This is a Validated Antibody Database (VAD) review about rat Sptan1, based on 24 published articles (read how Labome selects the articles), using Sptan1 antibody in all methods. It is aimed to help Labome visitors find the most suited Sptan1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Sptan1 synonym: A2a; IPF; Spna2

Knockout validation
BioLegend
mouse monoclonal (D8B7)
  • immunocytochemistry knockout validation; mouse; loading ...; fig 2h, 2j
  • western blot knockout validation; mouse; loading ...; fig 2b
  • western blot; human; loading ...; fig 1a
BioLegend Sptan1 antibody (BioLegend, 803201) was used in immunocytochemistry knockout validation on mouse samples (fig 2h, 2j), in western blot knockout validation on mouse samples (fig 2b) and in western blot on human samples (fig 1a). J Biol Chem (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-3)
  • western blot; human; 1:500; fig 4c
Santa Cruz Biotechnology Sptan1 antibody (Santa Cruz Biotechnology, sc-48382) was used in western blot on human samples at 1:500 (fig 4c). Nat Commun (2021) ncbi
mouse monoclonal (C-11)
  • proximity ligation assay; mouse; loading ...; fig 11e
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 11a
Santa Cruz Biotechnology Sptan1 antibody (Santa Cruz, sc-46696) was used in proximity ligation assay on mouse samples (fig 11e) and in immunohistochemistry - paraffin section on mouse samples (fig 11a). Int J Mol Sci (2021) ncbi
mouse monoclonal (C-11)
  • western blot; mouse; 1:150; loading ...; fig 4a
Santa Cruz Biotechnology Sptan1 antibody (Santa Cruz Biotechnology, sc-46696) was used in western blot on mouse samples at 1:150 (fig 4a). J Clin Invest (2017) ncbi
mouse monoclonal (C-3)
  • western blot; rat; 1:200; fig 3
Santa Cruz Biotechnology Sptan1 antibody (Santa Cruz, sc-48382) was used in western blot on rat samples at 1:200 (fig 3). PLoS ONE (2016) ncbi
mouse monoclonal (C-11)
  • western blot; rat; fig 8
Santa Cruz Biotechnology Sptan1 antibody (Santa Cruz, sc-46696) was used in western blot on rat samples (fig 8). Evid Based Complement Alternat Med (2015) ncbi
mouse monoclonal (C-3)
  • western blot; mouse; 1:1000
Santa Cruz Biotechnology Sptan1 antibody (Santa Cruz Biotechnology, sc-48382) was used in western blot on mouse samples at 1:1000. J Neurosci Res (2015) ncbi
mouse monoclonal (C-3)
  • western blot; rat
Santa Cruz Biotechnology Sptan1 antibody (SCB, sc-48382) was used in western blot on rat samples . FASEB J (2014) ncbi
mouse monoclonal (C-3)
  • western blot; mouse; fig 1j
In order to demonstrate that TET proteins are degraded by calpain, Santa Cruz Biotechnology Sptan1 antibody (SantaCruz, sc-48382) was used in western blot on mouse samples (fig 1j). Cell Rep (2014) ncbi
mouse monoclonal (C-11)
  • immunohistochemistry - paraffin section; mouse; fig 1
Santa Cruz Biotechnology Sptan1 antibody (Santa Cruz Biotechnology, sc-46696) was used in immunohistochemistry - paraffin section on mouse samples (fig 1). Front Physiol (2013) ncbi
Abcam
mouse monoclonal (D8B7)
  • western blot; rat; 1:1000; loading ...; fig 3a
In order to study the neuroprotective effect of latanoprost, Abcam Sptan1 antibody (Abcam, ab11755) was used in western blot on rat samples at 1:1000 (fig 3a). J Neurochem (2017) ncbi
domestic rabbit monoclonal (EPR3830-43)
  • western blot; human; 1:10,000; loading ...; tbl s4
Abcam Sptan1 antibody (Abcam, ab133320) was used in western blot on human samples at 1:10,000 (tbl s4). Stem Cell Res (2016) ncbi
domestic rabbit monoclonal (EPR3017)
  • western blot; rat; 1:1000
Abcam Sptan1 antibody (Abcam, ab75755) was used in western blot on rat samples at 1:1000. J Neurochem (2014) ncbi
BioLegend
mouse monoclonal (D8B7)
  • immunocytochemistry knockout validation; mouse; loading ...; fig 2h, 2j
  • western blot knockout validation; mouse; loading ...; fig 2b
  • western blot; human; loading ...; fig 1a
