This is a Validated Antibody Database (VAD) review about rat Vcp, based on 40 published articles (read how Labome selects the articles), using Vcp antibody in all methods. It is aimed to help Labome visitors find the most suited Vcp antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Vcp synonym: transitional endoplasmic reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit; TER ATPase

Abcam
mouse monoclonal (5)
  • western blot; mouse; 1:2000; loading ...; fig 6c
Abcam Vcp antibody (Abcam, ab11433) was used in western blot on mouse samples at 1:2000 (fig 6c). EMBO Mol Med (2018) ncbi
mouse monoclonal (5)
  • immunoprecipitation; human; loading ...; fig 7a
Abcam Vcp antibody (Abcam, ab11433) was used in immunoprecipitation on human samples (fig 7a). EMBO J (2018) ncbi
mouse monoclonal (5)
  • immunoprecipitation; human; loading ...; fig s2a
Abcam Vcp antibody (Abcam, ab11433) was used in immunoprecipitation on human samples (fig s2a). Proc Natl Acad Sci U S A (2017) ncbi
rabbit monoclonal (EPR3307(2))
  • western blot; mouse; 1:10,000; fig 4b
  • western blot; human; 1:10,000; loading ...; fig 3a
Abcam Vcp antibody (Abcam, ab109240) was used in western blot on mouse samples at 1:10,000 (fig 4b) and in western blot on human samples at 1:10,000 (fig 3a). Biochim Biophys Acta Mol Basis Dis (2017) ncbi
rabbit monoclonal (EPR3307(2))
  • western blot; mouse; 1:10,000; fig 4a
Abcam Vcp antibody (Abcam, ab109240) was used in western blot on mouse samples at 1:10,000 (fig 4a). Nat Commun (2016) ncbi
rabbit monoclonal (EPR3307(2))
  • western blot; human; fig 4
Abcam Vcp antibody (Epitomics, 3339-1) was used in western blot on human samples (fig 4). J Biol Chem (2016) ncbi
mouse monoclonal (5)
  • western blot; human; 1:1000; fig 6
Abcam Vcp antibody (Abcam, Ab11433) was used in western blot on human samples at 1:1000 (fig 6). Nat Commun (2016) ncbi
rabbit monoclonal (EPR3307(2))
  • immunocytochemistry; human; 1:1000; fig 3
  • western blot; human; fig s2
Abcam Vcp antibody (Abcam, ab109240) was used in immunocytochemistry on human samples at 1:1000 (fig 3) and in western blot on human samples (fig s2). Cell Cycle (2016) ncbi
mouse monoclonal (5)
  • immunohistochemistry - paraffin section; mouse; 1:250; fig 3
  • western blot; mouse; 1:2000; fig 2
  • western blot; human; 1:2000; fig 4
Abcam Vcp antibody (Abcam, ab11433) was used in immunohistochemistry - paraffin section on mouse samples at 1:250 (fig 3), in western blot on mouse samples at 1:2000 (fig 2) and in western blot on human samples at 1:2000 (fig 4). PLoS ONE (2015) ncbi
mouse monoclonal (5)
  • immunohistochemistry; human; loading ...; fig 3c
Abcam Vcp antibody (Abcam, ab11433) was used in immunohistochemistry on human samples (fig 3c). J Biol Chem (2016) ncbi
rabbit monoclonal (EPR3307(2))
  • immunohistochemistry; human; loading ...; fig 3c
Abcam Vcp antibody (Abcam, ab109240) was used in immunohistochemistry on human samples (fig 3c). J Biol Chem (2016) ncbi
rabbit monoclonal (EPR3307(2))
  • western blot; human; fig 6
Abcam Vcp antibody (Abcam, ab109240) was used in western blot on human samples (fig 6). Oncotarget (2015) ncbi
mouse monoclonal (5)
  • western blot; mouse; fig s3
Abcam Vcp antibody (Abcam, ab11433) was used in western blot on mouse samples (fig s3). Mol Metab (2015) ncbi
mouse monoclonal (5)
  • immunohistochemistry - paraffin section; dog; 1:4000; fig 1
  • western blot; dog; fig 1
Abcam Vcp antibody (Abcam, ab11433) was used in immunohistochemistry - paraffin section on dog samples at 1:4000 (fig 1) and in western blot on dog samples (fig 1). BMC Cancer (2015) ncbi
mouse monoclonal (5)
  • western blot; mouse; fig 3
Abcam Vcp antibody (Abcam, ab11433) was used in western blot on mouse samples (fig 3). EMBO Mol Med (2015) ncbi
