This is a Validated Antibody Database (VAD) review about rat Srsf3, based on 16 published articles (read how Labome selects the articles), using Srsf3 antibody in all methods. It is aimed to help Labome visitors find the most suited Srsf3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Srsf3 synonym: Sfrs3; serine/arginine-rich splicing factor 3; splicing factor, arginine/serine-rich 3 (SRp20)

Invitrogen
mouse monoclonal (16H3 (16H3E8))
  • immunocytochemistry; human; 1:200; fig 3
In order to examine the methylation state of ATXN2L and its contribution to ATXN2L localization, Invitrogen Srsf3 antibody (Life Technologies, 16H3) was used in immunocytochemistry on human samples at 1:200 (fig 3). Exp Cell Res (2015) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to report that Thoc5 and CFIm68 interact, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in western blot on human samples (fig 1). Nucleic Acids Res (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to explore the mechanism underlying paraquat-induced pre-mRNA splicing alterations, Invitrogen Srsf3 antibody (Invitrogen, clone 16H3) was used in western blot on human samples (fig 1). PLoS ONE (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; rat; fig 3
In order to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA, Invitrogen Srsf3 antibody (Invitrogen, clone 16H3) was used in western blot on rat samples (fig 3). J Biol Chem (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:100; fig 3
  • western blot; mouse; 1:100; fig 3
In order to assess the splicing machinery in Down syndrome brains, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in western blot on human samples at 1:100 (fig 3) and in western blot on mouse samples at 1:100 (fig 3). Neurobiol Dis (2010) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to identify factors that modulate TF pre-mRNA splicing, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in western blot on human samples (fig 1). J Leukoc Biol (2010) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 3
In order to examine the effect of extracellular pH on SMN2 exon 7 splicing in spinal muscular atrophy cells, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in western blot on human samples (fig 3). Mol Cell Neurosci (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; mouse; fig 2
  • western blot; human; 1:2000; fig 1
In order to assess how SR kinases play a role in alternative splicing through dynamic balance of phosphorylation, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in western blot on mouse samples (fig 2) and in western blot on human samples at 1:2000 (fig 1). Genes Cells (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • immunoprecipitation; human; fig 4
In order to determine whether mutations within 5' splice site downstream of exon2 result in reduced or increased HIV-1 Vif expression, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in immunoprecipitation on human samples (fig 4). J Virol (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 2
In order to determine the effect of hydroxyurea in spinal muscular atrophy cells and patients, Invitrogen Srsf3 antibody (Zymed Laboratories, 16H3) was used in western blot on human samples (fig 2). J Neurol Sci (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:500
In order to investigate if inhibitors of the Na+/H+ exchanger modulate the exon 7 splicing of survival motor neuron 2 mRNA in spinal muscular atrophy patients, Invitrogen Srsf3 antibody (Zymed Laboratories, 16H3) was used in western blot on human samples at 1:500. Ann Neurol (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:1000
In order to determine the molecular mechanisms associated with ankyloblepharon, ectodermal dysplasia, and clefting, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in western blot on human samples at 1:1000. Cell Cycle (2005) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; mouse
In order to examine the therapeutic use of sodium butyrate to treat spinal muscular atrophy, Invitrogen Srsf3 antibody (Zymed, 16H3) was used in western blot on mouse samples . Proc Natl Acad Sci U S A (2001) ncbi
Santa Cruz Biotechnology
mouse monoclonal (7B4)
  • western blot; human
Santa Cruz Biotechnology Srsf3 antibody (Santa Cruz Biotechnology, sc-13510) was used in western blot on human samples . Mol Cancer (2015) ncbi
MilliporeSigma
mouse monoclonal (2D2)
  • western blot; mouse; 1:2000; loading ...; fig 4b
MilliporeSigma Srsf3 antibody (Sigma-Aldrich, 2D2) was used in western blot on mouse samples at 1:2000 (fig 4b). Nat Commun (2019) ncbi
mouse monoclonal (2D2)
  • western blot; human; loading ...; fig S1
MilliporeSigma Srsf3 antibody (Sigma-Aldrich, WH0006428M8) was used in western blot on human samples (fig S1). Nucleic Acids Res (2017) ncbi
Articles Reviewed
  1. Legrand J, Chan A, La H, Rossello F, Ankö M, Fuller Pace F, et al. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nat Commun. 2019;10:2278 pubmed publisher
  2. Kralovicova J, Vorechovsky I. Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons. Nucleic Acids Res. 2017;45:417-434 pubmed publisher
  3. He X, Zhang P. Serine/arginine-rich splicing factor 3 (SRSF3) regulates homologous recombination-mediated DNA repair. Mol Cancer. 2015;14:158 pubmed publisher
  4. Kaehler C, Guenther A, Uhlich A, Krobitsch S. PRMT1-mediated arginine methylation controls ATXN2L localization. Exp Cell Res. 2015;334:114-25 pubmed publisher
  5. Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y. Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Nucleic Acids Res. 2013;41:7060-72 pubmed publisher
  6. Vivarelli S, Lenzken S, Ruepp M, Ranzini F, Maffioletti A, Alvarez R, et al. Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2. PLoS ONE. 2013;8:e61980 pubmed publisher
  7. Walsh C, Suchanek A, Cyphert T, Kohan A, Szeszel Fedorowicz W, Salati L. Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver. J Biol Chem. 2013;288:2816-28 pubmed publisher
  8. Toiber D, Azkona G, Ben Ari S, Toran N, Soreq H, Dierssen M. Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations. Neurobiol Dis. 2010;40:348-59 pubmed publisher
  9. Chandradas S, Deikus G, Tardos J, Bogdanov V. Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 2010;87:147-52 pubmed publisher
  10. Chen Y, Yuo C, Yang W, Jong Y, Lin H, Chang Y, et al. Extracellular pH change modulates the exon 7 splicing in SMN2 mRNA. Mol Cell Neurosci. 2008;39:268-72 pubmed publisher
  11. Yomoda J, Muraki M, Kataoka N, Hosoya T, Suzuki M, Hagiwara M, et al. Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A. Genes Cells. 2008;13:233-44 pubmed publisher
  12. Exline C, Feng Z, Stoltzfus C. Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression. J Virol. 2008;82:3921-31 pubmed publisher
  13. Liang W, Yuo C, Chang J, Chen Y, Chang Y, Wang H, et al. The effect of hydroxyurea in spinal muscular atrophy cells and patients. J Neurol Sci. 2008;268:87-94 pubmed publisher
  14. Yuo C, Lin H, Chang Y, Yang W, Chang J. 5-(N-ethyl-N-isopropyl)-amiloride enhances SMN2 exon 7 inclusion and protein expression in spinal muscular atrophy cells. Ann Neurol. 2008;63:26-34 pubmed
  15. Huang Y, Kim Y, Li Z, Fomenkov T, Fomenkov A, Ratovitski E. AEC-associated p63 mutations lead to alternative splicing/protein stabilization of p63 and modulation of Notch signaling. Cell Cycle. 2005;4:1440-7 pubmed
  16. Chang J, Hsieh Li H, Jong Y, Wang N, Tsai C, Li H. Treatment of spinal muscular atrophy by sodium butyrate. Proc Natl Acad Sci U S A. 2001;98:9808-13 pubmed