This is a Validated Antibody Database (VAD) review about rat Srebf1, based on 34 published articles (read how Labome selects the articles), using Srebf1 antibody in all methods. It is aimed to help Labome visitors find the most suited Srebf1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Srebf1 synonym: ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1

Knockout validation
Novus Biologicals
mouse monoclonal (2A4)
  • western blot knockout validation; human; 1:1000; loading ...; fig 1c
Novus Biologicals Srebf1 antibody (Novus, NB600-582) was used in western blot knockout validation on human samples at 1:1000 (fig 1c). Front Pharmacol (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2A4)
  • western blot; mouse; loading ...; fig 4a
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc13551) was used in western blot on mouse samples (fig 4a). Mol Metab (2022) ncbi
mouse monoclonal (2A4)
  • western blot; mouse; 1:400; loading ...; fig 6b
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-13551) was used in western blot on mouse samples at 1:400 (fig 6b). Cell Death Dis (2021) ncbi
mouse monoclonal (A-4)
  • western blot; mouse; 1:1000; loading ...; fig s1a
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-365513) was used in western blot on mouse samples at 1:1000 (fig s1a). J Inflamm Res (2021) ncbi
mouse monoclonal (F-10)
  • western blot; human; 1:1000; loading ...; fig 4d
  • western blot; mouse; 1:1000; loading ...; fig 4a, 4c
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-365514) was used in western blot on human samples at 1:1000 (fig 4d) and in western blot on mouse samples at 1:1000 (fig 4a, 4c). Nat Commun (2021) ncbi
mouse monoclonal (A-4)
  • western blot; mouse; loading ...; fig 3d
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-365513) was used in western blot on mouse samples (fig 3d). Sci Rep (2020) ncbi
mouse monoclonal (2A4)
  • western blot; mouse; loading ...; fig 3d
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz Biotechnology (Santa Cruz, CA), sc-13551) was used in western blot on mouse samples (fig 3d). Pharmacol Res (2020) ncbi
mouse monoclonal (2A4)
  • western blot; mouse; loading ...; fig 1b, e1e
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-13551) was used in western blot on mouse samples (fig 1b, e1e). EMBO J (2019) ncbi
mouse monoclonal (2A4)
  • western blot; human; loading ...; fig 1c, s5c
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, 2A4) was used in western blot on human samples (fig 1c, s5c). Clin Sci (Lond) (2019) ncbi
mouse monoclonal (2A4)
  • western blot; human; loading ...; fig 6b
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-13551) was used in western blot on human samples (fig 6b). FEBS Lett (2018) ncbi
mouse monoclonal (2A4)
  • western blot; mouse; fig 2b
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-13551) was used in western blot on mouse samples (fig 2b). J Lipid Res (2016) ncbi
mouse monoclonal (2A4)
  • RNA immunoprecipitation; human; 1:50; fig 4
  • western blot; human; 1:1000; fig 5
  • western blot; mouse; 1:1000; fig 6
Santa Cruz Biotechnology Srebf1 antibody (santa Cruz, sc-13551) was used in RNA immunoprecipitation on human samples at 1:50 (fig 4), in western blot on human samples at 1:1000 (fig 5) and in western blot on mouse samples at 1:1000 (fig 6). Sci Rep (2016) ncbi
mouse monoclonal (A-4)
  • western blot; bovine; fig 1
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz Biotechnology, sc-365513) was used in western blot on bovine samples (fig 1). Int J Mol Sci (2015) ncbi
mouse monoclonal (A-4)
  • western blot; human; fig 6
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-365513) was used in western blot on human samples (fig 6). Cell Death Dis (2015) ncbi
mouse monoclonal (2A4)