BioLegend Sptan1 antibody (BioLegend, 803201) was used in immunocytochemistry knockout validation on mouse samples (fig 2h, 2j), in western blot knockout validation on mouse samples (fig 2b) and in western blot on human samples (fig 1a). J Biol Chem (2019) ncbi
mouse monoclonal (D8B7)
  • western blot; mouse; 1:250; loading ...; fig 2f
BioLegend Sptan1 antibody (BioLegend, 803201) was used in western blot on mouse samples at 1:250 (fig 2f). elife (2019) ncbi
BD Biosciences
mouse monoclonal (42/B-Spectrin II)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1d
BD Biosciences Sptan1 antibody (BD Transduction Lab, 612562) was used in immunohistochemistry on mouse samples at 1:200 (fig 1d). Nat Commun (2021) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunocytochemistry; human; 1:200; loading ...; fig 4f
  • immunocytochemistry; rat; 1:200; loading ...; fig 1e
BD Biosciences Sptan1 antibody (BD Transduction, 612563) was used in immunocytochemistry on human samples at 1:200 (fig 4f) and in immunocytochemistry on rat samples at 1:200 (fig 1e). elife (2020) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5d
BD Biosciences Sptan1 antibody (BD Biosciences, 612563) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 5d). elife (2020) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunohistochemistry; mouse; fig 4b
BD Biosciences Sptan1 antibody (BD Bioscience, 612562) was used in immunohistochemistry on mouse samples (fig 4b). EMBO Rep (2017) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunohistochemistry; chicken; 1:500; loading ...; fig 9a
  • immunohistochemistry; mouse; 1:500; loading ...; fig 2c
In order to elucidate that Emx2 expression is required to reverse hair bundles from their 'default' polarity in the macula and neuromast, BD Biosciences Sptan1 antibody (BD Biosciences, 612562) was used in immunohistochemistry on chicken samples at 1:500 (fig 9a) and in immunohistochemistry on mouse samples at 1:500 (fig 2c). elife (2017) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunocytochemistry; mouse; loading ...; fig s6a
BD Biosciences Sptan1 antibody (BD Biosciences, 612563) was used in immunocytochemistry on mouse samples (fig s6a). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunocytochemistry; rat; 1:200; fig 2
  • immunocytochemistry; mouse; 1:200; fig 1
BD Biosciences Sptan1 antibody (BD Biosciences, 612563) was used in immunocytochemistry on rat samples at 1:200 (fig 2) and in immunocytochemistry on mouse samples at 1:200 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunocytochemistry; rat; fig 7
BD Biosciences Sptan1 antibody (BD Biosciences, 612563) was used in immunocytochemistry on rat samples (fig 7). elife (2014) ncbi
mouse monoclonal (42/B-Spectrin II)
  • immunohistochemistry; rat; 1:400
BD Biosciences Sptan1 antibody (BD Biosciences, 612562) was used in immunohistochemistry on rat samples at 1:400. Hum Mol Genet (2015) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (3H1)
  • western blot; fruit fly ; 1:500; loading ...; fig 3c
Developmental Studies Hybridoma Bank Sptan1 antibody (DSHB, 3H1) was used in western blot on fruit fly samples at 1:500 (fig 3c). J Cell Biol (2017) ncbi
Articles Reviewed
  1. Hu D, Sun X, Magpusao A, Fedorov Y, Thompson M, Wang B, et al. Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. Nat Commun. 2021;12:5305 pubmed publisher
  2. Kindt K, Akturk A, Jarysta A, Day M, Beirl A, Flonard M, et al. EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia. Nat Commun. 2021;12:2861 pubmed publisher
  3. Baasch Christensen I, Cheng L, Brewer J, Bartsch U, Fenton R, H Damkier H, et al. Multiple Na,K-ATPase Subunits Colocalize in the Brush Border of Mouse Choroid Plexus Epithelial Cells. Int J Mol Sci. 2021;22: pubmed publisher
  4. Costa A, Sousa S, Pinto Costa R, Mateus J, Lopes C, Costa A, et al. The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction. elife. 2020;9: pubmed publisher
