This is a Validated Antibody Database (VAD) review about rat Sirt6, based on 15 published articles (read how Labome selects the articles), using Sirt6 antibody in all methods. It is aimed to help Labome visitors find the most suited Sirt6 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 1s1j
Abcam Sirt6 antibody (abcam, ab62739) was used in western blot knockout validation on human samples at 1:1000 (fig 1s1j). elife (2020) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D8D12)
  • western blot knockout validation; mouse; loading ...; fig 4a
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, 12486) was used in western blot knockout validation on mouse samples (fig 4a) and in western blot on human samples (fig 2c). elife (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D8D12)
  • immunocytochemistry knockout validation; human; 1:100; fig 1
  • western blot knockout validation; human; 1:1000; fig 1
  • immunoprecipitation; human; 1:1000; fig s5
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, 12486) was used in immunocytochemistry knockout validation on human samples at 1:100 (fig 1), in western blot knockout validation on human samples at 1:1000 (fig 1) and in immunoprecipitation on human samples at 1:1000 (fig s5). Cell Res (2016) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 2a
  • western blot; human; 1:2000; loading ...; fig 3a
Abcam Sirt6 antibody (Abcam, ab62739) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2a) and in western blot on human samples at 1:2000 (fig 3a). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1b
  • chromatin immunoprecipitation; human; loading ...; fig 3e
  • western blot; human; loading ...; fig 2c
Abcam Sirt6 antibody (Abcam, ab62739) was used in western blot on mouse samples (fig 1b), in chromatin immunoprecipitation on human samples (fig 3e) and in western blot on human samples (fig 2c). Aging (Albany NY) (2020) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 1s1j
Abcam Sirt6 antibody (abcam, ab62739) was used in western blot knockout validation on human samples at 1:1000 (fig 1s1j). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5a
Abcam Sirt6 antibody (Abcam, ab62739) was used in western blot on mouse samples (fig 5a). Cell (2019) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 7
  • western blot; mouse; 1:1000; fig 1
Abcam Sirt6 antibody (Abcam, ab62739) was used in chromatin immunoprecipitation on mouse samples (fig 7) and in western blot on mouse samples at 1:1000 (fig 1). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D8D12)
  • western blot; human; 1:1000; loading ...
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, 12486) was used in western blot on human samples at 1:1000. Sci Adv (2021) ncbi
domestic rabbit monoclonal (D8D12)
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig e1d
  • immunohistochemistry; human; 1:100; fig e7e
  • western blot; human; fig e6a
Cell Signaling Technology Sirt6 antibody (Cell Signaling, 12486) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig e1d), in immunohistochemistry on human samples at 1:100 (fig e7e) and in western blot on human samples (fig e6a). Nat Metab (2021) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot; mouse; loading ...; fig 1b
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology Sirt6 antibody (Cell Signaling, 12486) was used in western blot on mouse samples (fig 1b) and in western blot on human samples (fig 2c). Aging (Albany NY) (2020) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot; human; 1:1000; loading ...; fig 2e
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1a
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1b
  • western blot; mouse; 1:1000; loading ...; fig 1a, 1b
Cell Signaling Technology Sirt6 antibody (Cell Signaling, 12486S) was used in western blot on human samples at 1:1000 (fig 2e), in immunocytochemistry on mouse samples at 1:200 (fig 1a), in immunohistochemistry on mouse samples at 1:200 (fig 1b) and in western blot on mouse samples at 1:1000 (fig 1a, 1b). Hepatology (2021) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot; mouse; loading ...; fig 4d
  • western blot; human; loading ...; fig 2d