  • western blot; human; loading ...; fig 4b
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, 2A4) was used in western blot on human samples (fig 4b). PLoS ONE (2015) ncbi
mouse monoclonal (2A4)
  • western blot; mouse; fig 7
In order to demonstrate that mTORC1 activation downregulates the CDK8-CycC complex and elevates lipogenic protein expression, Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, SC-13551) was used in western blot on mouse samples (fig 7). PLoS ONE (2015) ncbi
mouse monoclonal (2A4)
  • western blot; mouse; fig 2
  • western blot; human; fig 2
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, sc-13551) was used in western blot on mouse samples (fig 2) and in western blot on human samples (fig 2). Oncogene (2016) ncbi
mouse monoclonal (2A4)
  • western blot; human; fig s7
Santa Cruz Biotechnology Srebf1 antibody (santa Cruz, sc-13551) was used in western blot on human samples (fig s7). PLoS Genet (2015) ncbi
mouse monoclonal (2A4)
  • western blot; human; 1:500
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz Biotechnology, sc-13551) was used in western blot on human samples at 1:500. Cell Biochem Funct (2014) ncbi
mouse monoclonal (2A4)
  • western blot; human; loading ...; fig s2b
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, 2A4) was used in western blot on human samples (fig s2b). Mol Endocrinol (2014) ncbi
mouse monoclonal (2A4)
  • western blot; human
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz, 2A4) was used in western blot on human samples . J Biochem (2014) ncbi
mouse monoclonal (2A4)
  • western blot; human
Santa Cruz Biotechnology Srebf1 antibody (Santa Cruz Biotechnology, 2A4) was used in western blot on human samples . Proc Natl Acad Sci U S A (2005) ncbi
Novus Biologicals
mouse monoclonal (2A4)
  • western blot; mouse; 1:1000; loading ...; fig 6b
Novus Biologicals Srebf1 antibody (Novus Biologicals, NB #600-582) was used in western blot on mouse samples at 1:1000 (fig 6b). Commun Biol (2021) ncbi
mouse monoclonal (2A4)
  • western blot knockout validation; human; 1:1000; loading ...; fig 1c
Novus Biologicals Srebf1 antibody (Novus, NB600-582) was used in western blot knockout validation on human samples at 1:1000 (fig 1c). Front Pharmacol (2019) ncbi
mouse monoclonal (2A4)
  • western blot; human; loading ...; fig 1g
In order to demonstrate that RIDalpha utilizes ORP1L to fine-tune lipid raft cholesterol during adenovirus infection, Novus Biologicals Srebf1 antibody (Novus Biologicals, NB600-582) was used in western blot on human samples (fig 1g). J Virol (2017) ncbi
mouse monoclonal (2A4)
  • western blot; mouse
Novus Biologicals Srebf1 antibody (Novus Biologicals, NB600-582) was used in western blot on mouse samples . J Lipids (2013) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; rat; 1:200; loading ...; fig 5a
Abcam Srebf1 antibody (abcam, ab28481) was used in western blot on rat samples at 1:200 (fig 5a). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; fig s8c, s8d
  • western blot; mouse; loading ...; fig s8b
Abcam Srebf1 antibody (Abcam, ab28481) was used in western blot on human samples (fig s8c, s8d) and in western blot on mouse samples (fig s8b). Hepatology (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6a
Abcam Srebf1 antibody (Cell Signaling, ab28481) was used in western blot on mouse samples at 1:1000 (fig 6a). Int J Mol Med (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4c
Abcam Srebf1 antibody (Abcam, ab28481) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 4c). Int J Mol Med (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig 5c
Invitrogen Srebf1 antibody (Thermo Fisher Scientific, PA1-337) was used in western blot on rat samples at 1:1000 (fig 5c). Mol Med Rep (2021) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4f