mouse monoclonal (5)
  • western blot; mouse
Abcam Vcp antibody (Abcam, ab11433) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; loading ...; fig s2e
In order to understand the interaction between PTEN and FBXL2 in the context of apoptosis, Invitrogen Vcp antibody (Thermo Scientific, PA5-22257) was used in western blot on human samples (fig s2e). Nature (2017) ncbi
mouse monoclonal (5)
  • immunocytochemistry; human; loading ...; fig 4a
In order to characterize the components of stress granules, Invitrogen Vcp antibody (Thermo Fisher Scientific, MA3-004) was used in immunocytochemistry on human samples (fig 4a). EMBO J (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:200; loading ...; fig 4f
In order to find host factors important for EV71 replication in human rhabdomyosarcoma cells, Invitrogen Vcp antibody (Pierce, PA5-22257) was used in immunohistochemistry on human samples at 1:200 (fig 4f). Nat Commun (2016) ncbi
mouse monoclonal (5)
  • western blot; human; fig 2
In order to demonstrate that DNA-damage inducible 1 homolog 2 is required to cleave and activate Nrf1, Invitrogen Vcp antibody (Invitrogen, MA3-004) was used in western blot on human samples (fig 2). elife (2016) ncbi
mouse monoclonal (5)
  • western blot; human; fig 1b
In order to assess inhibition of the SUMOylation of VCP that leads to impaired stress response by pathogenic mutations in the valosin-containing protein/p97(VCP) N-domain, Invitrogen Vcp antibody (Pierce, MA3-004) was used in western blot on human samples (fig 1b). J Biol Chem (2016) ncbi
mouse monoclonal (5)
  • immunocytochemistry; mouse; 1:2000; fig s2
  • western blot; mouse; 1:5000; fig 1
In order to research delayed stress granule resolution on oxidative stress from myoblast C2C12 transfected with mutant valosin-containing protein, Invitrogen Vcp antibody (Pierce, MA3-004) was used in immunocytochemistry on mouse samples at 1:2000 (fig s2) and in western blot on mouse samples at 1:5000 (fig 1). Am J Pathol (2016) ncbi
mouse monoclonal (5)
  • immunohistochemistry - paraffin section; human; 1:5000; loading ...; tbl 1
In order to examine eosinophilic neuronal cytoplasmic inclusions, Invitrogen Vcp antibody (Thermo SCIENTIFIC, 5) was used in immunohistochemistry - paraffin section on human samples at 1:5000 (tbl 1). Neuropathology (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:10,000; fig e 1
In order to find out the reason ASS1-deficient tumors undergo de novo pyrimidine synthesis without aspartate, Invitrogen Vcp antibody (Thermo Scientific, PA5-22257) was used in western blot on human samples at 1:10,000 (fig e 1). Nature (2015) ncbi
mouse monoclonal (5)
  • immunohistochemistry - paraffin section; human; 1:5,000
In order to investigate the association of eosinophilic neuronal cytoplasmic inclusions with stress granules and autophagy in two clinical cases, Invitrogen Vcp antibody (Thermo SCIENTIFIC, 5) was used in immunohistochemistry - paraffin section on human samples at 1:5,000. Neuropathology (2014) ncbi
mouse monoclonal (5)
  • immunohistochemistry; mouse; fig 6
In order to determine ER quality control compartments created by amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions, Invitrogen Vcp antibody (Thermo Scientific, Ma3-004) was used in immunohistochemistry on mouse samples (fig 6). Acta Neuropathol Commun (2013) ncbi
mouse monoclonal (5)
  • immunoprecipitation; human
  • immunohistochemistry; human; 1:2000
In order to study the effect of valosin-containing protein mutations on ubiquitin and TDP-43 accumulation and cognitive function in mice, Invitrogen Vcp antibody (Pierce Biotechnology, MA3-004) was used in immunoprecipitation on human samples and in immunohistochemistry on human samples at 1:2000. Am J Pathol (2013) ncbi
