This is a Validated Antibody Database (VAD) review about rat Vamp2, based on 15 published articles (read how Labome selects the articles), using Vamp2 antibody in all methods. It is aimed to help Labome visitors find the most suited Vamp2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Vamp2 synonym: RATVAMPB; RATVAMPIR; SYB; Syb2

Synaptic Systems
mouse monoclonal (69.1)
  • western blot; human; 1:1000; loading ...; fig 3b
Synaptic Systems Vamp2 antibody (SySy, 104 211C5) was used in western blot on human samples at 1:1000 (fig 3b). elife (2020) ncbi
mouse monoclonal (69.1)
  • immunocytochemistry; bovine; fig 1b
  • western blot; bovine; fig s1
Synaptic Systems Vamp2 antibody (Synaptic Systems, 69.1) was used in immunocytochemistry on bovine samples (fig 1b) and in western blot on bovine samples (fig s1). Sci Rep (2017) ncbi
mouse monoclonal (69.1)
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 7e
Synaptic Systems Vamp2 antibody (SySy, 69.1) was used in immunocytochemistry on mouse samples at 1:1000 (fig 7e). J Neurosci (2017) ncbi
mouse monoclonal (69.1)
  • western blot; mouse; loading ...; fig 3a
In order to investigate the function of extended synaptotagmins in knock out mice, Synaptic Systems Vamp2 antibody (Synaptic systems, 69.1) was used in western blot on mouse samples (fig 3a). PLoS ONE (2016) ncbi
mouse monoclonal (69.1)
  • western blot; mouse; fig 6
  • western blot; human; fig 8
Synaptic Systems Vamp2 antibody (Synaptic Systems, cl. 69.1) was used in western blot on mouse samples (fig 6) and in western blot on human samples (fig 8). PLoS Biol (2015) ncbi
mouse monoclonal (69.1)
  • western blot; mouse; fig 6
In order to test if presenilin and nicastrin regulate synaptic function in a gamma-secretase-dependent or -independent manner, Synaptic Systems Vamp2 antibody (SYSY, 69.1) was used in western blot on mouse samples (fig 6). Proc Natl Acad Sci U S A (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR12790)
  • western blot; mouse; 1:1000; loading ...; fig 4g
Abcam Vamp2 antibody (Abcam, ab181869) was used in western blot on mouse samples at 1:1000 (fig 4g). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; rat; loading ...
Abcam Vamp2 antibody (Abcam, ab3347) was used in immunocytochemistry on rat samples . Sci Adv (2021) ncbi
domestic rabbit monoclonal (EPR12790)
  • western blot; mouse; fig 8
Abcam Vamp2 antibody (Abcam, ab181869) was used in western blot on mouse samples (fig 8). Cell Death Dis (2017) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 4c
Invitrogen Vamp2 antibody (Affinity Bioreagents, PA1 766) was used in immunoprecipitation on human samples (fig 4c). J Biol Chem (2017) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:3000; fig 2
In order to study creatine kinase microcompartments and energy metabolism that recruits B-type creatine kinase to specific subcellular sites, Invitrogen Vamp2 antibody (Pierce, PA1-766) was used in western blot on rat samples at 1:3000 (fig 2). Amino Acids (2016) ncbi
domestic rabbit polyclonal
In order to identify alterations in the islet proteome that characterize the adaptive response, Invitrogen Vamp2 antibody (Thermo Fisher, PA1-766) was used . J Proteome Res (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-11)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 2e
Santa Cruz Biotechnology Vamp2 antibody (SantaCruz, sc-133129) was used in immunocytochemistry on mouse samples at 1:100 (fig 2e). J Immunol Methods (2017) ncbi
mouse monoclonal (4E240)
  • western blot; mouse; fig 1
Santa Cruz Biotechnology Vamp2 antibody (Santa Cruz, sc58309) was used in western blot on mouse samples (fig 1). Diabetes (2013) ncbi
Alomone Labs
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 5k
Alomone Labs Vamp2 antibody (Alomone labs, ANR-007) was used in immunohistochemistry on mouse samples at 1:1000 (fig 5k). Nat Commun (2021) ncbi
Articles Reviewed
  1. Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
  2. Zhang X, Liu Y, Hong X, Li X, Meshul C, Moore C, et al. NG2 glia-derived GABA release tunes inhibitory synapses and contributes to stress-induced anxiety. Nat Commun. 2021;12:5740 pubmed publisher
  3. Ivanova D, Dobson K, Gajbhiye A, Davenport E, Hacker D, Ultanir S, et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv. 2021;7: pubmed publisher
  4. Vevea J, Chapman E. Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. elife. 2020;9: pubmed publisher
  5. Yadirgi G, Stickings P, Rajagopal S, Liu Y, Sesardic D. Immuno-detection of cleaved SNAP-25 from differentiated mouse embryonic stem cells provides a sensitive assay for determination of botulinum A toxin and antitoxin potency. J Immunol Methods. 2017;451:90-99 pubmed publisher
  6. Brasher M, Martynowicz D, Grafinger O, Hucik A, Shanks Skinner E, Uniacke J, et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem. 2017;292:16199-16210 pubmed publisher
  7. Gümürdü A, Yildiz R, Eren E, Karakülah G, Unver T, Genc S, et al. MicroRNA exocytosis by large dense-core vesicle fusion. Sci Rep. 2017;7:45661 pubmed publisher
  8. Santos T, Wierda K, Broeke J, Toonen R, Verhage M. Early Golgi Abnormalities and Neurodegeneration upon Loss of Presynaptic Proteins Munc18-1, Syntaxin-1, or SNAP-25. J Neurosci. 2017;37:4525-4539 pubmed publisher
  9. Han F, Liu C, Zhang L, Zhou Y, Qin Y, Wang Y, et al. Globozoospermia and lack of acrosome formation in GM130-deficient mice. Cell Death Dis. 2017;8:e2532 pubmed publisher
  10. Sclip A, Bacaj T, Giam L, Sudhof T. Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction. PLoS ONE. 2016;11:e0158295 pubmed publisher
  11. Schlattner U, Klaus A, Ramírez Ríos S, Guzun R, Kay L, Tokarska Schlattner M. Cellular compartmentation of energy metabolism: creatine kinase microcompartments and recruitment of B-type creatine kinase to specific subcellular sites. Amino Acids. 2016;48:1751-74 pubmed publisher
  12. Bacaj T, Wu D, Burré J, Malenka R, Liu X, Sudhof T. Synaptotagmin-1 and -7 Are Redundantly Essential for Maintaining the Capacity of the Readily-Releasable Pool of Synaptic Vesicles. PLoS Biol. 2015;13:e1002267 pubmed publisher
  13. El Ouaamari A, Zhou J, Liew C, Shirakawa J, Dirice E, Gedeon N, et al. Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics. J Proteome Res. 2015;14:3111-3122 pubmed publisher
  14. Lee S, Sharma M, S dhof T, Shen J. Synaptic function of nicastrin in hippocampal neurons. Proc Natl Acad Sci U S A. 2014;111:8973-8 pubmed publisher
  15. Song W, Mondal P, Li Y, Lee S, Hussain M. Pancreatic ?-cell response to increased metabolic demand and to pharmacologic secretagogues requires EPAC2A. Diabetes. 2013;62:2796-807 pubmed publisher