This is a Validated Antibody Database (VAD) review about rat Spp1, based on 41 published articles (read how Labome selects the articles), using Spp1 antibody in all methods. It is aimed to help Labome visitors find the most suited Spp1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Spp1 synonym: OSP

Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 6b
Abcam Spp1 antibody (Abcam, ab8448) was used in western blot knockout validation on human samples (fig 6b). elife (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (AKm2A1)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 4c
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in immunohistochemistry on mouse samples at 1:100 (fig 4c). FEBS Open Bio (2020) ncbi
mouse monoclonal (LFMb-14)
  • western blot; mouse; 1:500; loading ...; fig 6a
Santa Cruz Biotechnology Spp1 antibody (Santa, sc-73631) was used in western blot on mouse samples at 1:500 (fig 6a). Front Immunol (2019) ncbi
mouse monoclonal (LFMb-14)
  • western blot; human; 1:200; loading ...; fig 5g
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-73631) was used in western blot on human samples at 1:200 (fig 5g). Int J Biol Sci (2017) ncbi
mouse monoclonal (AKm2A1)
  • immunocytochemistry; rat; fig 2c
  • western blot; rat; fig 3b
  • western blot; mouse; loading ...; fig 7d
In order to determine the mechanism by which tiron functions, Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in immunocytochemistry on rat samples (fig 2c), in western blot on rat samples (fig 3b) and in western blot on mouse samples (fig 7d). Clin Exp Pharmacol Physiol (2017) ncbi
mouse monoclonal (AKm2A1)
  • western blot; rat; loading ...; fig 8a
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in western blot on rat samples (fig 8a). PLoS ONE (2017) ncbi
mouse monoclonal (AKm2A1)
  • immunohistochemistry - paraffin section; mouse; fig 5h
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc21742) was used in immunohistochemistry - paraffin section on mouse samples (fig 5h). Hum Mol Genet (2017) ncbi
mouse monoclonal (LFMb-14)
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 2
  • western blot; human; 1:2500; loading ...; fig 3a
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-73631) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 2) and in western blot on human samples at 1:2500 (fig 3a). Oncotarget (2016) ncbi
mouse monoclonal (AKm2A1)
  • immunoprecipitation; mouse; fig 4
  • western blot; mouse; fig 1
Santa Cruz Biotechnology Spp1 antibody (santa Cruz, sc-21742) was used in immunoprecipitation on mouse samples (fig 4) and in western blot on mouse samples (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (AKm2A1)
  • western blot; mouse; loading ...; fig 8b
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in western blot on mouse samples (fig 8b). PLoS ONE (2016) ncbi
mouse monoclonal (LFMb-14)
  • western blot; human; loading ...; fig 7c
Santa Cruz Biotechnology Spp1 antibody (SantaCruz, sc-73631) was used in western blot on human samples (fig 7c). Oncogene (2016) ncbi
mouse monoclonal (AKm2A1)
  • immunocytochemistry; rat; fig 5
  • western blot; rat; fig 2
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, SC-21742) was used in immunocytochemistry on rat samples (fig 5) and in western blot on rat samples (fig 2). Int J Mol Sci (2015) ncbi
mouse monoclonal (AKm2A1)
  • western blot; rat; 1:1000; fig 8
Santa Cruz Biotechnology Spp1 antibody (santa Cruz, sc-21742) was used in western blot on rat samples at 1:1000 (fig 8). Mol Med Rep (2015) ncbi
mouse monoclonal (AKm2A1)
  • western blot; mouse
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz Biotechnology, sc-21742) was used in western blot on mouse samples . J Mol Cell Cardiol (2015) ncbi