  5. Liu C, Stevens S, Teliska L, Stankewich M, Mohler P, Hund T, et al. Nodal β spectrins are required to maintain Na+ channel clustering and axon integrity. elife. 2020;9: pubmed publisher
  6. Lubbers E, Murphy N, Musa H, Huang C, Gupta R, Price M, et al. Defining new mechanistic roles for αII spectrin in cardiac function. J Biol Chem. 2019;294:9576-9591 pubmed publisher
  7. Wang G, Simon D, Wu Z, Belsky D, Heller E, O Rourke M, et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. elife. 2019;8: pubmed publisher
  8. Kumar A, Dejanovic B, Hetsch F, Semtner M, Fusca D, Arjune S, et al. S-sulfocysteine/NMDA receptor-dependent signaling underlies neurodegeneration in molybdenum cofactor deficiency. J Clin Invest. 2017;127:4365-4378 pubmed publisher
  9. Vogl C, Butola T, Haag N, Hausrat T, Leitner M, Moutschen M, et al. The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearing. EMBO Rep. 2017;18:2015-2029 pubmed publisher
  10. Földi I, Anthoney N, Harrison N, Gangloff M, Verstak B, Nallasivan M, et al. Three-tier regulation of cell number plasticity by neurotrophins and Tolls in Drosophila. J Cell Biol. 2017;216:1421-1438 pubmed publisher
  11. Jiang T, Kindt K, Wu D. Transcription factor Emx2 controls stereociliary bundle orientation of sensory hair cells. elife. 2017;6: pubmed publisher
  12. Yamamoto K, Sato K, Yukita M, Yasuda M, Omodaka K, Ryu M, et al. The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection. J Neurochem. 2017;140:495-508 pubmed publisher
  13. Hansen S, Stummann T, Borland H, Hasholt L, Tumer Z, Nielsen J, et al. Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3. Stem Cell Res. 2016;17:306-317 pubmed publisher
  14. He J, Zhou R, Wu Z, Carrasco M, Kurshan P, Farley J, et al. Prevalent presence of periodic actin-spectrin-based membrane skeleton in a broad range of neuronal cell types and animal species. Proc Natl Acad Sci U S A. 2016;113:6029-34 pubmed publisher
  15. D Este E, Kamin D, Velte C, Göttfert F, Simons M, Hell S. Subcortical cytoskeleton periodicity throughout the nervous system. Sci Rep. 2016;6:22741 pubmed publisher
  16. Talbert E, Smuder A, Kwon O, Sollanek K, Wiggs M, Powers S. Blockage of the Ryanodine Receptor via Azumolene Does Not Prevent Mechanical Ventilation-Induced Diaphragm Atrophy. PLoS ONE. 2016;11:e0148161 pubmed publisher
  17. Guo C, Zheng L, Fu J, Zhu J, Zhou Y, Zeng T, et al. Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism. Evid Based Complement Alternat Med. 2015;2015:514063 pubmed publisher
  18. Zhong G, He J, Zhou R, LORENZO D, Babcock H, Bennett V, et al. Developmental mechanism of the periodic membrane skeleton in axons. elife. 2014;3: pubmed publisher
  19. Requena T, Cabrera S, Martín Sierra C, Price S, Lysakowski A, Lopez Escamez J. Identification of two novel mutations in FAM136A and DTNA genes in autosomal-dominant familial Meniere's disease. Hum Mol Genet. 2015;24:1119-26 pubmed publisher
  20. Singhal K, Sandhir R. L-type calcium channel blocker ameliorates diabetic encephalopathy by modulating dysregulated calcium homeostasis. J Neurosci Res. 2015;93:296-308 pubmed publisher
  21. Smith I, Godinez G, Singh B, McCaughey K, Alcantara R, Gururaja T, et al. Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction. FASEB J. 2014;28:2790-803 pubmed publisher
  22. Campos Martorell M, Salvador N, Monge M, Canals F, Garcia Bonilla L, Hernandez Guillamon M, et al. Brain proteomics identifies potential simvastatin targets in acute phase of stroke in a rat embolic model. J Neurochem. 2014;130:301-12 pubmed publisher
  23. Wang Y, Zhang Y. Regulation of TET protein stability by calpains. Cell Rep. 2014;6:278-84 pubmed publisher
  24. Christensen I, Gyldenholm T, Damkier H, Praetorius J. Polarization of membrane associated proteins in the choroid plexus epithelium from normal and slc4a10 knockout mice. Front Physiol. 2013;4:344 pubmed publisher