This is a Validated Antibody Database (VAD) review about rat Trim28, based on 19 published articles (read how Labome selects the articles), using Trim28 antibody in all methods. It is aimed to help Labome visitors find the most suited Trim28 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Trim28 synonym: Kap1; Krip1; Tif1b

Knockout validation
Abcam
mouse monoclonal (20C1)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 3c
Abcam Trim28 antibody (Abcam, ab22553) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 3c). Stem Cell Reports (2020) ncbi
Abcam
mouse monoclonal (20C1)
  • western blot; human; loading ...; fig 2c, 2h
Abcam Trim28 antibody (Abcam, Ab22553) was used in western blot on human samples (fig 2c, 2h). Nucleic Acids Res (2020) ncbi
mouse monoclonal (20C1)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 3c
Abcam Trim28 antibody (Abcam, ab22553) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 3c). Stem Cell Reports (2020) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2a
Abcam Trim28 antibody (Abcam, 20C1) was used in immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 2a). PLoS Pathog (2019) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; mouse; 1:1000; loading ...; fig 4b
Abcam Trim28 antibody (Abcam, ab22553) was used in immunoprecipitation on mouse samples at 1:1000 (fig 4b). Nat Commun (2019) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3a
Abcam Trim28 antibody (Abcam, ab22553) was used in immunoprecipitation on human samples (fig 3d) and in western blot on human samples (fig 3a). Proc Natl Acad Sci U S A (2019) ncbi
mouse monoclonal (20C1)
  • western blot; human; loading ...; fig 2d
Abcam Trim28 antibody (Abcam, ab22553) was used in western blot on human samples (fig 2d). Sci Signal (2018) ncbi
mouse monoclonal (20C1)
  • immunocytochemistry; mouse; 1:500
  • western blot; mouse; 1:1000
In order to characterize TRIM28 as an epigenetic barrier to generate reprogramming of pluripotent stem cells, Abcam Trim28 antibody (Abcam, ab22553) was used in immunocytochemistry on mouse samples at 1:500 and in western blot on mouse samples at 1:1000. Stem Cells (2017) ncbi
mouse monoclonal (20C1)
  • EMSA; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 5a
Abcam Trim28 antibody (Abcam, ab22553) was used in EMSA on human samples (fig 4a) and in western blot on human samples (fig 5a). PLoS ONE (2015) ncbi
mouse monoclonal (20C1)
  • western blot; mouse
Abcam Trim28 antibody (Abcam, ab22553) was used in western blot on mouse samples . DNA Res (2015) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; human; fig s3e
  • immunocytochemistry; human; 1:500; fig s4b
  • western blot; human; fig s3c
Abcam Trim28 antibody (Abcam, ab22553) was used in immunoprecipitation on human samples (fig s3e), in immunocytochemistry on human samples at 1:500 (fig s4b) and in western blot on human samples (fig s3c). Cell Rep (2015) ncbi
mouse monoclonal (20C1)
  • chromatin immunoprecipitation; mouse
Abcam Trim28 antibody (Abcam, ab22553) was used in chromatin immunoprecipitation on mouse samples . J Virol (2014) ncbi
mouse monoclonal (20C1)
  • western blot; human
Abcam Trim28 antibody (Abcam, ab22553) was used in western blot on human samples . PLoS ONE (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C42G12)
  • western blot; mouse; loading ...; fig 3c
Cell Signaling Technology Trim28 antibody (Cell Signaling Technologies, 4124S) was used in western blot on mouse samples (fig 3c). Cell Rep (2021) ncbi
domestic rabbit monoclonal (C42G12)
  • western blot; human; 1:1000; loading ...; fig s4a
Cell Signaling Technology Trim28 antibody (Cell Signaling Technology, 4124) was used in western blot on human samples at 1:1000 (fig s4a). Front Oncol (2020) ncbi
domestic rabbit monoclonal (C42G12)
  • western blot; human; 1:500; loading ...; fig 5b
Cell Signaling Technology Trim28 antibody (Cell Signaling, 4124S) was used in western blot on human samples at 1:500 (fig 5b). RNA (2021) ncbi
domestic rabbit monoclonal (C42G12)
  • immunoprecipitation; human; fig 3h
Cell Signaling Technology Trim28 antibody (Cell Signaling technology, 4124S) was used in immunoprecipitation on human samples (fig 3h). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1a
Cell Signaling Technology Trim28 antibody (Cell Signaling, 4123) was used in western blot on human samples (fig 1a). Mol Cell (2019) ncbi
domestic rabbit monoclonal (C42G12)
  • immunocytochemistry; mouse; 1:1000; fig s6
  • western blot; mouse; fig s3
Cell Signaling Technology Trim28 antibody (Cell Signaling, 4124) was used in immunocytochemistry on mouse samples at 1:1000 (fig s6) and in western blot on mouse samples (fig s3). Nat Struct Mol Biol (2015) ncbi
BD Biosciences
mouse monoclonal (23/KRIP1)
  • western blot; human; fig 2i
BD Biosciences Trim28 antibody (BD Biosciences, 610680) was used in western blot on human samples (fig 2i). J Biol Chem (2015) ncbi
Articles Reviewed
  1. Steinert N, Potts G, Wilson G, Klamen A, Lin K, Hermanson J, et al. Mapping of the contraction-induced phosphoproteome identifies TRIM28 as a significant regulator of skeletal muscle size and function. Cell Rep. 2021;34:108796 pubmed publisher
  2. Fang M, Zhang M, Wang Y, Wei F, Wu J, Mou X, et al. Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity. Front Oncol. 2020;10:601055 pubmed publisher
  3. Indacochea A, Guerrero S, Ure xf1 a M, Araujo F, Coll O, Lleonart M, et al. Cold-inducible RNA binding protein promotes breast cancer cell malignancy by regulating Cystatin C levels. RNA. 2021;27:190-201 pubmed publisher
  4. Hu H, Ji Q, Song M, Ren J, Liu Z, Wang Z, et al. ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin. Nucleic Acids Res. 2020;48:6001-6018 pubmed publisher
  5. Tan J, Wollmann H, van Pelt A, Kaldis P, Messerschmidt D. Infertility-Causing Haploinsufficiency Reveals TRIM28/KAP1 Requirement in Spermatogonia. Stem Cell Reports. 2020;14:818-827 pubmed publisher
  6. Ding L, Zhu Q, Zhou F, Tan H, Xu W, Pan C, et al. Identification of viral SIM-SUMO2-interaction inhibitors for treating primary effusion lymphoma. PLoS Pathog. 2019;15:e1008174 pubmed publisher
  7. Peter C, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun. 2019;10:4112 pubmed publisher
  8. Colomer C, Margalef P, Villanueva A, Vert A, Pecharroman I, Sole L, et al. IKKα Kinase Regulates the DNA Damage Response and Drives Chemo-resistance in Cancer. Mol Cell. 2019;75:669-682.e5 pubmed publisher
  9. Chen W, Schwalie P, Pankevich E, Gubelmann C, Raghav S, Dainese R, et al. ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer. Nat Commun. 2019;10:1809 pubmed publisher
  10. Taura M, Song E, Ho Y, Iwasaki A. Apobec3A maintains HIV-1 latency through recruitment of epigenetic silencing machinery to the long terminal repeat. Proc Natl Acad Sci U S A. 2019;116:2282-2289 pubmed publisher
  11. Lee J, Mand M, Kao C, Zhou Y, Ryu S, Richards A, et al. ATM directs DNA damage responses and proteostasis via genetically separable pathways. Sci Signal. 2018;11: pubmed publisher
  12. Miles D, de Vries N, Gisler S, Lieftink C, Akhtar W, Gogola E, et al. TRIM28 is an Epigenetic Barrier to Induced Pluripotent Stem Cell Reprogramming. Stem Cells. 2017;35:147-157 pubmed publisher
  13. Guo Y, Feng W, Sy S, Huen M. ATM-dependent Phosphorylation of the Fanconi Anemia Protein PALB2 Promotes the DNA Damage Response. J Biol Chem. 2015;290:27545-56 pubmed publisher
  14. Ichida Y, Utsunomiya Y, Yasuda T, Nakabayashi K, Sato T, Onodera M. Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing. PLoS ONE. 2015;10:e0139274 pubmed publisher
  15. Akiyama T, Xin L, Oda M, Sharov A, Amano M, Piao Y, et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res. 2015;22:307-18 pubmed publisher
  16. Hartlerode A, Morgan M, Wu Y, Buis J, Ferguson D. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat Struct Mol Biol. 2015;22:736-43 pubmed publisher
  17. Xu Y, Zhang H, Nguyen V, Angelopoulos N, Nunes J, Reid A, et al. LMTK3 Represses Tumor Suppressor-like Genes through Chromatin Remodeling in Breast Cancer. Cell Rep. 2015;12:837-49 pubmed publisher
  18. Kao T, Liao H, Wolf D, Tai K, Chuang C, Lee H, et al. Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing in somatic cells. J Virol. 2014;88:10680-95 pubmed publisher
  19. Lee J, Guo Z, Myler L, Zheng S, Paull T. Direct activation of ATM by resveratrol under oxidizing conditions. PLoS ONE. 2014;9:e97969 pubmed publisher