mouse monoclonal (AKm2A1)
  • western blot; mouse; fig 5
Santa Cruz Biotechnology Spp1 antibody (santa cruz, sc-21742) was used in western blot on mouse samples (fig 5). Int J Mol Med (2015) ncbi
mouse monoclonal (AKm2A1)
  • immunocytochemistry; mouse; 1:100; fig 4
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in immunocytochemistry on mouse samples at 1:100 (fig 4). PLoS ONE (2015) ncbi
mouse monoclonal (AKm2A1)
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 7
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz Biotechnology, sc-21742) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 7). Mol Med Rep (2015) ncbi
mouse monoclonal (AKm2A1)
  • immunocytochemistry; human; 1:100; tbl 1
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc21742) was used in immunocytochemistry on human samples at 1:100 (tbl 1). Acta Biomater (2015) ncbi
mouse monoclonal (AKm2A1)
  • western blot; rat
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in western blot on rat samples . Eur J Pharmacol (2014) ncbi
mouse monoclonal (AKm2A1)
  • immunocytochemistry; mouse
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in immunocytochemistry on mouse samples . Proc Natl Acad Sci U S A (2014) ncbi
mouse monoclonal (AKm2A1)
  • western blot; rat; 1:500
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, SC-21742) was used in western blot on rat samples at 1:500. Urology (2014) ncbi
mouse monoclonal (AKm2A1)
  • immunocytochemistry; human; 2 ug/ml
Santa Cruz Biotechnology Spp1 antibody (Santa Cruz, sc-21742) was used in immunocytochemistry on human samples at 2 ug/ml. J Tissue Eng Regen Med (2015) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig 2e
Abcam Spp1 antibody (Abcam, ab8448) was used in western blot on rat samples at 1:1000 (fig 2e). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s1b
Abcam Spp1 antibody (abcam, ab8448) was used in western blot on human samples (fig s1b). BMC Cancer (2021) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 6b
Abcam Spp1 antibody (Abcam, ab8448) was used in western blot knockout validation on human samples (fig 6b). elife (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig 2a
Abcam Spp1 antibody (Abcam, ab8448) was used in immunocytochemistry on human samples at 1:1000 (fig 2a). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; rat; 1:500; loading ...; fig 2a
Abcam Spp1 antibody (Abcam, ab8448) was used in immunohistochemistry on rat samples at 1:500 (fig 2a). Front Cell Neurosci (2019) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 4b
Abcam Spp1 antibody (Abcam, ab8448) was used in western blot on rat samples (fig 4b). Biosci Rep (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a
Abcam Spp1 antibody (Abcam, ab8448) was used in western blot on human samples at 1:1000 (fig 1a). Biomed Pharmacother (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:200; loading ...; fig 5h
Abcam Spp1 antibody (Abcam, ab8448) was used in immunohistochemistry - frozen section on human samples at 1:200 (fig 5h). J Histochem Cytochem (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1f
  • western blot; human; fig 1b
Abcam Spp1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1f) and in western blot on human samples (fig 1b). PLoS ONE (2017) ncbi
domestic rabbit polyclonal
  • flow cytometry; rat; loading ...; fig 7c
  • western blot; rat; loading ...; fig 3c
Abcam Spp1 antibody (Abcam, ab8448) was used in flow cytometry on rat samples (fig 7c) and in western blot on rat samples (fig 3c). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; human; fig 6
Abcam Spp1 antibody (Abcam, ab8448) was used in immunohistochemistry - frozen section on human samples (fig 6). Front Physiol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000
