukhi D, Singh A, Motrapu M, Chintala K, Tammineni P, et al. Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria. Cell Death Dis. 2021;12:342 pubmed publisher
  6. Lin C, Hsu Y, Huang Y, Shih Y, Wang C, Chiang W, et al. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction. EMBO Mol Med. 2019;11: pubmed publisher
  7. Rao J, Ashraf S, Tan W, van der Ven A, Gee H, Braun D, et al. Advillin acts upstream of phospholipase C ϵ1 in steroid-resistant nephrotic syndrome. J Clin Invest. 2017;127:4257-4269 pubmed publisher
  8. Lovric S, Gonçalves S, Gee H, Oskouian B, Srinivas H, Choi W, et al. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency. J Clin Invest. 2017;127:912-928 pubmed publisher
  9. Ullmark T, Montano G, Järvstråt L, Jernmark Nilsson H, Håkansson E, Drott K, et al. Anti-apoptotic quinolinate phosphoribosyltransferase (QPRT) is a target gene of Wilms' tumor gene 1 (WT1) protein in leukemic cells. Biochem Biophys Res Commun. 2017;482:802-807 pubmed publisher
  10. Hu X, Tang Z, Li Y, Liu W, Zhang S, Wang B, et al. Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice. Sci Rep. 2015;5:12982 pubmed publisher
  11. Wang L, Jirka G, Rosenberg P, Buckley A, Gomez J, Fields T, et al. Gq signaling causes glomerular injury by activating TRPC6. J Clin Invest. 2015;125:1913-26 pubmed publisher
  12. Shandilya J, Toska E, Richard D, Medler K, Roberts S. WT1 interacts with MAD2 and regulates mitotic checkpoint function. Nat Commun. 2014;5:4903 pubmed publisher
  13. Simon L, Ekman G, Garcia T, Carnes K, Zhang Z, Murphy T, et al. ETV5 regulates sertoli cell chemokines involved in mouse stem/progenitor spermatogonia maintenance. Stem Cells. 2010;28:1882-92 pubmed publisher