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, D8D12) was used in western blot on mouse samples (fig 4d) and in western blot on human samples (fig 2d). Cell Rep (2019) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot; mouse; 1:1000; loading ...; fig s10
Cell Signaling Technology Sirt6 antibody (Cell Signaling, 12486) was used in western blot on mouse samples at 1:1000 (fig s10). Bone Res (2018) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot; mouse; 1:500; loading ...; fig s3s5
Cell Signaling Technology Sirt6 antibody (Cell Signaling, 12486) was used in western blot on mouse samples at 1:500 (fig s3s5). elife (2018) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot knockout validation; mouse; loading ...; fig 4a
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, 12486) was used in western blot knockout validation on mouse samples (fig 4a) and in western blot on human samples (fig 2c). elife (2017) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot; human; loading ...; fig 8b
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, D8D12) was used in western blot on human samples (fig 8b). Sci Rep (2016) ncbi
domestic rabbit monoclonal (D8D12)
  • immunocytochemistry knockout validation; human; 1:100; fig 1
  • western blot knockout validation; human; 1:1000; fig 1
  • immunoprecipitation; human; 1:1000; fig s5
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, 12486) was used in immunocytochemistry knockout validation on human samples at 1:100 (fig 1), in western blot knockout validation on human samples at 1:1000 (fig 1) and in immunoprecipitation on human samples at 1:1000 (fig s5). Cell Res (2016) ncbi
domestic rabbit monoclonal (D8D12)
  • western blot; human
Cell Signaling Technology Sirt6 antibody (Cell Signaling Technology, 12486) was used in western blot on human samples . PLoS ONE (2014) ncbi
Articles Reviewed
  1. Shi X, Jiang Y, Kitano A, Hu T, Murdaugh R, Li Y, et al. Nuclear NAD+ homeostasis governed by NMNAT1 prevents apoptosis of acute myeloid leukemia stem cells. Sci Adv. 2021;7: pubmed publisher
  2. Choi J, Sebastian C, Ferrer C, Lewis C, Sade Feldman M, LaSalle T, et al. A unique subset of glycolytic tumour-propagating cells drives squamous cell carcinoma. Nat Metab. 2021;3:182-195 pubmed publisher
  3. Chen J, Liu Z, Wang H, Qian L, Li Z, Song Q, et al. SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients. Aging (Albany NY). 2021;13:6025-6040 pubmed publisher
  4. Rezazadeh S, Yang D, Biashad S, Firsanov D, Takasugi M, Gilbert M, et al. SIRT6 mono-ADP ribosylates KDM2A to locally increase H3K36me2 at DNA damage sites to inhibit transcription and promote repair. Aging (Albany NY). 2020;12:11165-11184 pubmed publisher
  5. Zhang J, Li Y, Liu Q, Huang Y, Li R, Wu T, et al. Sirt6 Alleviated Liver Fibrosis by Deacetylating Conserved Lysine 54 on Smad2 in Hepatic Stellate Cells. Hepatology. 2021;73:1140-1157 pubmed publisher
  6. Onn L, Portillo M, Ilic S, Cleitman G, Stein D, Kaluski S, et al. SIRT6 is a DNA double-strand break sensor. elife. 2020;9: pubmed publisher
  7. Naiman S, Huynh F, Gil R, Glick Y, Shahar Y, Touitou N, et al. SIRT6 Promotes Hepatic Beta-Oxidation via Activation of PPARĪ±. Cell Rep. 2019;29:4127-4143.e8 pubmed publisher
  8. Tian X, Firsanov D, Zhang Z, Cheng Y, Luo L, Tombline G, et al. SIRT6 Is Responsible for More Efficient DNA Double-Strand Break Repair in Long-Lived Species. Cell. 2019;177:622-638.e22 pubmed publisher
  9. Chen X, Zhi X, Wang J, Su J. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res. 2018;6:34 pubmed publisher
  10. Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
  11. Zhang X, Spiegelman N, Nelson O, Jing H, Lin H. SIRT6 regulates Ras-related protein R-Ras2 by lysine defatty-acylation. elife. 2017;6: pubmed publisher
  12. Jiang H, Zhang X, Lin H. Lysine fatty acylation promotes lysosomal targeting of TNF-?. Sci Rep. 2016;6:24371 pubmed publisher
  13. Zhang Z, Ren S, Tan Y, Li Z, Tang X, Wang T, et al. Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice. Sci Rep. 2016;6:23912 pubmed publisher
  14. Pan H, Guan D, Liu X, Li J, Wang L, Wu J, et al. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res. 2016;26:190-205 pubmed publisher
  15. Wang Y, Kuramitsu Y, Tokuda K, Baron B, Kitagawa T, Akada J, et al. Gemcitabine induces poly (ADP-ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer. PLoS ONE. 2014;9:e109076 pubmed publisher