Cell Signaling Technology Srebf1 antibody (CST, 9874) was used in western blot on human samples (fig 4f). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 8b
Cell Signaling Technology Srebf1 antibody (Cell Signaling, 9874) was used in western blot on human samples at 1:1000 (fig 8b). Toxicol Appl Pharmacol (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4f
Cell Signaling Technology Srebf1 antibody (CST, 9874S) was used in western blot on human samples (fig 4f). Nature (2017) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 8b
  • western blot; human; loading ...; fig 8a
In order to present the trophic and modulatory potential of statins in a dopaminergic cell line, MilliporeSigma Srebf1 antibody (Sigma-Aldrich, SAB4502850) was used in immunocytochemistry on human samples (fig 8b) and in western blot on human samples (fig 8a). Synapse (2016) ncbi
Articles Reviewed
  1. Han H, Kim S, Kim Y, Jang S, Kwon Y, Choi D, et al. A novel role of CRTC2 in promoting nonalcoholic fatty liver disease. Mol Metab. 2022;55:101402 pubmed publisher
  2. Zhao Y, Li W, Zhang D. Gycyrrhizic acid alleviates atherosclerotic lesions in rats with diabetes mellitus. Mol Med Rep. 2021;24: pubmed publisher
  3. Xu L, Zhang X, Xin Y, Ma J, Yang C, Zhang X, et al. Depdc5 deficiency exacerbates alcohol-induced hepatic steatosis via suppression of PPARα pathway. Cell Death Dis. 2021;12:710 pubmed publisher
  4. Li L, Yang L, Yang F, Zhao X, Xue S, Gong F. Ginkgo biloba Extract 50 (GBE50) Ameliorates Insulin Resistance, Hepatic Steatosis and Liver Injury in High Fat Diet-Fed Mice. J Inflamm Res. 2021;14:1959-1971 pubmed publisher
  5. Ma N, Wang Y, Xu S, Ni Q, Zheng Q, Zhu B, et al. PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling. Nat Commun. 2021;12:3059 pubmed publisher
  6. Luo L, Wu J, Lin T, Lian G, Wang H, Gao G, et al. Influence of atorvastatin on metabolic pattern of rats with pulmonary hypertension. Aging (Albany NY). 2021;13:11954-11968 pubmed publisher
  7. Zhang M, Ceyhan Y, Kaftanovskaya E, Vasquez J, Vacher J, Knop F, et al. INPP4B protects from metabolic syndrome and associated disorders. Commun Biol. 2021;4:416 pubmed publisher
  8. Park E, Yu T, Yang K, Choi S, Lee S, Park K. Cube natural sea salt ameliorates obesity in high fat diet-induced obese mice and 3T3-L1 adipocytes. Sci Rep. 2020;10:3407 pubmed publisher
  9. Aldonza M, Ku J, Hong J, Kim D, Yu S, Lee M, et al. Prior acquired resistance to paclitaxel relays diverse EGFR-targeted therapy persistence mechanisms. Sci Adv. 2020;6:eaav7416 pubmed publisher
  10. Yang S, Ma C, Wu H, Zhang H, Yuan F, Yang G, et al. Tectorigenin attenuates diabetic nephropathy by improving vascular endothelium dysfunction through activating AdipoR1/2 pathway. Pharmacol Res. 2020;153:104678 pubmed publisher
  11. Yang N, Li C, Li H, Liu M, Cai X, Cao F, et al. Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells. Front Pharmacol. 2019;10:709 pubmed publisher
  12. Su L, Zhou L, Chen F, Wang H, Qian H, Sheng Y, et al. Cideb controls sterol-regulated ER export of SREBP/SCAP by promoting cargo loading at ER exit sites. EMBO J. 2019;38: pubmed publisher
  13. Nakazeki F, Tsuge I, Horie T, Imamura K, Tsukita K, Hotta A, et al. MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons. Clin Sci (Lond). 2019;133:583-595 pubmed publisher
  14. Wang Y, Du L, Liang X, Meng P, Bi L, Wang Y, et al. Sirtuin 4 Depletion Promotes Hepatocellular Carcinoma Tumorigenesis Through Regulating Adenosine-Monophosphate-Activated Protein Kinase Alpha/Mammalian Target of Rapamycin Axis in Mice. Hepatology. 2018;: pubmed publisher
  15. Guo J, Fang W, Chen X, Lin Y, Hu G, Wei J, et al. Upstream stimulating factor 1 suppresses autophagy and hepatic lipid droplet catabolism by activating mTOR. FEBS Lett. 2018;592:2725-2738 pubmed publisher