In order to study the role of kif3a in dental mesenchymal stem and precursor cell differentiation, Abcam Spp1 antibody (Abcam, ab8448) was used in western blot on human samples at 1:1000. Mol Med Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:500; fig 5
Abcam Spp1 antibody (abcam, ab8448) was used in western blot on mouse samples at 1:500 (fig 5). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 5b
In order to analyze NFATc1 and its promotion of prostate tumorigenesis and its ability to overcome PTEN loss-induced senescence, Abcam Spp1 antibody (Abcam, ab8448) was used in immunohistochemistry on mouse samples at 1:100 (fig 5b). Oncogene (2016) ncbi
Novus Biologicals
mouse monoclonal (1B20)
  • immunohistochemistry - paraffin section; domestic rabbit; loading ...; fig 7b
Novus Biologicals Spp1 antibody (Novus Biologicals, 1B20) was used in immunohistochemistry - paraffin section on domestic rabbit samples (fig 7b). Sci Rep (2016) ncbi
mouse monoclonal (1B20)
  • immunohistochemistry - paraffin section; domestic rabbit; 1:100
  • immunocytochemistry; domestic rabbit; 1:200
Novus Biologicals Spp1 antibody (Novus Biologicals, NB110-89062) was used in immunohistochemistry - paraffin section on domestic rabbit samples at 1:100 and in immunocytochemistry on domestic rabbit samples at 1:200. Tissue Eng Part A (2014) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (MPIIIB10(1))
  • immunohistochemistry; chicken; fig 4
Developmental Studies Hybridoma Bank Spp1 antibody (Developmental Studies Hybridoma Bank, MPIIIB10) was used in immunohistochemistry on chicken samples (fig 4). Front Neuroanat (2016) ncbi
mouse monoclonal (MPIIIB10(1))
  • immunocytochemistry; mouse; 1:2000; fig 2
  • western blot; mouse; 1:50,000; fig 5f
In order to characterize intramembranous and endochondral ossification peroxisomes in different skeletal cell types and regulation of osteoblast differentiation by distinct peroxisome proliferator-activated receptors, Developmental Studies Hybridoma Bank Spp1 antibody (Developmental Studies Hybridoma Bank (DSHB), MPIIIB101) was used in immunocytochemistry on mouse samples at 1:2000 (fig 2) and in western blot on mouse samples at 1:50,000 (fig 5f). PLoS ONE (2015) ncbi
mouse monoclonal (MPIIIB10(1))
  • immunohistochemistry - paraffin section; rat; 1:300; fig 3
Developmental Studies Hybridoma Bank Spp1 antibody (Developmental Hybridoma Studies Institute, MPIIIB10) was used in immunohistochemistry - paraffin section on rat samples at 1:300 (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (MPIIIB10(1))
  • immunocytochemistry; human; 1:5
Developmental Studies Hybridoma Bank Spp1 antibody (DSHB, MPIIIB10) was used in immunocytochemistry on human samples at 1:5. Anticancer Res (2004) ncbi
Articles Reviewed
  1. Han H, Tian T, Huang G, Li D, Yang S. The lncRNA H19/miR-541-3p/Wnt/β-catenin axis plays a vital role in melatonin-mediated osteogenic differentiation of bone marrow mesenchymal stem cells. Aging (Albany NY). 2021;13:18257-18273 pubmed publisher
  2. Huang J, Hu M, Niu H, Wang J, Si Y, Cheng S, et al. Osteopontin isoform c promotes the survival of cisplatin-treated NSCLC cells involving NFATc2-mediated suppression on calcium-induced ROS levels. BMC Cancer. 2021;21:750 pubmed publisher
  3. Hendley A, Rao A, Leonhardt L, Ashe S, Smith J, Giacometti S, et al. Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree. elife. 2021;10: pubmed publisher
  4. Barney L, Hall C, Schwartz A, Parks A, Sparages C, Galarza S, et al. Tumor cell-organized fibronectin maintenance of a dormant breast cancer population. Sci Adv. 2020;6:eaaz4157 pubmed publisher
  5. Hirano Y, Kurosu H, Shiizaki K, Iwazu Y, Tsuruoka S, Kuro O M. Interleukin-36α as a potential biomarker for renal tubular damage induced by dietary phosphate load. FEBS Open Bio. 2020;10:894-903 pubmed publisher