Novus Biologicals
mouse monoclonal (5)
  • immunocytochemistry; African green monkey; loading ...; fig 6f
In order to elucidate how cells handle and respond to misfolded Caveolin-1, Novus Biologicals Vcp antibody (Novus, NB120-11433) was used in immunocytochemistry on African green monkey samples (fig 6f). Sci Rep (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 3f
Cell Signaling Technology Vcp antibody (Cell Signaling Technology, 2648) was used in western blot on human samples (fig 3f). J Clin Invest (2018) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s2b-c
In order to discuss how histone deacetylase 4 mutations contribute to humans with eating disorders, Cell Signaling Technology Vcp antibody (Cell signaling, 2648) was used in western blot on mouse samples at 1:1000 (fig s2b-c). Biol Psychiatry (2017) ncbi
rabbit monoclonal (7F3)
  • western blot; mouse; 1:1000; fig s4
  • western blot; human; 1:1000; fig s4
Cell Signaling Technology Vcp antibody (Cell Signaling, 2649S) was used in western blot on mouse samples at 1:1000 (fig s4) and in western blot on human samples at 1:1000 (fig s4). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology Vcp antibody (Cell Signaling, 2648S) was used in western blot on human samples . PLoS ONE (2014) ncbi
BD Biosciences
mouse monoclonal (18/VCP)
  • immunohistochemistry; human; loading ...; fig 1s4d
BD Biosciences Vcp antibody (BD Biosciences, 612183) was used in immunohistochemistry on human samples (fig 1s4d). elife (2019) ncbi
mouse monoclonal (18/VCP)
  • western blot; human; loading ...; fig 4a
BD Biosciences Vcp antibody (BD Transduction Laboratories, 612182) was used in western blot on human samples (fig 4a). Sci Rep (2017) ncbi
mouse monoclonal (18/VCP)
  • chromatin immunoprecipitation; common platanna; fig 4e
In order to elucidate how the CMG complex is displaced from chromatin, BD Biosciences Vcp antibody (BD Biosciences, 612182) was used in chromatin immunoprecipitation on common platanna samples (fig 4e). Mol Cell Biol (2016) ncbi
mouse monoclonal (18/VCP)
  • western blot; mouse; 1:1000; fig 3
BD Biosciences Vcp antibody (BD Bioscience, 612183) was used in western blot on mouse samples at 1:1000 (fig 3). Sci Rep (2016) ncbi
mouse monoclonal (18/VCP)
  • immunocytochemistry; mouse; fig 6
  • western blot; mouse; 1:1000; fig 7a
In order to show how ATL1 and VCP work together to regulate endoplasmic reticulum and protein synthesis for dendritic spine formation, BD Biosciences Vcp antibody (BD Biosciences, 612182) was used in immunocytochemistry on mouse samples (fig 6) and in western blot on mouse samples at 1:1000 (fig 7a). Nat Commun (2016) ncbi
mouse monoclonal (18/VCP)
  • western blot; human; 1:1000
In order to examine palmitoyl protein thioesterase 1, BD Biosciences Vcp antibody (BD Transduction LaboratoriesTM, 612182) was used in western blot on human samples at 1:1000. Data Brief (2015) ncbi
mouse monoclonal (18/VCP)
  • western blot; human; 1:1000
In order to identify substrates of human PPT1 using neuronal cells, BD Biosciences Vcp antibody (BD Transduction Laboratories™, 612182) was used in western blot on human samples at 1:1000. J Proteomics (2015) ncbi
mouse monoclonal (18/VCP)
  • flow cytometry; human; 1:1000
  • immunoprecipitation; human; 1:1000
  • western blot; human; 1:1000
BD Biosciences Vcp antibody (BD Transduction Laboratories, 612183) was used in flow cytometry on human samples at 1:1000, in immunoprecipitation on human samples at 1:1000 and in western blot on human samples at 1:1000. Nat Commun (2014) ncbi
mouse monoclonal (18/VCP)
  • western blot; human
In order to investigate the role of Epstein-Barr virus large tegument protein BPLF1 in innate immune evasion and its mechanism, BD Biosciences Vcp antibody (BD Transduction laboratories, 612183) was used in western blot on human samples . PLoS Pathog (2014) ncbi
Articles Reviewed
  1. Zhang P, Fan B, Yang P, Temirov J, Messing J, Kim H, et al. Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. elife. 2019;8: pubmed publisher
  2. Bayindir Buchhalter I, Wolff G, Lerch S, Sijmonsma T, Schuster M, Gronych J, et al. Cited4 is a sex-biased mediator of the antidiabetic glitazone response in adipocyte progenitors. EMBO Mol Med. 2018;10: pubmed publisher
  3. Weniger M, Tiacci E, Schneider S, Arnolds J, Rüschenbaum S, Duppach J, et al. Human CD30+ B cells represent a unique subset related to Hodgkin lymphoma cells. J Clin Invest. 2018;128:2996-3007 pubmed publisher
  4. Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
  5. Kuchay S, Giorgi C, Simoneschi D, Pagan J, Missiroli S, Saraf A, et al. PTEN counteracts FBXL2 to promote IP3R3- and Ca2+-mediated apoptosis limiting tumour growth. Nature. 2017;546:554-558 pubmed publisher
  6. Matějů D, Franzmann T, Patel A, Kopach A, Boczek E, Maharana S, et al. An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function. EMBO J. 2017;36:1669-1687 pubmed publisher
  7. Nguyen T, Li J, Lu C, Mamrosh J, Lu G, Cathers B, et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc Natl Acad Sci U S A. 2017;114:3565-3571 pubmed publisher
  8. Erzurumlu Y, Ballar P. Androgen Mediated Regulation of Endoplasmic Reticulum-Associated Degradation and its Effects on Prostate Cancer. Sci Rep. 2017;7:40719 pubmed publisher
  9. Tiwari A, Copeland C, Han B, Hanson C, Raghunathan K, Kenworthy A. Caveolin-1 is an aggresome-inducing protein. Sci Rep. 2016;6:38681 pubmed publisher
  10. Guo X, Qi X. VCP cooperates with UBXD1 to degrade mitochondrial outer membrane protein MCL1 in model of Huntington's disease. Biochim Biophys Acta Mol Basis Dis. 2017;1863:552-559 pubmed publisher
  11. Lutter M, Khan M, Satio K, Davis K, Kidder I, McDaniel L, et al. The Eating-Disorder Associated HDAC4A778T Mutation Alters Feeding Behaviors in Female Mice. Biol Psychiatry. 2017;81:770-777 pubmed publisher
  12. Wu K, Phuektes P, Kumar P, Goh G, Moreau D, Chow V, et al. Human genome-wide RNAi screen reveals host factors required for enterovirus 71 replication. Nat Commun. 2016;7:13150 pubmed publisher
  13. Fullbright G, Rycenga H, Gruber J, Long D. p97 Promotes a Conserved Mechanism of Helicase Unloading during DNA Cross-Link Repair. Mol Cell Biol. 2016;36:2983-2994 pubmed publisher
  14. Hu H, Umemori H, Hsueh Y. Postsynaptic SDC2 induces transsynaptic signaling via FGF22 for bidirectional synaptic formation. Sci Rep. 2016;6:33592 pubmed publisher
  15. Guo X, Sun X, Hu D, Wang Y, Fujioka H, Vyas R, et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun. 2016;7:12646 pubmed publisher
  16. Koizumi S, Irie T, Hirayama S, Sakurai Y, Yashiroda H, Naguro I, et al. The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction. elife. 2016;5: pubmed publisher
  17. Wang T, Xu W, Qin M, Yang Y, Bao P, Shen F, et al. Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response. J Biol Chem. 2016;291:14373-84 pubmed publisher
  18. Rodriguez Ortiz C, Flores J, Valenzuela J, Rodriguez G, Zumkehr J, Tran D, et al. The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress. Am J Pathol. 2016;186:1623-34 pubmed publisher
  19. Shih Y, Hsueh Y. VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation. Nat Commun. 2016;7:11020 pubmed publisher
  20. Di X, Wang Y, Han D, Fu Y, Duerfeldt A, Blagg B, et al. Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation. J Biol Chem. 2016;291:9526-39 pubmed publisher
  21. Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed publisher
  22. Nabhan J, Wood K, Rao V, Morin J, Bhamidipaty S, LaBranche T, et al. Intrathecal delivery of frataxin mRNA encapsulated in lipid nanoparticles to dorsal root ganglia as a potential therapeutic for Friedreich's ataxia. Sci Rep. 2016;6:20019 pubmed publisher
  23. Franz A, Pirson P, Pilger D, Halder S, Achuthankutty D, Kashkar H, et al. Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression. Nat Commun. 2016;7:10612 pubmed publisher
  24. Kuo C, Li X, Stark J, Shih H, Ann D. RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner. Cell Cycle. 2016;15:787-98 pubmed publisher
  25. Rabinovich S, Adler L, Yizhak K, Sarver A, Silberman A, Agron S, et al. Diversion of aspartate in ASS1-deficient tumours fosters de novo pyrimidine synthesis. Nature. 2015;527:379-383 pubmed publisher
  26. Fusser M, Kernstock S, Aileni V, Egge Jacobsen W, Falnes P, Klungland A. Lysine Methylation of the Valosin-Containing Protein (VCP) Is Dispensable for Development and Survival of Mice. PLoS ONE. 2015;10:e0141472 pubmed publisher
  27. Berry G, Asokan A. Chemical Modulation of Endocytic Sorting Augments Adeno-associated Viral Transduction. J Biol Chem. 2016;291:939-47 pubmed publisher
  28. Zhou X, Wei J, Chen F, Xiao X, Huang T, He Q, et al. Epigenetic downregulation of the ISG15-conjugating enzyme UbcH8 impairs lipolysis and correlates with poor prognosis in nasopharyngeal carcinoma. Oncotarget. 2015;6:41077-91 pubmed publisher
  29. Okun J, Conway S, Schmidt K, Schumacher J, Wang X, de Guia R, et al. Molecular regulation of urea cycle function by the liver glucocorticoid receptor. Mol Metab. 2015;4:732-40 pubmed publisher
  30. Scifo E, Szwajda A, Soliymani R, Pezzini F, Bianchi M, Dapkunas A, et al. Quantitative analysis of PPT1 interactome in human neuroblastoma cells. Data Brief. 2015;4:207-16 pubmed publisher
  31. Nadeau M, Rico C, Tsoi M, Vivancos M, Filimon S, Paquet M, et al. Pharmacological targeting of valosin containing protein (VCP) induces DNA damage and selectively kills canine lymphoma cells. BMC Cancer. 2015;15:479 pubmed publisher
  32. Stoy C, Sundaram A, Rios Garcia M, Wang X, Seibert O, Zota A, et al. Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy. EMBO Mol Med. 2015;7:1048-62 pubmed publisher
  33. Scifo E, Szwajda A, Soliymani R, Pezzini F, Bianchi M, Dapkunas A, et al. Proteomic analysis of the palmitoyl protein thioesterase 1 interactome in SH-SY5Y human neuroblastoma cells. J Proteomics. 2015;123:42-53 pubmed publisher
  34. Mössenböck K, Vegiopoulos A, Rose A, Sijmonsma T, Herzig S, Schafmeier T. Browning of white adipose tissue uncouples glucose uptake from insulin signaling. PLoS ONE. 2014;9:e110428 pubmed publisher
  35. Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  36. van de Weijer M, Bassik M, Luteijn R, Voorburg C, Lohuis M, Kremmer E, et al. A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation. Nat Commun. 2014;5:3832 pubmed publisher
  37. van Gent M, Braem S, de Jong A, Delagic N, Peeters J, Boer I, et al. Epstein-Barr virus large tegument protein BPLF1 contributes to innate immune evasion through interference with toll-like receptor signaling. PLoS Pathog. 2014;10:e1003960 pubmed publisher
  38. Chen Q, Xu R, Zeng C, Lu Q, Huang D, Shi C, et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ?4-mediated FAK signaling. PLoS ONE. 2014;9:e88386 pubmed publisher
  39. Kuijpers M, van Dis V, Haasdijk E, Harterink M, Vocking K, Post J, et al. Amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions represent an ER quality control compartment. Acta Neuropathol Commun. 2013;1:24 pubmed publisher
  40. Rodriguez Ortiz C, Hoshino H, Cheng D, Liu Yescevitz L, Blurton Jones M, Wolozin B, et al. Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice. Am J Pathol. 2013;183:504-15 pubmed publisher