This is a Validated Antibody Database (VAD) review about rat Tln1, based on 37 published articles (read how Labome selects the articles), using Tln1 antibody in all methods. It is aimed to help Labome visitors find the most suited Tln1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Tln1 synonym: Tln

Knockout validation
MilliporeSigma
mouse monoclonal (8d4)
  • western blot knockout validation; mouse; 1:1000; fig 1
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, MilliporeSigma Tln1 antibody (Sigma, 8D4) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). elife (2016) ncbi
Abcam
mouse monoclonal (8d4)
  • flow cytometry; mouse; 1:200; loading ...; fig s10c
Abcam Tln1 antibody (Abcam, ab157808) was used in flow cytometry on mouse samples at 1:200 (fig s10c). Nat Commun (2020) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; loading ...; fig 4
Abcam Tln1 antibody (Abcam, ab11188) was used in immunocytochemistry on human samples (fig 4). J Extracell Vesicles (2017) ncbi
mouse monoclonal (8d4)
  • western blot; human; 1:1000
In order to study vinculin activation and function, Abcam Tln1 antibody (Abcam, ab11188) was used in western blot on human samples at 1:1000. Nat Cell Biol (2015) ncbi
mouse monoclonal (8d4)
  • western blot; human; 1:500
Abcam Tln1 antibody (Abcam, ab11188) was used in western blot on human samples at 1:500. Stem Cell Rev (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-9)
  • western blot; human; 1:200; loading ...; fig 2d
Santa Cruz Biotechnology Tln1 antibody (Santa Cruz, sc-365875) was used in western blot on human samples at 1:200 (fig 2d). Exp Cell Res (2018) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C45F1)
  • western blot; mouse; 1:1000; loading ...; fig 4a
Cell Signaling Technology Tln1 antibody (Cell Signaling, 4021) was used in western blot on mouse samples at 1:1000 (fig 4a). Commun Biol (2021) ncbi
domestic rabbit monoclonal (C45F1)
  • immunocytochemistry; human; 1:100; loading ...; fig 5g
  • western blot; human; 1:1000; loading ...; fig 5c
Cell Signaling Technology Tln1 antibody (Cell Signaling Technology, 4021) was used in immunocytochemistry on human samples at 1:100 (fig 5g) and in western blot on human samples at 1:1000 (fig 5c). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:400; loading ...; fig 3a
  • western blot; mouse; 1:400; loading ...; fig 3a
Cell Signaling Technology Tln1 antibody (Cell Signaling, 5426) was used in western blot on human samples at 1:400 (fig 3a) and in western blot on mouse samples at 1:400 (fig 3a). Nitric Oxide (2018) ncbi
MilliporeSigma
mouse monoclonal (8d4)
  • immunocytochemistry; Xenopus laevis; 1:100; loading ...; fig 1s2b
MilliporeSigma Tln1 antibody (Biolegend, T3287) was used in immunocytochemistry on Xenopus laevis samples at 1:100 (fig 1s2b). elife (2020) ncbi
mouse monoclonal (8d4)
  • immunohistochemistry; human; 1:250; loading ...; fig 3a
  • western blot; human; 1:2000; loading ...; fig 3d
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in immunohistochemistry on human samples at 1:250 (fig 3a) and in western blot on human samples at 1:2000 (fig 3d). elife (2019) ncbi
mouse monoclonal (8d4)
  • western blot; human; 1:2000; loading ...; fig 5b
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on human samples at 1:2000 (fig 5b). J Cell Sci (2019) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; loading ...; fig 4g
MilliporeSigma Tln1 antibody (Sigma-Aldrich, T3287) was used in western blot on mouse samples (fig 4g). Immunity (2019) ncbi
mouse monoclonal (8d4)
  • western blot; human; 1:1000; loading ...; fig 1a
MilliporeSigma Tln1 antibody (Sigma, 8d4) was used in western blot on human samples at 1:1000 (fig 1a). J Cell Biol (2018) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; loading ...; fig 4f
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on mouse samples at 1:1000 (fig 4f). Arterioscler Thromb Vasc Biol (2017) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; loading ...; fig 4k
MilliporeSigma Tln1 antibody (Sigma-Aldrich, 8d4) was used in western blot on mouse samples (fig 4k). J Cell Biol (2017) ncbi
mouse monoclonal (8d4)
  • immunohistochemistry; human; 1:250; loading ...; fig st14
  • immunohistochemistry; chicken; 1:250; loading ...; fig st14
  • immunohistochemistry; mouse; 1:250; loading ...; fig st14
  • immunohistochemistry; rat; 1:250; loading ...; fig st14
In order to outline the protocols for antibodies used for immunohistochemical studies, MilliporeSigma Tln1 antibody (Sigma-Aldrich, T3287) was used in immunohistochemistry on human samples at 1:250 (fig st14), in immunohistochemistry on chicken samples at 1:250 (fig st14), in immunohistochemistry on mouse samples at 1:250 (fig st14) and in immunohistochemistry on rat samples at 1:250 (fig st14). J Toxicol Pathol (2017) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; loading ...; fig s1d
In order to measure the adhesion of alphaV-class and/or alpha5beta1 integrins expressing fibroblasts initiating attachment to fibronectin, MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on mouse samples at 1:1000 (fig s1d). Nat Commun (2017) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; 1:500; fig 3b
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in immunocytochemistry on human samples at 1:500 (fig 3b). Mol Med Rep (2016) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; loading ...; fig 1e
  • western blot; human; loading ...; fig 9a
In order to examine the role of myocardin-related transcription factor overexpression in cell migration, MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig 1e) and in western blot on human samples (fig 9a). Oncotarget (2016) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; fig 1
MilliporeSigma Tln1 antibody (Sigma, 8d4) was used in immunocytochemistry on human samples (fig 1). elife (2016) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; guinea pig; 1:100; fig 1
  • western blot; guinea pig; 1:1000; fig 1
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in immunocytochemistry on guinea pig samples at 1:100 (fig 1) and in western blot on guinea pig samples at 1:1000 (fig 1). Biol Open (2016) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; fig 4
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in immunocytochemistry on human samples (fig 4). Oncogene (2016) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; fig s4
In order to study the response to matrix rigidity by mechanical regulation of a molecular clutch that defines force transmission and transduction, MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on mouse samples at 1:1000 (fig s4). Nat Cell Biol (2016) ncbi
mouse monoclonal (8d4)
  • immunohistochemistry; human; 1:100; loading ...; fig 6d
  • western blot; human; 1:1000; loading ...; fig 6a
In order to investigate how recycled integrins assemble into new adhesions, MilliporeSigma Tln1 antibody (SigmaAldrich, 8d4) was used in immunohistochemistry on human samples at 1:100 (fig 6d) and in western blot on human samples at 1:1000 (fig 6a). Nat Cell Biol (2016) ncbi
mouse monoclonal (8d4)
  • western blot knockout validation; mouse; 1:1000; fig 1
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, MilliporeSigma Tln1 antibody (Sigma, 8D4) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). elife (2016) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; fig s1
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig s1). Nat Commun (2015) ncbi
mouse monoclonal (8d4)
  • western blot; rat; 1:800; fig 2
In order to report that Thy-1 is a regulator of fibroblast rigidity sensing, MilliporeSigma Tln1 antibody (Sigma-Aldrich, 8d4) was used in western blot on rat samples at 1:800 (fig 2). J Cell Biol (2015) ncbi
mouse monoclonal (8d4)
  • western blot; human; fig 1
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on human samples (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; fig 4
MilliporeSigma Tln1 antibody (Sigma, T3287) was used in immunocytochemistry on human samples (fig 4). Nat Commun (2015) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:500
MilliporeSigma Tln1 antibody (Sigma-Aldrich, clone 8d4) was used in immunocytochemistry on human samples at 1:100 and in western blot on human samples at 1:500. Integr Biol (Camb) (2015) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; fig 1
In order to study phosphorylation events at sites of adhesion, MilliporeSigma Tln1 antibody (Sigma-Aldrich, T3287) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (8d4)
  • western blot; human
In order to examine the regulation of integrin-containing adhesion complexes by the microtubule network, MilliporeSigma Tln1 antibody (Sigma-Aldrich, 8D4) was used in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human
In order to study leukocyte function-associated antigen 1 signaling with a focus on integrins, MilliporeSigma Tln1 antibody (Sigma-Aldrich, 8d4) was used in immunocytochemistry on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human
  • western blot; human
MilliporeSigma Tln1 antibody (Sigma-Aldrich, 8d4) was used in immunocytochemistry on human samples and in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; fig 1
In order to study sorting nexin (SNX) family members interactions with beta integrins, MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig 1). J Mol Biol (2014) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; fig 5, 7
In order to test if loss of Vcl and ZO-1 interactions destabilizes gap junctions in cardiomyocytes, MilliporeSigma Tln1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig 5, 7). J Cell Sci (2014) ncbi
mouse monoclonal (8d4)
  • western blot; hamsters; 1:2,000
MilliporeSigma Tln1 antibody (Sigma-Aldrich, 8D4) was used in western blot on hamsters samples at 1:2,000. PLoS ONE (2013) ncbi
Articles Reviewed
  1. Qin L, Fu X, Ma J, Lin M, Zhang P, Wang Y, et al. Kindlin-2 mediates mechanotransduction in bone by regulating expression of Sclerostin in osteocytes. Commun Biol. 2021;4:402 pubmed publisher
  2. Liu Y, Li L, Liu X, Wang Y, Liu L, Peng L, et al. Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling. elife. 2020;9: pubmed publisher
  3. Chan Z, Kwan H, Wong Y, Jiang Z, Zhou Z, Tam K, et al. Site-directed MT1-MMP trafficking and surface insertion regulate AChR clustering and remodeling at developing NMJs. elife. 2020;9: pubmed publisher
  4. Martens R, Permanyer M, Werth K, Yu K, Braun A, Halle O, et al. Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance. Nat Commun. 2020;11:1114 pubmed publisher
  5. Bayer S, Grither W, Brenot A, Hwang P, Barcus C, Ernst M, et al. DDR2 controls breast tumor stiffness and metastasis by regulating integrin mediated mechanotransduction in CAFs. elife. 2019;8: pubmed publisher
  6. Song K, Gras C, Capin G, Gimber N, Lehmann M, Mohd S, et al. A SEPT1-based scaffold is required for Golgi integrity and function. J Cell Sci. 2019;132: pubmed publisher
  7. Lin C, Zhang Y, Zhang K, Zheng Y, Lu L, Chang H, et al. Fever Promotes T Lymphocyte Trafficking via a Thermal Sensory Pathway Involving Heat Shock Protein 90 and α4 Integrins. Immunity. 2019;50:137-151.e6 pubmed publisher
  8. Huang Z, Barker D, Gibbins J, Dash P. Talin is a substrate for SUMOylation in migrating cancer cells. Exp Cell Res. 2018;370:417-425 pubmed publisher
  9. Zhan R, Wang F, Wu Y, Wang Y, Qian W, Liu M, et al. Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin β1 and Talin via the cGMP signalling pathway. Nitric Oxide. 2018;78:1-10 pubmed publisher
  10. Sun H, Lagarrigue F, Gingras A, Fan Z, Ley K, Ginsberg M. Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development. J Cell Biol. 2018;217:1453-1465 pubmed publisher
  11. Margraf A, Nussbaum C, Rohwedder I, Klapproth S, Kurz A, Florian A, et al. Maturation of Platelet Function During Murine Fetal Development In Vivo. Arterioscler Thromb Vasc Biol. 2017;37:1076-1086 pubmed publisher
  12. Johnson S, Dempsey C, Parker C, Mironov A, Bradley H, Saha V. Acute lymphoblastic leukaemia cells produce large extracellular vesicles containing organelles and an active cytoskeleton. J Extracell Vesicles. 2017;6:1294339 pubmed publisher
  13. Georgiadou M, Lilja J, Jacquemet G, Guzmán C, Rafaeva M, Alibert C, et al. AMPK negatively regulates tensin-dependent integrin activity. J Cell Biol. 2017;216:1107-1121 pubmed publisher
  14. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  15. Bharadwaj M, Strohmeyer N, Colo G, Helenius J, Beerenwinkel N, Schiller H, et al. αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun. 2017;8:14348 pubmed publisher
  16. Zeng F, Xie Y, Liao L, Li L, Chen B, Xie J, et al. Biological characterization of three immortalized esophageal epithelial cell lines. Mol Med Rep. 2016;14:4802-4810 pubmed publisher
  17. Kishi T, Mayanagi T, Iwabuchi S, Akasaka T, Sobue K. Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity. Oncotarget. 2016;7:72113-72130 pubmed publisher
  18. Bouchet B, Gough R, Ammon Y, van de Willige D, Post H, Jacquemet G, et al. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. elife. 2016;5: pubmed publisher
  19. Roa Espitia A, Hernández Rendón E, Baltiérrez Hoyos R, Muñoz Gotera R, Cote Vélez A, Jiménez I, et al. Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation. Biol Open. 2016;5:1189-99 pubmed publisher
  20. Cross A, Wilson A, Guerrero M, Thomas K, Bachir A, Kubow K, et al. Breast cancer antiestrogen resistance 3-p130Cas interactions promote adhesion disassembly and invasion in breast cancer cells. Oncogene. 2016;35:5850-5859 pubmed publisher
  21. Elosegui Artola A, Oria R, Chen Y, Kosmalska A, Pérez González C, Castro N, et al. Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity. Nat Cell Biol. 2016;18:540-8 pubmed publisher
  22. Nader G, Ezratty E, Gundersen G. FAK, talin and PIPKI? regulate endocytosed integrin activation to polarize focal adhesion assembly. Nat Cell Biol. 2016;18:491-503 pubmed publisher
  23. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  24. Atherton P, Stutchbury B, Wang D, Jethwa D, Tsang R, Meiler Rodriguez E, et al. Vinculin controls talin engagement with the actomyosin machinery. Nat Commun. 2015;6:10038 pubmed publisher
  25. Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
  26. Roda D, Castillo J, Telechea Fernández M, Gil A, López Rodas G, Franco L, et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS ONE. 2015;10:e0130543 pubmed publisher
  27. Case L, Baird M, Shtengel G, Campbell S, Hess H, Davidson M, et al. Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions. Nat Cell Biol. 2015;17:880-92 pubmed publisher
  28. Poulter N, Pollitt A, Davies A, Malinova D, Nash G, Hannon M, et al. Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex. Nat Commun. 2015;6:7254 pubmed publisher
  29. Kiss A, Gong X, Kowalewski J, Shafqat Abbasi H, Strömblad S, Lock J. Non-monotonic cellular responses to heterogeneity in talin protein expression-level. Integr Biol (Camb). 2015;7:1171-85 pubmed publisher
  30. Robertson J, Jacquemet G, Byron A, Jones M, Warwood S, Selley J, et al. Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun. 2015;6:6265 pubmed publisher
  31. Ng D, Humphries J, Byron A, Millon Frémillon A, Humphries M. Microtubule-dependent modulation of adhesion complex composition. PLoS ONE. 2014;9:e115213 pubmed publisher
  32. Uotila L, Jahan F, Soto Hinojosa L, Melandri E, Grönholm M, Gahmberg C. Specific phosphorylations transmit signals from leukocyte β2 to β1 integrins and regulate adhesion. J Biol Chem. 2014;289:32230-42 pubmed publisher
  33. Abramowski P, Ogrodowczyk C, Martin R, Pongs O. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS ONE. 2014;9:e104692 pubmed publisher
  34. Tseng H, Thorausch N, Ziegler T, Meves A, Fassler R, Böttcher R. Sorting nexin 31 binds multiple ? integrin cytoplasmic domains and regulates ?1 integrin surface levels and stability. J Mol Biol. 2014;426:3180-3194 pubmed publisher
  35. Groenendyk J, Michalak M. Disrupted WNT signaling in mouse embryonic stem cells in the absence of calreticulin. Stem Cell Rev. 2014;10:191-206 pubmed publisher
  36. Zemljic Harpf A, Godoy J, Platoshyn O, Asfaw E, Busija A, Domenighetti A, et al. Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes. J Cell Sci. 2014;127:1104-16 pubmed publisher
  37. Honda S, Shirotani Ikejima H, Tadokoro S, Tomiyama Y, Miyata T. The integrin-linked kinase-PINCH-parvin complex supports integrin ?IIb?3 activation. PLoS ONE. 2013;8:e85498 pubmed publisher