  6. Wepler M, Preuss J, Merz T, Hartmann C, Wachter U, McCook O, et al. Impaired Glucocorticoid Receptor Dimerization Aggravates LPS-Induced Circulatory and Pulmonary Dysfunction. Front Immunol. 2019;10:3152 pubmed publisher
  7. Rabenstein M, Unverricht Yeboah M, Keuters M, Pikhovych A, Hucklenbroich J, Vay S, et al. Transcranial Current Stimulation Alters the Expression of Immune-Mediating Genes. Front Cell Neurosci. 2019;13:461 pubmed publisher
  8. Fang G, Qi J, Huang L, Zhao X. LncRNA MRAK048635_P1 is critical for vascular smooth muscle cell function and phenotypic switching in essential hypertension. Biosci Rep. 2019;: pubmed publisher
  9. He S, Nian F, Chen W, Yin L, Auchoybur M, Tao Z, et al. I-κB kinase-ε knockout protects against angiotensin II induced aortic valve thickening in apolipoprotein E deficient mice. Biomed Pharmacother. 2019;109:1287-1295 pubmed publisher
  10. Hira V, Wormer J, Kakar H, Breznik B, van der Swaan B, Hulsbos R, et al. Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins. J Histochem Cytochem. 2018;66:155-173 pubmed publisher
  11. Wong J, Wei R, Lyu P, Tong O, Zhang S, Wen Q, et al. Clinical and in vitro analysis of Osteopontin as a prognostic indicator and unveil its potential downstream targets in bladder cancer. Int J Biol Sci. 2017;13:1373-1386 pubmed publisher
  12. Dickerson M, Vierra N, Milian S, Dadi P, Jacobson D. Osteopontin activates the diabetes-associated potassium channel TALK-1 in pancreatic β-cells. PLoS ONE. 2017;12:e0175069 pubmed publisher
  13. Jiang P, Zhang D, Qiu H, Yi X, Zhang Y, Cao Y, et al. Tiron ameliorates high glucose-induced cardiac myocyte apoptosis by PKCδ-dependent inhibition of osteopontin. Clin Exp Pharmacol Physiol. 2017;44:760-770 pubmed publisher
  14. Jang M, Lee S, Baek S, Park S, Choi Y, Kim C. α-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS ONE. 2017;12:e0170699 pubmed publisher
  15. Jin F, Jiang K, Ji S, Wang L, Ni Z, Huang F, et al. Deficient TSC1/TSC2-complex suppression of SOX9-osteopontin-AKT signalling cascade constrains tumour growth in tuberous sclerosis complex. Hum Mol Genet. 2017;26:407-419 pubmed publisher
  16. Feng W, Zhang K, Liu Y, Chen J, Cai Q, Zhang Y, et al. Apocynin attenuates angiotensin II-induced vascular smooth muscle cells osteogenic switching via suppressing extracellular signal-regulated kinase 1/2. Oncotarget. 2016;7:83588-83600 pubmed publisher
  17. Stanic K, Saldivia N, Förstera B, Torrejon M, Montecinos H, Caprile T. Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development. Front Neuroanat. 2016;10:89 pubmed
  18. Spina A, Montella R, Liccardo D, De Rosa A, Laino L, Mitsiadis T, et al. NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression. Front Physiol. 2016;7:354 pubmed publisher
  19. Jiang S, Chen G, Feng L, Jiang Z, Yu M, Bao J, et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep. 2016;14:1891-900 pubmed publisher
  20. Anunobi C, Koli K, Saxena G, Banjo A, Ogbureke K. Expression of the SIBLINGs and their MMP partners in human benign and malignant prostate neoplasms. Oncotarget. 2016;7:48038-48049 pubmed publisher
  21. Cheng M, Wahafu T, Jiang G, Liu W, Qiao Y, Peng X, et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep. 2016;6:24134 pubmed publisher
  22. Yan B, Zhang Z, Jin D, Cai C, Jia C, Liu W, et al. mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation. Nat Commun. 2016;7:11151 pubmed publisher
  23. Zhao K, Zhang M, Zhang L, Wang P, Song G, Liu B, et al. Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response. Sci Rep. 2016;6:23771 pubmed publisher
