This is a Validated Antibody Database (VAD) review about rat Syngap1, based on 11 published articles (read how Labome selects the articles), using Syngap1 antibody in all methods. It is aimed to help Labome visitors find the most suited Syngap1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Syngap1 synonym: Syngap; ras/Rap GTPase-activating protein SynGAP; neuronal RasGAP; p135 SynGAP; ras GTPase-activating protein SynGAP; synaptic Ras-GAP 1

Invitrogen
rabbit polyclonal
  • western blot; scFv; 1:10,000; loading ...
Invitrogen Syngap1 antibody (Pierce/Thermo Scientific, PA1-046) was used in western blot on scFv samples at 1:10,000. elife (2019) ncbi
rabbit polyclonal
  • western blot; mouse; 1:3500; fig 11
In order to demonstrate that synGAP-alpha1 regulates postsynaptic density composition by restricting binding to the PDZ domains of PSD-95, Invitrogen Syngap1 antibody (Pierce, PA1-046) was used in western blot on mouse samples at 1:3500 (fig 11). elife (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig s4
In order to determine regulation of spine structural plasticity and memory and learning by the calcium sensor Copine-6, Invitrogen Syngap1 antibody (Affinity Bioreagents, PA1046) was used in western blot on mouse samples at 1:1000 (fig s4). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:1000
  • western blot; mouse; 1:1000
In order to assess the role of the GluN2B subunit during development and in synaptic plasticity, Invitrogen Syngap1 antibody (Affinity Bioreagents, PA1-046) was used in immunocytochemistry on mouse samples at 1:1000 and in western blot on mouse samples at 1:1000. J Neurosci (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; 8 ug/time
  • western blot; mouse; 1:1000
In order to study the effect of phosphorylation on synGAP activity, Invitrogen Syngap1 antibody (Thermo Scientific, PA1-046) was used in immunoprecipitation on mouse samples at 8 ug/time and in western blot on mouse samples at 1:1000. J Biol Chem (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:1,000
In order to examine the progression of functional corticostriatal interactions, Invitrogen Syngap1 antibody (Pierce, PA1-046) was used in immunohistochemistry on rat samples at 1:1,000. J Comp Neurol (2015) ncbi
rabbit polyclonal
In order to study the effect of activity on synapse composition and organization in central nerve system, Invitrogen Syngap1 antibody (Affinity BioReagents, PA1-046) was used . J Comp Neurol (2005) ncbi
Cell Signaling Technology
rabbit monoclonal (D88G1)
  • western blot; mouse; 1:1000; loading ...; fig 6c
Cell Signaling Technology Syngap1 antibody (Cell Signaling, 9479) was used in western blot on mouse samples at 1:1000 (fig 6c). Nat Neurosci (2018) ncbi
rabbit monoclonal (D20C7)
  • immunoprecipitation; mouse; loading ...; fig 2a
  • western blot; mouse; 1:1000; loading ...; fig 2a
In order to study the role of GAP and GEF complexing in GTP signaling, Cell Signaling Technology Syngap1 antibody (Cell Signaling, 5539) was used in immunoprecipitation on mouse samples (fig 2a) and in western blot on mouse samples at 1:1000 (fig 2a). Sci Rep (2017) ncbi
rabbit monoclonal (D78B11)
  • western blot; mouse; fig s2c
In order to present the effect of pharmacological enhancement of mGlu5 receptors on behavioral deficits in SHANK3 knock-out mice, Cell Signaling Technology Syngap1 antibody (Cell Signaling, D78B11) was used in western blot on mouse samples (fig s2c). Mol Psychiatry (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3c
In order to demonstrate that the dietary glycemic index has a significant impact on the autism spectrum disorder phenotype, Cell Signaling Technology Syngap1 antibody (Cell Signaling Technology, 3200) was used in western blot on mouse samples at 1:1000 (fig 3c). Mol Psychiatry (2016) ncbi
Articles Reviewed
  1. Creson T, Rojas C, Hwaun E, Vaissière T, Kilinc M, Jimenez Gomez A, et al. Re-expression of SynGAP protein in adulthood improves translatable measures of brain function and behavior. elife. 2019;8: pubmed publisher
  2. Qin L, Ma K, Wang Z, Hu Z, Matas E, Wei J, et al. Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition. Nat Neurosci. 2018;21:564-575 pubmed publisher
  3. Wilkinson B, Li J, Coba M. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep. 2017;7:5272 pubmed publisher
  4. Walkup W, Mastro T, Schenker L, Vielmetter J, Hu R, Iancu A, et al. A model for regulation by SynGAP-?1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic density. elife. 2016;5: pubmed publisher
  5. Reinhard J, Kriz A, Galic M, Angliker N, Rajalu M, Vogt K, et al. The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Nat Commun. 2016;7:11613 pubmed publisher
  6. Vicidomini C, Ponzoni L, Lim D, Schmeisser M, Reim D, Morello N, et al. Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice. Mol Psychiatry. 2017;22:689-702 pubmed publisher
  7. Currais A, Farrokhi C, Dargusch R, Goujon Svrzic M, Maher P. Dietary glycemic index modulates the behavioral and biochemical abnormalities associated with autism spectrum disorder. Mol Psychiatry. 2016;21:426-36 pubmed publisher
  8. Ferreira J, Schmidt J, Rio P, Águas R, Rooyakkers A, Li K, et al. GluN2B-Containing NMDA Receptors Regulate AMPA Receptor Traffic through Anchoring of the Synaptic Proteasome. J Neurosci. 2015;35:8462-79 pubmed publisher
  9. Walkup W, Washburn L, Sweredoski M, Carlisle H, Graham R, Hess S, et al. Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases. J Biol Chem. 2015;290:4908-27 pubmed publisher
  10. Friedman L, Riemslagh F, Sullivan J, Mesias R, Williams F, Huntley G, et al. Cadherin-8 expression, synaptic localization, and molecular control of neuronal form in prefrontal corticostriatal circuits. J Comp Neurol. 2015;523:75-92 pubmed publisher
  11. Harms K, Craig A. Synapse composition and organization following chronic activity blockade in cultured hippocampal neurons. J Comp Neurol. 2005;490:72-84 pubmed