  16. Song H, Li X, Liu Y, Lu W, Cui Z, Zhou L, et al. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS. Int J Mol Med. 2018;42:193-207 pubmed publisher
  17. Bai X, Hong W, Cai P, Chen Y, Xu C, Cao D, et al. Valproate induced hepatic steatosis by enhanced fatty acid uptake and triglyceride synthesis. Toxicol Appl Pharmacol. 2017;324:12-25 pubmed publisher
  18. Dey P, Baddour J, Muller F, Wu C, Wang H, Liao W, et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature. 2017;542:119-123 pubmed publisher
  19. Cianciola N, Chung S, Manor D, Carlin C. Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum. J Virol. 2017;91: pubmed publisher
  20. Kong Q, Zhang H, Zhao T, Zhang W, Yan M, Dong X, et al. Tangshen formula attenuates hepatic steatosis by inhibiting hepatic lipogenesis and augmenting fatty acid oxidation in db/db mice. Int J Mol Med. 2016;38:1715-1726 pubmed publisher
  21. Tong X, Li P, Zhang D, VanDommelen K, Gupta N, Rui L, et al. E4BP4 is an insulin-induced stabilizer of nuclear SREBP-1c and promotes SREBP-1c-mediated lipogenesis. J Lipid Res. 2016;57:1219-30 pubmed publisher
  22. Yan C, Chen J, Chen N. Long noncoding RNA MALAT1 promotes hepatic steatosis and insulin resistance by increasing nuclear SREBP-1c protein stability. Sci Rep. 2016;6:22640 pubmed publisher
  23. Schmitt M, Dehay B, Bezard E, Garcia Ladona F. Harnessing the trophic and modulatory potential of statins in a dopaminergic cell line. Synapse. 2016;70:71-86 pubmed publisher
  24. Ao J, Wei C, Si Y, Luo C, Lv W, Lin Y, et al. Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells. Int J Mol Sci. 2015;16:29936-47 pubmed publisher
  25. Laezza C, D Alessandro A, Di Croce L, Picardi P, Ciaglia E, Pisanti S, et al. p53 regulates the mevalonate pathway in human glioblastoma multiforme. Cell Death Dis. 2015;6:e1909 pubmed publisher
  26. Ochiai A, Miyata S, Shimizu M, Inoue J, Sato R. Piperine Induces Hepatic Low-Density Lipoprotein Receptor Expression through Proteolytic Activation of Sterol Regulatory Element-Binding Proteins. PLoS ONE. 2015;10:e0139799 pubmed publisher
  27. Feng D, Youn D, Zhao X, Gao Y, Quinn W, Xiaoli A, et al. mTORC1 Down-Regulates Cyclin-Dependent Kinase 8 (CDK8) and Cyclin C (CycC). PLoS ONE. 2015;10:e0126240 pubmed publisher
  28. Li S, Oh Y, Yue P, Khuri F, Sun S. Inhibition of mTOR complex 2 induces GSK3/FBXW7-dependent degradation of sterol regulatory element-binding protein 1 (SREBP1) and suppresses lipogenesis in cancer cells. Oncogene. 2016;35:642-50 pubmed publisher
  29. Kirschner K, Samarajiwa S, Cairns J, Menon S, Perez Mancera P, Tomimatsu K, et al. Phenotype specific analyses reveal distinct regulatory mechanism for chronically activated p53. PLoS Genet. 2015;11:e1005053 pubmed publisher
  30. Caputo M, De Rosa M, Rescigno T, Zirpoli H, Vassallo A, De Tommasi N, et al. Binding of polyunsaturated fatty acids to LXRα and modulation of SREBP-1 interaction with a specific SCD1 promoter element. Cell Biochem Funct. 2014;32:637-46 pubmed publisher
  31. Wang Y, Zhou D, Chen S. SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1. Mol Endocrinol. 2014;28:935-48 pubmed publisher
  32. Nakakuki M, Kawano H, Notsu T, Imada K, Mizuguchi K, Shimano H. A novel processing system of sterol regulatory element-binding protein-1c regulated by polyunsaturated fatty acid. J Biochem. 2014;155:301-13 pubmed publisher
  33. Wen S, Jadhav K, Williamson D, Rideout T. Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice. J Lipids. 2013;2013:908048 pubmed publisher
  34. Bengoechea Alonso M, Punga T, Ericsson J. Hyperphosphorylation regulates the activity of SREBP1 during mitosis. Proc Natl Acad Sci U S A. 2005;102:11681-6 pubmed