  24. Calyjur P, Almeida C, Ayub Guerrieri D, Ribeiro A, Fernandes S, Ishiba R, et al. The mdx Mutation in the 129/Sv Background Results in a Milder Phenotype: Transcriptome Comparative Analysis Searching for the Protective Factors. PLoS ONE. 2016;11:e0150748 pubmed publisher
  25. Ying M, Zhang L, Zhou Q, Shao X, Cao J, Zhang N, et al. The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα. Oncogene. 2016;35:4358-67 pubmed publisher
  26. Qian G, Fan W, Ahlemeyer B, Karnati S, Baumgart Vogt E. Peroxisomes in Different Skeletal Cell Types during Intramembranous and Endochondral Ossification and Their Regulation during Osteoblast Differentiation by Distinct Peroxisome Proliferator-Activated Receptors. PLoS ONE. 2015;10:e0143439 pubmed publisher
  27. Manda K, Tripathi P, Hsi A, Ning J, Ruzinova M, Liapis H, et al. NFATc1 promotes prostate tumorigenesis and overcomes PTEN loss-induced senescence. Oncogene. 2016;35:3282-92 pubmed publisher
  28. He D, Lu Y, Hu H, Zhang J, Qin B, Wang Y, et al. The Wnt11 Signaling Pathway in Potential Cellular EMT and Osteochondral Differentiation Progression in Nephrolithiasis Formation. Int J Mol Sci. 2015;16:16313-29 pubmed publisher
  29. Sun J, Li J, Li C, Yu Y. Role of bone morphogenetic protein-2 in osteogenic differentiation of mesenchymal stem cells. Mol Med Rep. 2015;12:4230-4237 pubmed publisher
  30. Frenay A, Yazdani S, Boersema M, van der Graaf A, Waanders F, van den Born J, et al. Incomplete Restoration of Angiotensin II-Induced Renal Extracellular Matrix Deposition and Inflammation Despite Complete Functional Recovery in Rats. PLoS ONE. 2015;10:e0129732 pubmed publisher
  31. Seo K, Lee S, Ye B, Kim Y, Bae S, Kim C. Mechanical stretch enhances the expression and activity of osteopontin and MMP-2 via the Akt1/AP-1 pathways in VSMC. J Mol Cell Cardiol. 2015;85:13-24 pubmed publisher
  32. Li L, Dong Q, Wang Y, Feng Q, Zhou P, Ou X, et al. Hedgehog signaling is involved in the BMP9-induced osteogenic differentiation of mesenchymal stem cells. Int J Mol Med. 2015;35:1641-50 pubmed publisher
  33. Bouet G, Bouleftour W, Juignet L, Linossier M, Thomas M, Vanden Bossche A, et al. The impairment of osteogenesis in bone sialoprotein (BSP) knockout calvaria cell cultures is cell density dependent. PLoS ONE. 2015;10:e0117402 pubmed publisher
  34. Harada K, Harada T, Ferdous T, Takenawa T, Ueyama Y. Osteogenic cell fractions isolated from mouse tongue muscle. Mol Med Rep. 2015;12:31-6 pubmed publisher
  35. Johnstone S, Liley M, Dalby M, Barnett S. Comparison of human olfactory and skeletal MSCs using osteogenic nanotopography to demonstrate bone-specific bioactivity of the surfaces. Acta Biomater. 2015;13:266-76 pubmed publisher
  36. Ndisang J, Chibbar R, Lane N. Heme oxygenase suppresses markers of heart failure and ameliorates cardiomyopathy in L-NAME-induced hypertension. Eur J Pharmacol. 2014;734:23-34 pubmed publisher
  37. Inoue M, Arikawa T, Chen Y, Moriwaki Y, Price M, Brown M, et al. T cells down-regulate macrophage TNF production by IRAK1-mediated IL-10 expression and control innate hyperinflammation. Proc Natl Acad Sci U S A. 2014;111:5295-300 pubmed publisher
  38. Nguyen T, Lee B. A combination of biphasic calcium phosphate scaffold with hyaluronic acid-gelatin hydrogel as a new tool for bone regeneration. Tissue Eng Part A. 2014;20:1993-2004 pubmed publisher
  39. Jia Z, Wang S, Tang J, He D, Cui L, Liu Z, et al. Does crystal deposition in genetic hypercalciuric rat kidney tissue share similarities with bone formation?. Urology. 2014;83:509.e7-14 pubmed publisher
  40. Kaivosoja E, Sariola V, Chen Y, Konttinen Y. The effect of pulsed electromagnetic fields and dehydroepiandrosterone on viability and osteo-induction of human mesenchymal stem cells. J Tissue Eng Regen Med. 2015;9:31-40 pubmed publisher
  41. Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed